Biocontainment Research Support Service(s) Core

生物防护研究支持服务核心

• 批准号: 10793830
• 负责人: Rachel M Olson
• 金额: $ 94.49万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-18 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10793830
• 关键词: 2019-nCoV; Advanced Development; Aerosols; Animal Model; Animals; Area; Biological Assay; Blood Chemical Analysis; COVID-19 pandemic; Cavia; Cells; Chemicals; Collaborations; Communicable Diseases; Communication; Communities; Containment; Controlled Environment; Core Facility; Custom; Data; Dedications; Development; Diagnostic; Emerging Communicable Diseases; Ensure; Equipment; Evaluation; Event; Family suidae; Ferrets; Flow Cytometry; Funding; Goals; Goat; Growth; Hamsters; Human Resources; Image; Immune response; Immunity; Immunology; Infection; Infectious Diseases Research; Inhalation Exposure; Investments; Laboratories; Maintenance; Manufacturer; Methodology; Methods; Microbiology; Microscopy; Missouri; Modeling; Monitor; Mus; Mutant Strains Mice; National Institute of Allergy and Infectious Disease; Oryctolagus cuniculus; Persons; Procedures; Productivity; Public Health; Rattus; Recovery; Reproducibility; Research; Research Personnel; Research Support; Resource Development; Resources; Route; Safety; Sampling; Scientist; Secure; Services; Sheep; Specialist; Speed; Structure; System; Technical Expertise; Technology; Time; Training; Transgenic Animals; Translating; United States National Institutes of Health; Universities; Viral; Work; Workforce Development; biodefense; biosafety level 3 facility; cytokine; emerging pathogen; experience; experimental study; human disease; human model; in vitro Assay; instrumentation; medical countermeasure; new outbreak; next generation; novel; novel vaccines; pathogen; pathogen exposure; pathogenic bacteria; pathogenic virus; pre-clinical; preclinical development; priority pathogen; programs; real-time images; recruit; research and development; response; vector transmission

Project Summary Core 3: Biocontainment Research Support Services Core The objective of the Biocontainment Research Support Services Core (BRSSC, Core 3) is to provide sustainable research resources that facilitate the pre-clinical development of medical countermeasures against emerging and re-emerging pathogens. To achieve this, the BRSSC provides world-class resources in the priority areas of aerobiology, animal modeling, immunology, imaging, and microbiology for BSL-3 bacterial and viral pathogens. The BRSSC serves the needs of MU as well as NIAID and the RBL-NBL network by focusing its capabilities to provide technical expertise under BSL-3 containment for NIAID priority pathogens and the evaluation of novel vaccines, treatments, and diagnostics. The BRSSC closely collaborates with other relevant Research Services at MU, including but not limited to the NIH-funded Resource and Research Centers for Mutant Mouse, Rat, and Swine to develop and refine novel transgenic animal models for priority pathogens such as SARS-CoV-2. The technical staff of the BRSSC is a dedicated team for conducting and supporting experiments using advanced technology, high-precision instrumentation on live samples. Added layers of primary and secondary containment allow for safe handling and usage of the unique support services that are offered in the BRSSC. For example, direct access from the vivarium to the class III glovebox and its inhalation exposure chamber eliminates the need to move infected animals through corridors or other space that may expose people. In addition, there is a high- speed cell sorter, housed within a manufacturer-supplied class II BSC, that allows recovery of live cells infected with a BSL-3 pathogen for downstream analysis. The Lionheart fluorescent microscopy system allows real-time imaging of environmentally-controlled live BSL-3 samples in multi-well format, and is useful for optimization of viral growth conditions and other live and in vitro assays without need for pathogen inactivation. Further imaging and other instrumentation available in the ABSL-3 allows for longitudinal monitoring of the infection, blood chemistry and immune responses without need to chemically inactivate sample, thereby reducing time and unwanted impact for the procedure. All of these examples illustrate the capabilities for safe conduct of BSL-3 research using advanced technology instrumentation that is available through the BRSSC. The collaborative effort between the dedicated teams of Cores 1, 2 and 3 of this UC7 application combined with regular interactions with the NIAID RBL-NBL network will maximize best practices and usage of these unique resources. To create sustainability, the BRSSC is committed to training diverse technical professionals and scientists in BSL-3/ACL- 3/ABSL-3 research. Through management of the critical resources at the LIDR, the BRSSC helps advance the development of novel medical countermeasures against BSL-3 pathogens.

项目概要 核心 3：生物防护研究支持服务核心 生物防护研究支持服务核心（BRSSC，核心 3）的目标是提供可持续的 促进针对新出现的医学对策的临床前开发的研究资源 和重新出现的病原体。为了实现这一目标，BRSSC 在以下优先领域提供世界一流的资源： BSL-3 细菌和病毒病原体的空气生物学、动物模型、免疫学、成像和微生物学。 BRSSC 通过集中其能力来满足 MU 以及 NIAID 和 RBL-NBL 网络的需求 在 BSL-3 遏制下为 NIAID 优先病原体提供技术专业知识并评估新型 疫苗、治疗和诊断。 BRSSC 与其他相关研究机构密切合作 密西根大学，包括但不限于 NIH 资助的突变小鼠、大鼠和突变资源和研究中心 猪开发和完善针对 SARS-CoV-2 等优先病原体的新型转基因动物模型。这 BRSSC 的技术人员是一个专门的团队，使用先进的技术进行和支持实验 技术，实时样品的高精度仪器。添加了一级和二级安全壳层 允许安全处理和使用 BRSSC 中提供的独特支持服务。例如， 从饲养室直接进入 III 级手套箱及其吸入暴露室，无需 将受感染的动物移过走廊或其他可能使人暴露的空间。此外，还有一个高 快速细胞分选仪，安装在制造商提供的 II 类 BSC 内，可回收感染的活细胞 与 BSL-3 病原体进行下游分析。 Lionheart 荧光显微镜系统可实现实时 以多孔形式对环境控制的活体 BSL-3 样品进行成像，可用于优化 病毒生长条件和其他活体和体外测定，无需病原体灭活。进一步成像 和 ABSL-3 中可用的其他仪器可以纵向监测感染、血液 化学和免疫反应，无需对样品进行化学灭活，从而减少时间和 对程序产生不必要的影响。所有这些例子都说明了 BSL-3 安全运行的能力 使用 BRSSC 提供的先进技术仪器进行研究。协作式 该 UC7 应用程序核心 1、2 和 3 的专业团队之间的努力结合定期互动 与 NIAID RBL-NBL 网络的合作将最大限度地提高这些独特资源的最佳实践和利用。创造 可持续发展，BRSSC 致力于培训 BSL-3/ACL- 领域的多元化技术专业人员和科学家 3/ABSL-3 研究。通过管理 LIDR 的关键资源，BRSSC 帮助推进 开发针对 BSL-3 病原体的新型医学对策。