Flow Cytometry Core

这一至关重要的核心提供了对脾细胞、颈部淋巴结的常规和一致的分析 细胞、常驻细胞以及来源于中枢神经系统的炎性细胞。正如个别项目中所指出的那样， 精确分析感染期间侵入中枢神经系统的细胞以及驻留的中枢神经系统细胞 (少突胶质细胞、星形胶质细胞和小胶质细胞)是整个计划的关键组成部分。这包括 免疫过程中表达的表面标志、免疫调节分子和细胞因子的测定 驻留细胞和浸润性细胞类型的激活。细胞类型的精确识别和分离是 采集和分析以及通过分选纯化细胞所需的。此核心将提供高质量 控制每个项目所需的各种细胞分选策略的有效性和纯度。这一核心将 此外，通过确认kjone骨髓嵌合体的重组与动物核心(核心B)相互作用 以及繁殖群体中的遗传表型。因此，主要功能是：1)提供例程 获取和分析不同组织中的细胞组成及其表型变化；2)纯化细胞 从不同的小鼠组织中提取，用于基因图谱和体外免疫学检测；3)验证 骨髓重建；4)分析PBMC的基因分型；5)提供细胞质量控制 分离和纯净。所有这些任务的执行都履行了所有三个项目的基本职能。核心B 对于实现整个项目的总体科学目标至关重要。

This critically important core provides routine and consistent analysis of spleen cells, cervical lymph node cells, and resident as well as inflammatory cells derived from the CNS. As noted in the individual projects, precise analysis of the cells infiltrating the CNS during infection, as well as the resident CNS cells (oligodendroglia, astrocytes and microglia) are key components of the overall program. This includes measurement of surface markers, immune modulatory molecules, and cytokines expressed during immune activation by both resident and infiltrating cell types. Precise identification and separation of cell types is required for both acquisition and analysis and cell purification by sorting. This Core will provide quality control for efficacy and purity of various cell sorting strategies required for each project. This Core will furthermore interact with the Animal Core (Core B) by confirming reconstitution of kjone marrow chimeras and genetic phenotypes in the breeding colonies. The major functions are thus to: 1) provide routine acquisition and analysis of cell composition and their phenotypic changes in various tissues; 2) purify cells from various mouse tissues for gene profiling and immunological assays in vitro; 3) verify the efficacy of bone marrow reconstitution; 4) analyze PBMC for genotyping; and 5) provide quality control for cell separation and purity. Performance of all these tasks fulfills essential functions for all three projects. Core B is critical to accomplishing the overall scientific goals of the entire project.