Characterization of non-canonical regulatory pathways in the Caulobacter NtrYX signaling system

柄杆菌 NtrYX 信号系统中非典型调控途径的表征

• 批准号: 10794646
• 负责人: Benjamin J Stein
• 金额: $ 5.09万
• 依托单位: UNIVERSITY OF TENNESSEE CHATTANOOGA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-20 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10794646
• 关键词: Administrative Supplement; Agriculture; Bacteria; Biochemical; Caulobacter; Caulobacter crescentus; Complex; Culture Media; Development; Educational process of instructing; Environment; Funding; Future; Genetic; Genetic Transcription; Goals; Health; Homeostasis; Human; Infection; Laboratories; Methods; Microbe; Modeling; Organism; Preparation; Proliferating; Proteins; Regulation; Regulatory Pathway; Research; Signal Pathway; Signal Transduction; Sterility; Sterilization; System; Work; antimicrobial; environmental change; experience; meetings; microbial; parent grant; parent project; success

Project Summary/Abstract All bacteria must sense and respond to internal and external signals to thrive in diverse environments. This sensing is often achieved using protein-based two-component signaling systems. Our funded parent project is developing the Caulobacter crescentus NtrY-NtrX (NtrYX) two-component signaling system as a model for understanding complex bacterial signaling pathways. Our aims seek to understand this atypical two-component signaling system at multiple levels, from regulation of signal flow to changes in transcription. Although our aims employ diverse biochemical and genetic methods, at their core they all require bacterial culture. Thus, a functional and reliable autoclave for the preparation of growth media, sterile glassware, and sterile consumables is key for the success of our project goals. This proposal seeks an administrative supplement to replace our broken autoclave with a functional, high quality, and high-capacity autoclave appropriate for our laboratory space. This new autoclave will be capable of meeting all sterilization demands for our parent grant and will also help support additional microbial-related research and teaching in our department.

项目摘要/摘要 所有细菌都必须感知和响应内部和外部信号，才能在不同的环境中茁壮成长。 这种感觉通常是使用基于蛋白质的双组分信号系统来实现的。我们资助的母公司项目 正在开发新月桂杆菌NtrX(NtrYX)双组分信号系统，作为 了解复杂的细菌信号通路。我们的目标是试图理解这种非典型的两个组成部分 多层次的信号系统，从信号流的调节到转录的变化。尽管我们的目标是 使用不同的生化和遗传方法，它们的核心都需要细菌培养。因此，一个 用于制备生长介质、无菌玻璃器皿和无菌耗材的实用可靠的高压灭菌器 是我们项目目标成功的关键。这项提案寻求行政补充，以取代我们的 适用于我们实验室的功能、高质量、高容量的破碎高压灭菌器 太空。这个新的高压灭菌器将能够满足我们母公司赠款的所有灭菌要求，并将 帮助支持我们系其他与微生物相关的研究和教学。