In vivo tracking of bioluminescent markers of circadian rhythms in behaving animals
In vivo tracking of bioluminescent markers of circadian rhythms in behaving animals
批准号:
10730688
负责人:
MARY E HARRINGTON
金额:
$39.63万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2017
资助国家:
美国
项目状态:
未结题
起止时间:
2017-09-15 至 2026-09-17
关键词:
AblationAnimal ModelAnimalsBiological AssayBioluminescenceBrainCardiovascular systemCircadian RhythmsCircadian desynchronyDataGene ExpressionGoalsHealthHistologyImpairmentInterventionLaboratory miceLengthLesionLightLiverMeasuresMentorsMetabolicMethodsMotor ActivityMusOpsinPeriodicityPeripheralPhysiologyReporterReportingResearchRoleScienceSkinSourceStructureStudy modelsSystemTestingTissuesWomancareercircadiancircadian pacemakerimprovedin vivoin vivo imaging systemlight effectssuprachiasmatic nucleustoolundergraduate student
中文摘要
项目总结
英文摘要
Project Summary
Circadian rhythms are fundamental in sculpting physiology, and their alignment is essential for
optimal health. We have gained deep understanding of the cellular basis as well as the function of the
central circadian clock of the suprachiasmatic nuclei (SCN), but we don’t yet understand how the
SCN interacts with varied peripheral oscillators, and how these peripheral clocks integrate information
from SCN- and non-SCN-inputs to maintain internal alignment as well as entrainment to 24h cycles.
Here we will study two peripheral oscillators, the liver and skin. We will develop an animal model for
study of the role of these peripheral clocks within the circadian system, to determine their entrainment
capabilities.
The central hypothesis of this proposal is that peripheral clocks retain some rhythmic
capabilities when the SCN central clock is impaired, possibly including photic entrainment. The long-
term goal of this research is to understand the inter-dynamics of circadian clocks throughout the
mammalian body and, ultimately, discover new tools to lessen the impact of circadian misalignment
on health.
Our research will first aim to characterize our new skin circadian clock reporter line K14 Cre;
DBPKI/+ mice. We will more fully characterize our new mouse line using IVIS imaging, assays, and
histology to rigorously determine sources of the bioluminescence recorded. Second, we will aim to
test photic entrainment of skin and liver circadian rhythms in vivo in mice with or without brain central
clock function. This aim will test the entrainment of skin and liver using methods for long-term
circadian bioluminescent reporting from living animals, first in intact mice and then in mice with
suprachiasmatic nuclei (SCN) ablation. Third, we will determine if these peripheral tissues show a
wider range of entrainment than the central clock. We will test the ability for circadian rhythms of gene
expression of K14 Cre; DBPKI/+ and Alb Cre; DBPKI/+ mice to entrain to cycles of varied cycle lengths (“T
cycles”), to assess the range of entrainment, as compared with that of locomotor activity, using both
intact and SCN-lesion mice.
Completion of these studies will dramatically alter our understanding of the mammalian
circadian system to include potentially photoreceptive peripheral clocks. The research will be
conducted by diverse and engaged undergraduate women, with structured mentoring to encourage
continued careers in biomedical science.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
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批准号:10512955
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资助金额:$8.98万
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财政年份:2022
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负责人:MARY E HARRINGTON
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Developing Training Materials for Experimental Rigor in Neuroscience
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批准号:10665056
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Building foundations for a neurobiology of fatigue: validating an animal model
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批准号:8427275
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资助金额:$15.42万
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财政年份:2012
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批准号:8242265
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财政年份:2012
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依托单位:
Circadian clock suppression in cancer-related fatigue
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批准号:7386126
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项目类别:
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资助金额:$17.1万
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财政年份:2008
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负责人:MARY E HARRINGTON
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依托单位:
Circadian clock suppression in cancer-related fatigue
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批准号:7595251
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项目类别:
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资助金额:$14.05万
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财政年份:2008
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负责人:MARY E HARRINGTON
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依托单位:
Potentiation of photic circadian clock phase shifts
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批准号:7454623
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项目类别:
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资助金额:$0.93万
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财政年份:2006
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负责人:MARY E HARRINGTON
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依托单位:
Potentiation of photic circadian clock phase shifts
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批准号:7075789
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项目类别:
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资助金额:$19.52万
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财政年份:2006
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负责人:MARY E HARRINGTON
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依托单位:
NEURAL BASIS OF BIOLOGICAL RHYTHMS
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批准号:2259646
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项目类别:
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资助金额:$7.02万
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财政年份:1994
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负责人:MARY E HARRINGTON
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依托单位:
NEURAL BASIS OF BIOLOGICAL RHYTHMS
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批准号:2460448
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项目类别:
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资助金额:$7.02万
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财政年份:1994
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负责人:MARY E HARRINGTON
-
依托单位:
NEURAL BASIS OF BIOLOGICAL RHYTHMS
-
批准号:2259645
-
项目类别:
-
资助金额:$7.02万
-
财政年份:1994
-
负责人:MARY E HARRINGTON
-
依托单位:
NEURAL BASIS OF BIOLOGICAL RHYTHMS
-
批准号:2750765
-
项目类别:
-
资助金额:$7.02万
-
财政年份:1994
-
负责人:MARY E HARRINGTON
-
依托单位:
NEURAL BASIS OF BIOLOGICAL RHYTHMS
-
批准号:2259644
-
项目类别:
-
资助金额:$7.02万
-
财政年份:1994
-
负责人:MARY E HARRINGTON
-
依托单位:
NEURAL BASIS OF BIOLOGICAL RHYTHMS
-
批准号:2502957
-
项目类别:
-
资助金额:$18.38万
-
财政年份:1988
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负责人:MARY E HARRINGTON
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依托单位:
NEURAL BASIS OF BIOLOGICAL RHYTHMS
-
批准号:2265960
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项目类别:
-
资助金额:$15.18万
-
财政年份:1988
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负责人:MARY E HARRINGTON
-
依托单位:
NEURAL BASIS OF BIOLOGICAL RHYTHMS
-
批准号:3477534
-
项目类别:
-
资助金额:$8.42万
-
财政年份:1988
-
负责人:MARY E HARRINGTON
-
依托单位:
NEURAL BASIS OF BIOLOGICAL RHYTHMS
-
批准号:3477535
-
项目类别:
-
资助金额:$7.52万
-
财政年份:1988
-
负责人:MARY E HARRINGTON
-
依托单位:
NEURAL BASIS OF BIOLOGICAL RHYTHMS
-
批准号:6149353
-
项目类别:
-
资助金额:$2.5万
-
财政年份:1988
-
负责人:MARY E HARRINGTON
-
依托单位:
NEURAL BASIS OF BIOLOGICAL RHYTHMS
-
批准号:2265962
-
项目类别:
-
资助金额:$16.07万
-
财政年份:1988
-
负责人:MARY E HARRINGTON
-
依托单位:
NEURAL BASIS OF BIOLOGICAL RHYTHMS
-
批准号:3477533
-
项目类别:
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资助金额:$9.14万
-
财政年份:1988
-
负责人:MARY E HARRINGTON
-
依托单位:
海外基金