Biocontainment Research Support Service(s) Core

生物防护研究支持服务核心

• 批准号: 10793830
• 负责人: Rachel M Olson
• 金额: $ 94.49万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-18 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10793830
• 关键词: 2019-nCoV; Advanced Development; Aerosols; Animal Model; Animals; Area; Biological Assay; Blood Chemical Analysis; COVID-19 pandemic; Cavia; Cells; Chemicals; Collaborations; Communicable Diseases; Communication; Communities; Containment; Controlled Environment; Core Facility; Custom; Data; Dedications; Development; Diagnostic; Emerging Communicable Diseases; Ensure; Equipment; Evaluation; Event; Family suidae; Ferrets; Flow Cytometry; Funding; Goals; Goat; Growth; Hamsters; Human Resources; Image; Immune response; Immunity; Immunology; Infection; Infectious Diseases Research; Inhalation Exposure; Investments; Laboratories; Maintenance; Manufacturer; Methodology; Methods; Microbiology; Microscopy; Missouri; Modeling; Monitor; Mus; Mutant Strains Mice; National Institute of Allergy and Infectious Disease; Oryctolagus cuniculus; Persons; Procedures; Productivity; Public Health; Rattus; Recovery; Reproducibility; Research; Research Personnel; Research Support; Resource Development; Resources; Route; Safety; Sampling; Scientist; Secure; Services; Sheep; Specialist; Speed; Structure; System; Technical Expertise; Technology; Time; Training; Transgenic Animals; Translating; United States National Institutes of Health; Universities; Viral; Work; Workforce Development; biodefense; biosafety level 3 facility; cytokine; emerging pathogen; experience; experimental study; human disease; human model; in vitro Assay; instrumentation; medical countermeasure; new outbreak; next generation; novel; novel vaccines; pathogen; pathogen exposure; pathogenic bacteria; pathogenic virus; pre-clinical; preclinical development; priority pathogen; programs; real-time images; recruit; research and development; response; vector transmission

Project Summary Core 3: Biocontainment Research Support Services Core The objective of the Biocontainment Research Support Services Core (BRSSC, Core 3) is to provide sustainable research resources that facilitate the pre-clinical development of medical countermeasures against emerging and re-emerging pathogens. To achieve this, the BRSSC provides world-class resources in the priority areas of aerobiology, animal modeling, immunology, imaging, and microbiology for BSL-3 bacterial and viral pathogens. The BRSSC serves the needs of MU as well as NIAID and the RBL-NBL network by focusing its capabilities to provide technical expertise under BSL-3 containment for NIAID priority pathogens and the evaluation of novel vaccines, treatments, and diagnostics. The BRSSC closely collaborates with other relevant Research Services at MU, including but not limited to the NIH-funded Resource and Research Centers for Mutant Mouse, Rat, and Swine to develop and refine novel transgenic animal models for priority pathogens such as SARS-CoV-2. The technical staff of the BRSSC is a dedicated team for conducting and supporting experiments using advanced technology, high-precision instrumentation on live samples. Added layers of primary and secondary containment allow for safe handling and usage of the unique support services that are offered in the BRSSC. For example, direct access from the vivarium to the class III glovebox and its inhalation exposure chamber eliminates the need to move infected animals through corridors or other space that may expose people. In addition, there is a high- speed cell sorter, housed within a manufacturer-supplied class II BSC, that allows recovery of live cells infected with a BSL-3 pathogen for downstream analysis. The Lionheart fluorescent microscopy system allows real-time imaging of environmentally-controlled live BSL-3 samples in multi-well format, and is useful for optimization of viral growth conditions and other live and in vitro assays without need for pathogen inactivation. Further imaging and other instrumentation available in the ABSL-3 allows for longitudinal monitoring of the infection, blood chemistry and immune responses without need to chemically inactivate sample, thereby reducing time and unwanted impact for the procedure. All of these examples illustrate the capabilities for safe conduct of BSL-3 research using advanced technology instrumentation that is available through the BRSSC. The collaborative effort between the dedicated teams of Cores 1, 2 and 3 of this UC7 application combined with regular interactions with the NIAID RBL-NBL network will maximize best practices and usage of these unique resources. To create sustainability, the BRSSC is committed to training diverse technical professionals and scientists in BSL-3/ACL- 3/ABSL-3 research. Through management of the critical resources at the LIDR, the BRSSC helps advance the development of novel medical countermeasures against BSL-3 pathogens.

项目摘要 核心3：生物疗养研究支持服务核心 生物疗法研究支持服务核心（BRSSC，核心3）的目的是提供可持续性 研究资源，促进临时发展医学对策的研究资源 并重新出现病原体。为此，BRSSC在优先领域的优先领域提供了世界一流的资源 BSL-3细菌和病毒病原体的特技生物学，动物建模，免疫学，成像和微生物学。 BRSSC通过将其功能集中到MU以及NIAID和RBL-NBL网络的需求 提供NIAID优先病原体的BSL-3遏制下的技术专业知识和新颖的评估 疫苗，治疗和诊断。 BRSSC与其他相关研究服务密切合作 在MU，包括但不限于NIH资助的资源和突变小鼠，大鼠和 猪开发和完善新的转基因动物模型，以用于优先病原体，例如SARS-COV-2。这 BRSSC的技术人员是一个专门的团队，用于使用高级实验进行和支持实验 技术，现场样品上的高精度仪器。添加了主要和次要遏制层 允许安全处理和使用BRSSC中提供的独特支持服务。例如， 直接从Vivarium到III类手套箱及其吸入室消除了需求 将受感染的动物通过走廊或可能暴露人们的其他空间。此外，还有一个高 放置在制造商提供的II类BSC中的速度单元格拉刀，该分类器可恢复感染的活细胞 用BSL-3病原体进行下游分析。 Lionheart荧光显微镜系统允许实时 以多孔格式的环境控制的实时BSL-3样品进行成像，可用于优化 病毒生长条件以及其他生物和体外测定，无需病原体失活。进一步的成像 ABSL-3中可用的其他仪器允许对感染，血液进行纵向监测 化学和免疫反应无需化学灭活样品，从而减少时间和 对程序的不良影响。所有这些示例都说明了安全进行BSL-3的能力 使用BRSSC可用的先进技术仪器进行研究。协作 核心1、2和3的专用团队之间的努力与定期互动相结合 借助NIAID RBL-NBL网络将最大程度地提高这些独特资源的最佳实践和使用。创建 可持续性，BRSSC致力于培训BSL-3/ACL-的各种技术专业人士和科学家 3/absl-3研究。通过管理LIDR的关键资源，BRSSC有助于进步 开发针对BSL-3病原体的新型医学对策。