Characterization of non-canonical regulatory pathways in the Caulobacter NtrYX signaling system

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Project Summary/Abstract All bacteria must sense and respond to internal and external signals to thrive in diverse environments. This sensing is often achieved using protein-based two-component signaling systems. Our funded parent project is developing the Caulobacter crescentus NtrY-NtrX (NtrYX) two-component signaling system as a model for understanding complex bacterial signaling pathways. Our aims seek to understand this atypical two-component signaling system at multiple levels, from regulation of signal flow to changes in transcription. Although our aims employ diverse biochemical and genetic methods, at their core they all require bacterial culture. Thus, a functional and reliable autoclave for the preparation of growth media, sterile glassware, and sterile consumables is key for the success of our project goals. This proposal seeks an administrative supplement to replace our broken autoclave with a functional, high quality, and high-capacity autoclave appropriate for our laboratory space. This new autoclave will be capable of meeting all sterilization demands for our parent grant and will also help support additional microbial-related research and teaching in our department.