Chicken embryonic stem cells

摘要 鸡及其胚胎为发育生物学和胚胎的研究提供了有价值的研究模型 人类疾病。然而，由于缺乏研究基因的工具，它们的应用受到了极大的限制。 对鸡和其他禽类进行改良。最近，我们发展了一些方法来获得生殖系。 鸡的合格胚胎干细胞(ESCs)。种系合格鸡胚胎干细胞的可获得性 为生产转基因鸡开辟了一条新的途径。在这个项目中，我们建议建立 基于ESC的高效产生鸡种系嵌合体的平台(目标1)。我们还将优化 鸡胚胎干细胞原代生殖细胞的培养方法。这些鸡肉是由ESC衍生的 转基因鸡将被用于生产转基因鸡(目标2)。在目标3中，通过利用 我们已经建立了鸡胚胎干细胞的培养条件，我们将开发诱导产生的方法 从鸡胚胎成纤维细胞中分离出多能干细胞。圆满完成这三个目标 将为鸟类研究界提供一个强大的遗传工具包，用于生产转基因和 以基因为靶标的鸟类和保护濒危鸟类。

Abstract Chickens and their embryos provide a valuable research model for the study of developmental biology and human diseases. However, their application has been greatly limited due to the lack of tools to genetically modify chickens and other avian species. Recently, we have developed methods for the derivation of germline competent embryonic stem cells (ESCs) from chickens. The availability of germline competent chicken ESCs opens up a new avenue for producing genetically engineered chickens. In this project, we propose to establish an ESC-based platform for the efficient generation of chicken germline chimeras (Aim 1). We will also optimize methods for the generation of primordial germ cells (PGCs) from chicken ESCs. These chicken ESC-derived PGCs will be used to produce genetically modified chickens (Aim 2). In Aim 3, by taking the advantage of the chicken ESC culture condition we have established, we will develop methods for the generation of induced pluripotent stem cells (iPSCs) from chicken embryonic fibroblasts. Successful completion of these three aims will provide the avian research community with a powerful genetic toolkit for the production of transgenic and gene-targeted avian species and for the conservation of endangered avian species.