DERIVATION, PROPAGATION AND GENETIC MODIFICATION OF RAT EMBRYONIC STEM CELLS
大鼠胚胎干细胞的衍生、增殖和基因改造
基本信息
- 批准号:8435437
- 负责人:
- 金额:$ 38.25万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-01-01 至 2014-12-31
- 项目状态:已结题
- 来源:
- 关键词:AddressAffectAllelesAnimal ModelAnimalsBiologicalBreedingCellsChimera organismDerivation procedureDevelopmentDisease modelES Cell LineEmbryoFibroblastsGene TargetingGenesGeneticGoalsHaplotypesHumanKnock-outLeadMethodsModelingModificationMusPhysiologicalProductionRat StrainsRattusResearchResearch PersonnelStagingStudy modelsTechnologyTestingTransgenic Organismsbaseblastocystdesignembryonic stem cellgene functionhomologous recombinationhuman diseaseloss of functionmethod developmentpluripotencypublic health relevanceresearch studytransmission processvector
项目摘要
DESCRIPTION (provided by applicant): Rats are more relevant to humans than mice, both physiologically and pharmacologically. The central HYPOTHESIS behind the proposed research is that embryonic stem (ES) cell- based technologies developed for the mouse can be applied to create gene targeted rat models with rat ES cells. Robust and germline competent rat ES cells have been established (Cell 135:1299-310, 2008. Cell 153:1287-98). The overall GOAL of this project is to develop methods for the efficient and routine production of transgenic and gene knock- out rat models for the study of human disease. To achieve this goal, we will: (1) Develop methods to maximize germline transmission efficiency of rat ES cells. (2) Optimize conditions for robust propagation of germline competent rat ES cells. (3) Produce gene targeted rat ES cells and rats.
描述(申请人提供):无论在生理上还是药理上,大鼠都比小鼠更接近人类。这项拟议研究背后的中心假设是,为小鼠开发的基于胚胎干细胞的技术可以应用于创建具有大鼠胚胎干细胞的基因靶向大鼠模型。已经建立了健壮的、有生殖系能力的大鼠胚胎干细胞(Cell 135:1299-310,2008)。单元153:1287-98)。该项目的总体目标是开发有效和常规生产转基因和基因敲除大鼠模型的方法,用于研究人类疾病。为了实现这一目标,我们将:(1)开发最大化大鼠胚胎干细胞生殖系传递效率的方法。(2)优化繁殖条件,使生殖系感受态大鼠ES细胞健康繁殖。(3)制备基因靶向的大鼠胚胎干细胞和大鼠。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Qilong Ying其他文献
Qilong Ying的其他文献
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{{ truncateString('Qilong Ying', 18)}}的其他基金
Rapid egg bioreactor construction for Fabry disease therapeutics
用于法布里病治疗的快速鸡蛋生物反应器构建
- 批准号:
10481061 - 财政年份:2022
- 资助金额:
$ 38.25万 - 项目类别:
DERIVATION, PROPAGATION AND GENETIC MODIFICATION OF RAT EMBRYONIC STEM CELLS
大鼠胚胎干细胞的衍生、增殖和基因改造
- 批准号:
8214587 - 财政年份:2010
- 资助金额:
$ 38.25万 - 项目类别:
DERIVATION, PROPAGATION AND GENETIC MODIFICATION OF RAT EMBRYONIC STEM CELLS
大鼠胚胎干细胞的衍生、增殖和基因改造
- 批准号:
7791912 - 财政年份:2010
- 资助金额:
$ 38.25万 - 项目类别:
DERIVATION, PROPAGATION AND GENETIC MODIFICATION OF RAT EMBRYONIC STEM CELLS
大鼠胚胎干细胞的衍生、增殖和基因改造
- 批准号:
8005523 - 财政年份:2010
- 资助金额:
$ 38.25万 - 项目类别:
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