Shared Resource in Cellular Imaging

细胞成像共享资源

• 批准号: 10721473
• 负责人: Anju Vasudevan
• 金额: $ 28.33万
• 依托单位: HUNTINGTON MEDICAL RESEARCH INSTITUTES
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10721473
• 关键词: Acceleration; Biomedical Research; Cell physiology; Cellular biology; Core Facility; Education; Equilibrium; Floor; Fluorescence; Funding; Future; Goals; Grant; Heart Diseases; Huntington Disease; Image; Immunofluorescence Immunologic; Medical Research; Mental disorders; Microscope; Microscopic; Outsourcing; Postdoctoral Fellow; Psychiatric therapeutic procedure; Research; Research Institute; Research Personnel; Research Support; Resource Sharing; Speed; Subcellular Anatomy; Technology; United States National Institutes of Health; Vision; cellular imaging; cost; fluorescence microscope; forging; imaging system; instrument; meetings; multidisciplinary; nervous system disorder; programs; undergraduate student

Abstract The vision of the Cellular Imaging Core of the Huntington Medical Research Institutes (HMRI) is to provide investigators access to cutting-edge technology, and expert technical support for microscopic observation and analysis of cellular anatomy and physiology that is essential for diverse aspects of our multidisciplinary biomedical research. A major gap in our current imaging systems is acquiring a balance between image quality, throughput, and accessibility. Due to these limitations, researchers at HMRI need to outsource their brightfield and immunofluorescence needs to costly and busy core facilities. Here we propose to overcome these workflow and high-cost limitations by proposing an all-in-one fluorescence microscope, BZ-X800 that has multiple features and will strengthen the cell biology projects in various labs at HMRI. The instrument will be housed in the Cellular Imaging Core facility designated to the second floor of the newly built research building and will accelerate research supported by 5 NIH R01 grants, on robust and quantitative fluorescence and brightfield imaging. The Core will be shared by multiple investigators and will strengthen the undergraduate, STEM and postdoctoral educational programs at HMRI. It will be critical for meeting the goals of ongoing NIH-funded projects, as well as planned future projects. This all-in-one microscope will facilitate our research at unprecedented speeds, and forge new avenues for understanding and treatment of psychiatric, neurological, and heart disease.

抽象的 亨廷顿医学研究机构（HMRI）的细胞成像核心的视野是 为调查人员提供尖端技术的访问，以及专家技术支持 微观观察和分析细胞解剖学和生理学，这对于 我们多学科生物医学研究的各个方面。当前成像中的主要差距 系统正在获得图像质量，吞吐量和可访问性之间的平衡。由于 这些限制，HMRI的研究人员需要将其Brightfield外包和 免疫荧光需要昂贵而繁忙的核心设施。在这里我们建议克服 这些工作流程和高成本限制通过提出一个多合一的荧光显微镜， BZ-X800具有多个功能，可以加强各种实验室的细胞生物学项目 hmri。该仪器将安置在指定为蜂窝成像核心设施中 新建研究大楼的二楼，将加速研究，并得到5 NIH R01赠款，以鲁棒和定量的荧光和Brightfield成像。核心意愿 由多个调查人员共享，并将加强本科，STEM和 HMRI的博士后教育计划。实现正在进行的目标至关重要 NIH资助的项目以及计划的未来项目。这个多合一显微镜将有助于 我们以前所未有的速度进行研究，并为理解和 治疗精神病，神经和心脏病。