Imaging Molecular Level Details of Collagen Fibers by VSFG Microscopy

项目摘要 我计划在年开发的一种新的非线性振动显微镜上增加一个单一的高光谱探测单元。 我的实验室该检测装置由摄谱仪和电子倍增电荷耦合 设备（EMCCD）相机。该单元将实现单分子/超分辨率振动成像。我们 将分两个阶段进行研究。第一阶段是验证新的振动成像 技术可以检测标准中单个分子振动编码的光致发光信号 样品第二阶段使用这种单分子能力来成像生物系统， 衍射极限光学分辨率<300 nm。这一新的发展将彻底改变红外成像 技术，实现前所未有的单分子灵敏度。此外，这种新的单分子 成像技术可以克服基于光致发光（PL）技术的有限颜色通道的问题， 显微镜我们的长期愿景是使新的高光谱成像技术能够用于生物物理和 生物医学应用，具有前所未有的多路复用颜色通道和一种新的方法， 分辨率成像，可以跟踪代谢过程中分子官能团的时间演变 和其他生物过程。

Project Summary I plan to add a single hyperspectral detection unit to a new nonlinear vibrational microscope developed in my lab. This detection unit is composed of a spectrograph and an electron-multiplying charge-coupled device (EMCCD) camera. This unit will enable single-molecule/super-resolution vibrational imaging. We will carry out the research in two stages. The first stage is to verify that the new vibrational imaging technique can detect vibrationally encoded photoluminescent signals of single molecules in standard samples. The second stage uses this single-molecule capacity to image biological systems with a sub- diffraction-limit optical resolution of <300 nm. This new development will revolutionize infrared imaging techniques by enabling unprecedented single-molecule sensitivity. Furthermore, this new single-molecule imaging technique can overcome the issue of limited color channels in photoluminescence (PL) based microscopy. Our long-term vision is to enable new hyperspectral imaging techniques for biophysical and biomedical applications, with unprecedented multiplexing color channels and a new approach of super- resolution imaging that can follow the time evolution of molecular functional groups during metabolism and other biological processes.