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Tools for mapping the tubulin landscape

绘制微管蛋白景观的工具

基本信息

批准号：
10785950
负责人：
Jeffrey Kyle Moore
金额：
$ 15.52万
依托单位：
UNIVERSITY OF COLORADO DENVER
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-09-15 至 2025-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10785950
关键词：
Amino Acid Sequence Antibodies Architecture Area Axon Binding Biochemical Brain Brain Diseases C-terminal Cells Chemicals Chimeric Proteins Code Cytoplasm Cytoskeleton Data Dendrites Development Disease Distal Elements Environment Exhibits Future Gene Family Goals Heterogeneity Human Genome Imaging Device Intrabody Kinesin Lead Malignant Neoplasms Maps Messenger RNA Microtubule-Associated Proteins Microtubules Modality Molecular Monoclonal Antibodies Morphogenesis Motor Movement Muscle Neurodevelopmental Disorder Neurons Nuclear Organelles Paclitaxel Pattern Pharmaceutical Preparations Play Population Positioning Attribute Post-Translational Protein Processing Process Recombinant Antibody Recombinants Refractory Regulation Research Research Personnel Role Signal Transduction Site Structural defect Surface Tail Testing Therapeutic Time Translations Tubulin Variant Visualization alpha Tubulin beta Tubulin brain malformation cancer cell cell motility cell type cost developmental disease dimer extracellular gain of function insight nerve supply programs recruit response success superresolution microscopy tool ubiquitin-protein ligase

项目摘要

Tools for mapping the tubulin landscape Project Summary: do Over information This tubulins. tubulin neuronal recombinant research whether new are essential to the architectural complexity and signaling efficiency of neurons. However, we not fully understand how neurons build specialized microtubule networks at the right place and right time. the past 15 years, we have come to appreciate that the tubulin subunits within microtubules carry in the form of posttranslational modifications that regulate the assembly and function of networks. proposal extends this concept to the level of the tubulin isotypes – the gene families that encode α - and β Our goal is to develop new tools that will enable the first-ever visualization and manipulation of a isotype in living cells. proposal will create tools that will advance our understanding of the β-tubulin isotype, TUBB3, during morphogenesis, but will also have broad impacts across many labs. Our approach will 1) create a form of the TUBB3-binding antibody Tuj1 that can be shared with other labs and bring down costs, 2) create the f irst-ever intrabody for studying a tubulin isotype in living ells, 3) determine TUBB3 exhibits biased enrichment to regions of the neuron or to microtubules with PTMs, 4) create a tool for rapid and selective depletion of TUBB3 in cells Microtubules - This c . The success of this proposal will set the stage for our future R01 proposal, which will elucidate the mechanisms that regulate TUBB3 localization and function during development, and how these are disrupted in disease.

绘制微管蛋白景观的工具