Night Shift Work and Hormone Levels in Men

描述(申请人提供)：这项拟议的研究的主要目标是确定夜间工作是否与健康男性样本中的下列各项有关：尿6-硫代谢素浓度降低；血清睾酮、二氢睾酮(DHT)、3-雄二醇葡萄糖醛酸脂、脱氢表雄酮(DHEA)硫酸盐、雌酮和雌二醇浓度升高；下列雄激素代谢物尿液浓度升高：结合睾酮、DHT、黄体酮、雄酮、雄二醇和DHEA；以及血清性激素结合球蛋白浓度降低。这项研究还将调查：1)在夜间工作的男性中，白天睡眠期间的尿褪黑激素水平是否低于夜间睡眠，而上述雄激素和代谢物的血清和尿液水平是否高于夜间睡眠；2)人类生物钟基因的特定多态是否与个人适应夜班工作的能力有关。西雅图大都市区将招聘大约200名上夜班的男性医护人员和150名上白班的男性医护人员。符合条件的参与者必须是22-55岁，每周至少工作20小时，不服用激素制剂，没有前列腺癌病史。这项研究将允许夜班与白班工人的受试者之间的比较，以及夜班工人中白天睡眠与夜间睡眠的受试者内的比较。参与包括面谈、适应倒班工作能力的评估，以及在工作和睡眠期间收集血液和尿液。睡眠质量和数量将通过动作记录仪在与样本收集相对应的睡眠期间进行测量。将收集可能与研究中的荷尔蒙相关的因素的信息，如酒精消费、药物使用和样本采集期间的日照时间。将对血清和尿液样本进行上述激素和代谢物的检测，并对血液样本进行人类时钟、PER2和PER3基因的特定多态的基因分型。这项研究将是第一次评估夜班工作对褪黑素和前列腺癌病因学中最感兴趣的激素的影响。研究结果将提供重要的新信息，说明正在出现的将夜班工作与癌症风险联系起来的证据的潜在生物学基础。

DESCRIPTION (provided by applicant): The primary objective of the proposed study is to determine whether working at night is associated with the following in a sample of healthy men: decreased urinary concentration of 6-sulphatoxymelatonin; increased serum concentrations of testosterone, dihydrotesterone (DHT), 3-a androstanediol glucuronide, dehydroepiandrosterone (DHEA) sulfate, estrone, and estradiol; increased urinary concentrations of the following androgen metabolites: conjugated testosterone, DHT, etiocholanolone, androsterone, androstanediol, and DHEA; and decreased serum concentration of sex hormone-binding globulin. The study will also investigate: 1) whether urinary levels of melatonin are lower and serum and urinary levels of the androgens and metabolites listed above are higher during daytime sleep relative to nighttime sleep among men who work at night; and 2) if specific polymorphisms of the human circadian clock genes are associated with an individual's ability to adapt to night shift work. Approximately 200 male health care workers who work the night shift and 150 who work the day shift will be recruited in the Seattle metropolitan area. Eligible participants must be 22-55 years old, employed for at least 20 hours/week, not taking hormone preparations, and have no history of prostate cancer. This study will allow for both between-subject comparisons of night shift v. day shift workers, and within-subject comparisons during day sleep v. night sleep among night shift workers. Participation includes an in-person interview, assessment of ability to adapt to shiftwork, and blood and urine collections during both work and sleep periods. Sleep quality and quantity will be measured via actigraphy during the sleep periods corresponding to sample collection. Information will be collected on factors that may be related to the hormones under study, such as alcohol consumption, medication use, and hours of daylight during sample collection. Serum and urine samples will be assayed for the hormones and metabolites listed above, and blood samples will be genotyped for specific polymorphisms of the human CLOCK, PER2, and PER3 genes. This study will be the first effort to assess the effects of night shift work on melatonin and the hormones of most interest in the etiology of prostate cancer. Results will provide important new information regarding the underlying biological basis of the emerging evidence linking night shift work with cancer risk.