Characterization of non-canonical regulatory pathways in the Caulobacter NtrYX signaling system

柄杆菌 NtrYX 信号系统中非典型调控途径的表征

• 批准号: 10794646
• 负责人: Benjamin J Stein
• 金额: $ 5.09万
• 依托单位: UNIVERSITY OF TENNESSEE CHATTANOOGA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-20 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10794646
• 关键词: Administrative Supplement; Agriculture; Bacteria; Biochemical; Caulobacter; Caulobacter crescentus; Complex; Culture Media; Development; Educational process of instructing; Environment; Funding; Future; Genetic; Genetic Transcription; Goals; Health; Homeostasis; Human; Infection; Laboratories; Methods; Microbe; Modeling; Organism; Preparation; Proliferating; Proteins; Regulation; Regulatory Pathway; Research; Signal Pathway; Signal Transduction; Sterility; Sterilization; System; Work; antimicrobial; environmental change; experience; meetings; microbial; parent grant; parent project; success

Project Summary/Abstract All bacteria must sense and respond to internal and external signals to thrive in diverse environments. This sensing is often achieved using protein-based two-component signaling systems. Our funded parent project is developing the Caulobacter crescentus NtrY-NtrX (NtrYX) two-component signaling system as a model for understanding complex bacterial signaling pathways. Our aims seek to understand this atypical two-component signaling system at multiple levels, from regulation of signal flow to changes in transcription. Although our aims employ diverse biochemical and genetic methods, at their core they all require bacterial culture. Thus, a functional and reliable autoclave for the preparation of growth media, sterile glassware, and sterile consumables is key for the success of our project goals. This proposal seeks an administrative supplement to replace our broken autoclave with a functional, high quality, and high-capacity autoclave appropriate for our laboratory space. This new autoclave will be capable of meeting all sterilization demands for our parent grant and will also help support additional microbial-related research and teaching in our department.

项目摘要/摘要 所有细菌都必须感知并响应内部和外部信号，以在不同的环境中蓬勃发展。 这种传感通常是使用基于蛋白质的两组分组信号系统实现的。我们资助的家长项目 正在开发Caulobacter Crescentus ntry-Ntrx（Ntryx）两个组件信号系统作为模型 了解复杂的细菌信号通路。我们的目标试图理解这种非典型的两个组成部分 信号系统在多个级别上，从信号流的调节到转录的变化。虽然我们的目标是 采用多种生化和遗传学方法，它们的核心都需要细菌培养。因此， 用于制备增长媒体，无菌玻璃器皿和无菌消耗品的功能可靠的高压灭菌器 是我们项目目标成功的关键。该提案寻求一种行政补充，以取代我们 损坏的高压灭菌器具有适合我们实验室的功能性，高质量和高容量的高管 空间。这种新的高压釜将能够满足我们父母赠款的所有灭菌需求，也将 帮助支持我们部门的其他与微生物相关的研究和教学。