Characterization of non-canonical regulatory pathways in the Caulobacter NtrYX signaling system

柄杆菌 NtrYX 信号系统中非典型调控途径的表征

• 批准号: 10794646
• 负责人: Benjamin J Stein
• 金额: $ 5.09万
• 依托单位: UNIVERSITY OF TENNESSEE CHATTANOOGA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-20 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10794646
• 关键词: Administrative Supplement; Agriculture; Bacteria; Biochemical; Caulobacter; Caulobacter crescentus; Complex; Culture Media; Development; Educational process of instructing; Environment; Funding; Future; Genetic; Genetic Transcription; Goals; Health; Homeostasis; Human; Infection; Laboratories; Methods; Microbe; Modeling; Organism; Preparation; Proliferating; Proteins; Regulation; Regulatory Pathway; Research; Signal Pathway; Signal Transduction; Sterility; Sterilization; System; Work; antimicrobial; environmental change; experience; meetings; microbial; parent grant; parent project; success

Project Summary/Abstract All bacteria must sense and respond to internal and external signals to thrive in diverse environments. This sensing is often achieved using protein-based two-component signaling systems. Our funded parent project is developing the Caulobacter crescentus NtrY-NtrX (NtrYX) two-component signaling system as a model for understanding complex bacterial signaling pathways. Our aims seek to understand this atypical two-component signaling system at multiple levels, from regulation of signal flow to changes in transcription. Although our aims employ diverse biochemical and genetic methods, at their core they all require bacterial culture. Thus, a functional and reliable autoclave for the preparation of growth media, sterile glassware, and sterile consumables is key for the success of our project goals. This proposal seeks an administrative supplement to replace our broken autoclave with a functional, high quality, and high-capacity autoclave appropriate for our laboratory space. This new autoclave will be capable of meeting all sterilization demands for our parent grant and will also help support additional microbial-related research and teaching in our department.

项目总结/摘要 所有细菌都必须感知并响应内部和外部信号，才能在不同的环境中茁壮成长。 这种传感通常使用基于蛋白质的双组分信号系统来实现。我们资助的母项目 正在开发新月柄杆菌NtrY-NtrX（NtrYX）双组分信号传导系统，作为 了解复杂的细菌信号通路。我们的目标是试图了解这种非典型的双组分 从信号流的调节到转录的变化，在多个水平上调节信号系统。虽然我们的目标 采用不同的生化和遗传方法，其核心都需要细菌培养。因此 功能可靠的高压灭菌器，用于制备生长培养基、无菌玻璃器皿和无菌耗材 是我们项目目标成功的关键。这项建议旨在以行政补助金取代我们的 破碎的高压灭菌器与功能，高品质，高容量的高压灭菌器适合我们的实验室 空间这种新的高压灭菌器将能够满足我们的母公司赠款的所有灭菌需求， 帮助支持我们部门的其他微生物相关研究和教学。