Imaging Molecular Level Details of Collagen Fibers by VSFG Microscopy

通过 VSFG 显微镜对胶原纤维的分子水平细节进行成像

• 批准号: 10793165
• 负责人: Wei Xiong
• 金额: $ 8.12万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-15 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10793165
• 关键词: Biological; Biological Process; Collagen Fiber; Color; Detection; Development; Electrons; Extracellular Matrix; Image; Imaging Techniques; Measures; Metabolism; Microscope; Microscopy; Molecular; Molecular Evolution; Optics; Research; Resolution; Sampling; Signal Transduction; Time; Tissues; biological systems; biophysical techniques; charge coupled device camera; functional group; molecular imaging; novel strategies; single molecule; spectrograph; success; superresolution imaging; ultra high resolution; vibration

Project Summary I plan to add a single hyperspectral detection unit to a new nonlinear vibrational microscope developed in my lab. This detection unit is composed of a spectrograph and an electron-multiplying charge-coupled device (EMCCD) camera. This unit will enable single-molecule/super-resolution vibrational imaging. We will carry out the research in two stages. The first stage is to verify that the new vibrational imaging technique can detect vibrationally encoded photoluminescent signals of single molecules in standard samples. The second stage uses this single-molecule capacity to image biological systems with a sub- diffraction-limit optical resolution of <300 nm. This new development will revolutionize infrared imaging techniques by enabling unprecedented single-molecule sensitivity. Furthermore, this new single-molecule imaging technique can overcome the issue of limited color channels in photoluminescence (PL) based microscopy. Our long-term vision is to enable new hyperspectral imaging techniques for biophysical and biomedical applications, with unprecedented multiplexing color channels and a new approach of super- resolution imaging that can follow the time evolution of molecular functional groups during metabolism and other biological processes.

项目总结

项目成果

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy.

使用线扫描振动和频发生显微镜的多模态非线性高光谱化学成像。

• DOI: 10.3791/65388
• 发表时间: 2023
• 期刊: Journal of visualized experiments : JoVE
• 作者: Wagner,JacksonC;Yang,Bin;Wu,Zishan;Xiong,Wei
• 通讯作者: Xiong,Wei