Chicken embryonic stem cells

鸡胚胎干细胞

• 批准号: 10719082
• 负责人: Qilong Ying
• 金额: $ 33.1万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-19 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10719082
• 关键词: Anser species; Back; Biological Assay; Birds; Breeding; Cell Line; Cell Lineage; Cells; Chick Embryo; Chicken Cells; Chickens; Chimera organism; Chimerism; Communities; Derivation procedure; Developmental Biology; Ducks; ES Cell Line; Embryo; Excision; Fibroblasts; Geese; Gene Expression Profiling; Gene Targeting; Generations; Genes; Genetic; Genetic Engineering; Germ Cells; Germ Layers; In Vitro; LIF gene; Meleagris gallopavo; Methods; Modeling; Mus; Production; Protein Kinase C Inhibitor; Quail; Rattus; Reporter; Research; Signal Pathway; Specific qualifier value; Structure of primordial sex cell; Struthio camelus; Study models; Technology; Testing; Time; Transforming Growth Factor beta; Transgenic Organisms; WNT Signaling Pathway; chicken egg; early embryonic stage; embryonic stem cell; germline stem cells; human disease; induced pluripotent stem cell; induced pluripotent stem cell technology; inhibitor; knockout gene; offspring; pluripotency; small molecule; small molecule inhibitor; stem cell differentiation; tool; transcription factor; transmission process; zebra finch

Abstract Chickens and their embryos provide a valuable research model for the study of developmental biology and human diseases. However, their application has been greatly limited due to the lack of tools to genetically modify chickens and other avian species. Recently, we have developed methods for the derivation of germline competent embryonic stem cells (ESCs) from chickens. The availability of germline competent chicken ESCs opens up a new avenue for producing genetically engineered chickens. In this project, we propose to establish an ESC-based platform for the efficient generation of chicken germline chimeras (Aim 1). We will also optimize methods for the generation of primordial germ cells (PGCs) from chicken ESCs. These chicken ESC-derived PGCs will be used to produce genetically modified chickens (Aim 2). In Aim 3, by taking the advantage of the chicken ESC culture condition we have established, we will develop methods for the generation of induced pluripotent stem cells (iPSCs) from chicken embryonic fibroblasts. Successful completion of these three aims will provide the avian research community with a powerful genetic toolkit for the production of transgenic and gene-targeted avian species and for the conservation of endangered avian species.

摘要