Shared Resource in Cellular Imaging

摘要 亨廷顿医学研究所（HMRI）细胞成像核心的愿景是 为调查人员提供尖端技术和专家技术支持， 显微镜观察和分析细胞解剖学和生理学，这是必不可少的 我们的多学科生物医学研究的各个方面。我们目前成像的一个主要差距 系统正在图像质量、吞吐量和可访问性之间取得平衡。由于 由于这些限制，HMRI的研究人员需要外包他们的明场， 免疫荧光需要昂贵且忙碌的核心设施。在这里，我们建议克服 这些工作流程和高成本限制通过提出一体化荧光显微镜， BZ-X800具有多种功能，将加强各实验室的细胞生物学项目， 核磁共振成像仪器将存放在指定的细胞成像核心设施中， 新建成的研究大楼的二楼，并将加快研究支持的5 NIH R 01赠款，用于强大的定量荧光和明场成像。芯会 由多个研究人员共享，并将加强本科生，STEM和 博士后教育项目这对于实现正在进行的 NIH资助的项目，以及计划未来的项目。这台一体化显微镜将有助于 我们的研究以前所未有的速度，并为理解和 治疗精神、神经和心脏疾病。

Abstract The vision of the Cellular Imaging Core of the Huntington Medical Research Institutes (HMRI) is to provide investigators access to cutting-edge technology, and expert technical support for microscopic observation and analysis of cellular anatomy and physiology that is essential for diverse aspects of our multidisciplinary biomedical research. A major gap in our current imaging systems is acquiring a balance between image quality, throughput, and accessibility. Due to these limitations, researchers at HMRI need to outsource their brightfield and immunofluorescence needs to costly and busy core facilities. Here we propose to overcome these workflow and high-cost limitations by proposing an all-in-one fluorescence microscope, BZ-X800 that has multiple features and will strengthen the cell biology projects in various labs at HMRI. The instrument will be housed in the Cellular Imaging Core facility designated to the second floor of the newly built research building and will accelerate research supported by 5 NIH R01 grants, on robust and quantitative fluorescence and brightfield imaging. The Core will be shared by multiple investigators and will strengthen the undergraduate, STEM and postdoctoral educational programs at HMRI. It will be critical for meeting the goals of ongoing NIH-funded projects, as well as planned future projects. This all-in-one microscope will facilitate our research at unprecedented speeds, and forge new avenues for understanding and treatment of psychiatric, neurological, and heart disease.