Ultrasensitive quantification of cytokine release from T cells

T 细胞释放细胞因子的超灵敏定量

• 批准号: 10796013
• 负责人: Ying Samuel Hu
• 金额: $ 22.79万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10796013
• 关键词: 3-Dimensional; Acceleration; Goals; Image; Laboratories; Microscope; Parents; Research; System; T-Lymphocyte; cytokine; parent grant; programs; superresolution imaging

PROJECT SUMMARY The limited imaging depth of the current microscopes in the PI’s laboratory poses an obstacle to achieving the overall goal of the parent grant (R35 GM146786). This supplement acquires a single-unit CrestOptics DeepSIM stand-alone system for 3D volumetric superresolution imaging of T cells. The DeepSIM system will substantially expand the experimental capability and accelerate the parent research program.

项目摘要 PI实验室中现有显微镜的成像深度有限，这对实现 父母补助金的总体目标（R35 GM146786）。此补充收购了一个单一的单位CrestOptics DeepSIM独立系统用于T细胞的3D体积超分辨率成像。DeepSIM系统将 大幅度扩大实验能力，加快母体研究计划。

项目成果

Single-Molecule Localization Microscopy Using Time-Lapse Imaging of Single-Antibody Labeling.

使用单抗体标记延时成像的单分子定位显微镜。

• DOI: 10.1002/cpz1.908
• 发表时间: 2023
• 期刊: Current protocols
• 作者: Perera,Thilini;Gunasekara,Hirushi;Hu,YingS
• 通讯作者: Hu,YingS

Superresolution Imaging with Single-Antibody Labeling.

单抗体标记的超分辨率成像。

• DOI: 10.1021/acs.bioconjchem.3c00178
• 发表时间: 2023
• 期刊: Bioconjugate chemistry
• 影响因子: 4.7
• 作者: Gunasekara,Hirushi;Perera,Thilini;Anderson,Jesse;Saed,Badeia;Ramseier,Neal;Keshta,Neama;Hu,YingS
• 通讯作者: Hu,YingS