Multiplex Serodiagnostic Protein Microarray

多重血清诊断蛋白质微阵列

基本信息

  • 批准号:
    7657452
  • 负责人:
  • 金额:
    $ 74.76万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2008
  • 资助国家:
    美国
  • 起止时间:
    2008-08-01 至 2013-07-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): We have developed technology that enables rapid, comprehensive and high-throughput analysis of humoral immune responses to infectious disease antigens that is being used for subunit vaccine antigen discovery and to develop serodiagnostic tests. The method is based on printing microarray chips with proteins encoded by infectious microorganisms which cause medically important diseases in humans. Currently, we have expressed and printed more than 14,000 individual proteins from 6 bacteria, 17 viruses, and 2 parasites. The chips are probed with serum from infected patients and healthy controls to identify the dominant antibody responses, to determine the immunodominant antigen profile and identify a set of 10-20 serodiagnostic antigens for each infectious agent. By using a multiplicity of antigens for each infectious agent, serodiagnostic tests with statistically improved sensitivity and specificity can be configured. Here we will fabricate whole proteome microarrays for Coxiella burnetii, Rickettsia prowazekii, Brucella melitensis, Salmonella typhi, and non-homologous proteins from related strains of each agent. The arrays will be probed with serum from well-characterized infected subjects and healthy controls to identify the immunodominant and serodiagnostic antigen sets. We will also fabricate a chip containing 190 proteins from 16 arboviruses and 8 hantaviruses. The ability of this virus chip to distinguish well-characterized sera collected from humans and animals infected with the different viruses will be determined and the results compared with established assays done on the same samples. Deliverables generated from this project include plasmids, customized protein microarrays in a modular multiplex format, purified recombinant proteins and lateral flow (dipstick)/immunostrips devices, and will be accessible from the PSWRCE Protein Microarray Core on a recharge basis. These will be available to RCE investigators, government agencies and to private businesses through licensing agreements. The Protein Microarray Core will also provide chip probing and analysis services. Serodiagnostic antigen sets will be available for licensing to businesses interested in developing serodiagnostic tests. The immunodominant antigen sets, which may contain subunit vaccine antigens, will be available to commercial vaccine developers through licensing agreements. Multiplex serodiagnostic chips will be useful for determining whether military personnel or civilians, who enter a region where these agents are endemic, are exposed to any of them. They will also benefit public health monitoring activities to track annual changes in the prevalence of infections in endemic regions, and for monitoring the blood supply in developing countries. The chips will also be useful for assessing the spread of exposure in a population following a bioterrorism attack.
描述(由申请人提供):我们已经开发了一种技术,可以快速、全面和高通量地分析对传染病抗原的体液免疫反应,该技术被用于亚单位疫苗抗原发现和开发血清诊断测试。该方法是基于打印由传染性微生物编码的蛋白质的微阵列芯片,这些微生物会导致人类医学上重要的疾病。目前,我们已经表达和打印了来自6种细菌、17种病毒和2种寄生虫的14000多个单独的蛋白质。用感染患者和健康对照者的血清探测芯片,以确定显性抗体反应,确定免疫显性抗原谱,并为每种感染因子确定一组10-20种血清诊断抗原。通过对每种感染因子使用多种抗原,可以配置具有统计学上改进的敏感性和特异性的血清诊断测试。在这里,我们将制作完整的蛋白质组微阵列,用于伯纳氏杆菌、普氏立克次体、梅利特氏布鲁氏菌、伤寒沙门氏菌和来自每种病原体相关菌株的非同源蛋白。这些阵列将与具有良好特征的感染者和健康对照者的血清进行检测,以确定免疫优势和血清诊断抗原组。我们还将制作一个包含来自16种虫媒病毒和8种汉坦病毒的190种蛋白质的芯片。将确定该病毒芯片区分从感染不同病毒的人和动物收集的特征良好的血清的能力,并将结果与对相同样本进行的既定分析进行比较。该项目产生的可交付成果包括质粒、模块化多路格式的定制蛋白微阵列、纯化重组蛋白和侧流(试纸)/免疫条设备,并将在充电基础上从PSWRCE蛋白微阵列核心获取。这些将通过许可协议提供给RCE调查人员、政府机构和私营企业。蛋白质微阵列核心还将提供芯片探测和分析服务。对开发血清诊断测试感兴趣的企业将获得血清诊断抗原集的许可。免疫优势抗原组可能包含亚单位疫苗抗原,将通过许可协议提供给商业疫苗开发商。多路血清诊断芯片将有助于确定进入这些病原体流行地区的军事人员或平民是否暴露于其中任何一种病原体。它们还将有利于公共卫生监测活动,以跟踪流行地区感染流行率的年度变化,并监测发展中国家的血液供应。这种芯片还可用于评估生物恐怖袭击后人群的暴露程度。

项目成果

期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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PHILIP Louis FELGNER其他文献

PHILIP Louis FELGNER的其他文献

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{{ truncateString('PHILIP Louis FELGNER', 18)}}的其他基金

Predicting naturally acquired humoral immunity against malaria
预测针对疟疾的自然获得性体液免疫
  • 批准号:
    8465823
  • 财政年份:
    2012
  • 资助金额:
    $ 74.76万
  • 项目类别:
Predicting naturally acquired humoral immunity against malaria
预测针对疟疾的自然获得性体液免疫
  • 批准号:
    8373519
  • 财政年份:
    2012
  • 资助金额:
    $ 74.76万
  • 项目类别:
Predicting naturally acquired humoral immunity against malaria
预测针对疟疾的自然获得性体液免疫
  • 批准号:
    9312978
  • 财政年份:
    2012
  • 资助金额:
    $ 74.76万
  • 项目类别:
Predicting naturally acquired humoral immunity against malaria
预测针对疟疾的自然获得性体液免疫
  • 批准号:
    8720240
  • 财政年份:
    2012
  • 资助金额:
    $ 74.76万
  • 项目类别:
Protein Microarray
蛋白质微阵列
  • 批准号:
    8260269
  • 财政年份:
    2011
  • 资助金额:
    $ 74.76万
  • 项目类别:
Protein Microarray
蛋白质微阵列
  • 批准号:
    7675504
  • 财政年份:
    2009
  • 资助金额:
    $ 74.76万
  • 项目类别:
Multiplex Serodiagnostic Protein Microarray
多重血清诊断蛋白质微阵列
  • 批准号:
    7923917
  • 财政年份:
    2008
  • 资助金额:
    $ 74.76万
  • 项目类别:
Multiplex Serodiagnostic Protein Microarray
多重血清诊断蛋白质微阵列
  • 批准号:
    7458234
  • 财政年份:
    2008
  • 资助金额:
    $ 74.76万
  • 项目类别:
Multiplex Serodiagnostic Protein Microarray
多重血清诊断蛋白质微阵列
  • 批准号:
    8137655
  • 财政年份:
    2008
  • 资助金额:
    $ 74.76万
  • 项目类别:
Multiplex Serodiagnostic Protein Microarray
多重血清诊断蛋白质微阵列
  • 批准号:
    8307933
  • 财政年份:
    2008
  • 资助金额:
    $ 74.76万
  • 项目类别:

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