Night Shift Work and Hormone Levels in Men

夜班工作与男性荷尔蒙水平

• 批准号: 7924720
• 负责人: SCOTT DAVIS
• 金额: $ 84.13万
• 依托单位: FRED HUTCHINSON CANCER RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-07-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7924720
• 关键词: Adherence; Affect; Age; Alcohol consumption; Androgens; Androsterone; Area; Arousal; Biological; Biological Assay; Blood; Blood specimen; Characteristics; Circadian Rhythms; Collection; Complement; Cortisone; Creatinine; Dehydroepiandrosterone Sulfate; Development; Disease; Employment; Epidemiologic Studies; Estradiol; Estrone; Etiocholanolone; Etiology; Europe; Exposure to; Genes; Genetic Polymorphism; Genotype; Glucuronides; Growth and Development function; Health; Health Personnel; Health Professional; High temperature of physical object; Hormones; Hour; Human; Hydrocortisone; Hypothalamic structure; Individual; Interview; Link; Malignant Neoplasms; Malignant neoplasm of prostate; Measurement; Measures; Medical History; Melatonin; North America; Occupations; Oral; Participant; Pattern; Persons; Pharmaceutical Preparations; Physical activity; Pituitary Gland; Police officer; Preparation; Principal Investigator; Prostate; Protocols documentation; Publishing; Questionnaires; Recording of previous events; Recruitment Activity; Regulation; Relative (related person); Reporting; Research Personnel; Risk; Role; SHBG gene; Sampling; Schedule; Self-Administered; Serum; Sex Hormone-Binding Globulin; Sleep; Specific qualifier value; Steroids; Stress; Structure; Temperature; Testing; Testosterone; Time; Tissues; Urine; Work; actigraphy; base; cancer risk; circadian pacemaker; cold temperature; dehydroepiandrosterone; design; diaries; eligible participant; experience; hormone regulation; indexing; instrument; interest; male health; malignant breast neoplasm; men; metropolitan; programs; reproductive hormone; sample collection; shift work; sleep onset; steroid hormone; urinary; working men

DESCRIPTION (provided by applicant): The primary objective of the proposed study is to determine whether working at night is associated with the following in a sample of healthy men: decreased urinary concentration of 6-sulphatoxymelatonin; increased serum concentrations of testosterone, dihydrotesterone (DHT), 3-a androstanediol glucuronide, dehydroepiandrosterone (DHEA) sulfate, estrone, and estradiol; increased urinary concentrations of the following androgen metabolites: conjugated testosterone, DHT, etiocholanolone, androsterone, androstanediol, and DHEA; and decreased serum concentration of sex hormone-binding globulin. The study will also investigate: 1) whether urinary levels of melatonin are lower and serum and urinary levels of the androgens and metabolites listed above are higher during daytime sleep relative to nighttime sleep among men who work at night; and 2) if specific polymorphisms of the human circadian clock genes are associated with an individual's ability to adapt to night shift work. Approximately 200 male health care workers who work the night shift and 150 who work the day shift will be recruited in the Seattle metropolitan area. Eligible participants must be 22-55 years old, employed for at least 20 hours/week, not taking hormone preparations, and have no history of prostate cancer. This study will allow for both between-subject comparisons of night shift v. day shift workers, and within-subject comparisons during day sleep v. night sleep among night shift workers. Participation includes an in-person interview, assessment of ability to adapt to shiftwork, and blood and urine collections during both work and sleep periods. Sleep quality and quantity will be measured via actigraphy during the sleep periods corresponding to sample collection. Information will be collected on factors that may be related to the hormones under study, such as alcohol consumption, medication use, and hours of daylight during sample collection. Serum and urine samples will be assayed for the hormones and metabolites listed above, and blood samples will be genotyped for specific polymorphisms of the human CLOCK, PER2, and PER3 genes. This study will be the first effort to assess the effects of night shift work on melatonin and the hormones of most interest in the etiology of prostate cancer. Results will provide important new information regarding the underlying biological basis of the emerging evidence linking night shift work with cancer risk.

描述（由申请人提供）：拟议的研究的主要目的是确定健康男性样本中是否与以下样本相关：尿液浓度降低6-磺破合蛋白酶；睾丸激素，二氢酮（DHT），3-A雄烷基葡萄糖醛酸，脱氢表雄酮（DHEA）硫酸盐，雌酮和雌二醇的血清浓度增加；以下雄激素代谢产物的尿液浓度增加：共轭睾丸激素，DHT，etiocholanolone，雄激素，雄激素，雄激素二醇和DHEA；并降低了性激素结合球蛋白的血清浓度。该研究还将调查：1）褪黑激素的尿液水平是否较低，雄激素和代谢物的血清和尿位水平在白天睡眠中相对于晚上工作的男性的夜间睡眠而较高； 2）如果人类昼夜节律基因的特定多态性与个人适应夜班工作的能力有关。大约200名在夜班工作的男性卫生保健工作者，在西雅图大都会地区招募了150名工作日常工作。符合条件的参与者必须年满22-55岁，每周至少使用20个小时，不服用激素准备，并且没有前列腺癌的病史。这项研究将允许对夜班诉Day Shift工人的受试者之间的比较，以及在夜班工人中的夜间睡眠中的夜间睡眠中的受试者内部比较。参与包括面对面的访谈，适应转移工作的能力的评估以及在工作和睡眠期间的血液和尿液收集。睡眠质量和数量将在与样品收集相对应的睡眠期间通过精神分裂进行测量。将收集有关可能与正在研究的激素有关的因素，例如饮酒，药物使用以及样本收集期间的白天时间。血清和尿液样品将用于上面列出的激素和代谢产物，并将血液样本用于人类时钟，PER2和PER3基因的特定多态性的基因分型。这项研究将是评估夜班工作对褪黑激素的影响以及在前列腺癌病因中最感兴趣的激素的作用。结果将为有关夜班工作与癌症风险联系起来的新兴证据的基本生物学基础提供重要的新信息。