Tools for mapping the tubulin landscape

绘制微管蛋白地形图的工具 项目总结： 做 完毕 信息 这 微管蛋白。 微管蛋白 神经元 重组 研究 是否 新的 对于神经元的结构复杂性和信号效率是必不可少的。然而，我们 不完全了解神经元如何在正确的地点和正确的时间建立专门的微管网络。 在过去的15年里，我们逐渐认识到微管中的微管蛋白亚基携带 以翻译后修饰的形式，调节网络的组装和功能。 Proposal将这一概念扩展到微管蛋白同种类型-编码α的基因家族-和β的水平 我们的目标是开发新的工具，使有史以来第一次可视化和操作 活细胞中的同型。 提案将创建工具，以促进我们对β-微管蛋白亚型TUBB3的理解 形态发生，但也将在许多实验室产生广泛影响。我们的方法将1)创建一个 TUBB3结合抗体Tuj1的形式，可与其他实验室共享并关闭 成本，2)创造第一个用于研究活细胞中微管蛋白亚型的体内，3)确定 TUBB3通过PTMS表现出对神经元区域或微管的偏向浓缩，4)创建 快速选择性耗尽细胞内TUBB3的工具 微管 - 这 C 。这项提议的成功将为 我们未来的R01提案，它将阐明调节TUBB3本地化和功能的机制 在发育过程中，以及这些在疾病中是如何被破坏的。

Tools for mapping the tubulin landscape Project Summary: do Over information This tubulins. tubulin neuronal recombinant research whether new are essential to the architectural complexity and signaling efficiency of neurons. However, we not fully understand how neurons build specialized microtubule networks at the right place and right time. the past 15 years, we have come to appreciate that the tubulin subunits within microtubules carry in the form of posttranslational modifications that regulate the assembly and function of networks. proposal extends this concept to the level of the tubulin isotypes – the gene families that encode α - and β Our goal is to develop new tools that will enable the first-ever visualization and manipulation of a isotype in living cells. proposal will create tools that will advance our understanding of the β-tubulin isotype, TUBB3, during morphogenesis, but will also have broad impacts across many labs. Our approach will 1) create a form of the TUBB3-binding antibody Tuj1 that can be shared with other labs and bring down costs, 2) create the f irst-ever intrabody for studying a tubulin isotype in living ells, 3) determine TUBB3 exhibits biased enrichment to regions of the neuron or to microtubules with PTMs, 4) create a tool for rapid and selective depletion of TUBB3 in cells Microtubules - This c . The success of this proposal will set the stage for our future R01 proposal, which will elucidate the mechanisms that regulate TUBB3 localization and function during development, and how these are disrupted in disease.