Diversity Supplement Carolina Galeano-Naranjo
多样性补充卡罗莱纳·加莱亚诺-纳兰霍
基本信息
- 批准号:10811185
- 负责人:
- 金额:$ 6.72万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2009
- 资助国家:美国
- 起止时间:2009-04-01 至 2025-04-30
- 项目状态:未结题
- 来源:
- 关键词:Automobile DrivingAutophagocytosisBasic Cancer ResearchCancer EtiologyCancer PatientCell Death InductionCell Migration Inhibition functionCell ProliferationCell SurvivalCellsCellular StressCessation of lifeChemoresistanceCollaborationsColon CarcinomaColorectal CancerDevelopmentDiseaseDown-RegulationEIF-2alphaEffectivenessEnsureEpithelial CellsEquilibriumEventFundingGrantHK2 geneInflammationIntegrinsIntestinesKnockout MiceKnowledgeMalignant NeoplasmsMalignant neoplasm of pancreasMediatingMetabolicMetabolismMitochondriaMolecularMutationOncogenicOrganismPH DomainPathway interactionsPatientsPharmaceutical PreparationsPhosphoric Monoester HydrolasesPhosphorylationPhysiologicalPlayProtein DephosphorylationProtein FamilyProtein KinaseProtein phosphataseProto-Oncogene Proteins c-aktRegulationResistanceRoleSignal PathwaySignal TransductionStressSupporting CellTestingTrainingTumor Suppressor ProteinsUnited Statesbiological adaptation to stresscancer cellcancer initiationcancer survivalcancer typecell growth regulationchemotherapycoping mechanismendoplasmic reticulum stressimprovedin vivoinsightleucine-rich repeat proteinmitochondrial autophagymortalitymouse modelmulticatalytic endopeptidase complexnovelprotein expressionresponsesensorsuccesstranslational cancer researchtumor initiationtumor metabolismtumor progressiontumorigenesis
项目摘要
Colorectal cancer is the second leading cause of cancer-related deaths in the United States. Approximately
145,000 new cases and 51,000 deaths are predicted for the year 2019; and this mortality is predominantly due
to poor responses to available treatment options. A better understanding of the molecular events leading to
cancer progression and chemoresistance is needed in order to improve the overall survival of cancer patients.
My lab has been intensively focused on elucidating the role of a novel family of protein phosphatases, PHLPP
(PH domain Leucine-rich-repeats Protein Phosphatase), in inhibiting colon cancer initiation and progression. We
have made substantial progress in understanding the functional importance of PHLPP as a tumor suppressor as
well as the molecular mechanism underlying PHLPP regulation. The overall objective of this study is to further
develop a mechanistic understanding of PHLPP-mediated regulation of cellular stress response in supporting
cell survival and tumorigenesis. In exciting recent findings, we demonstrated that chemotherapy-induced ER
stress promotes PHLPP degradation and PHLPP-loss provides a survival advantage by upregulating
eIF2α/ATF4-mediated signaling. In addition, we found that downregulation of PHLPP promotes mitochondrial
fission by regulating Drp1 phosphorylation. Collectively, the central hypothesis driving this proposed study is that
PHLPP serves an essential stress sensor in CRC, in which cellular stress signals trigger PHLPP degradation to
promote cell survival and tumorigenesis. The following specific aims are proposed: 1) to delineate the molecular
mechanism underlying PHLPP-mediated regulation of eIF2α/ATF4 signaling. We will determine if
downregulation of PHLPP renders colon cancer cells resistant to chemotherapy drugs as a result of autophagy
activation; 2) to determine the functional importance of PHLPP-mediated regulation of mitochondrial dynamics;
and 3) to define the role of mitochondrial dynamics in cooperating with PHLPP-loss to promote tumorigenesis in
vivo. Our study will fill an important knowledge gap on how altered mitochondrial dynamics contributes to tumor
initiation and progression in colon cancer. This supplement will allow Ms. Carolina Galeano-Naranjo to receive
further training in conducting basic and translational cancer research. Her results will assist in determining the
role of PHLPP in CRC by regulating mitochondrial activity. She will determine if PHLPP mutations found in CRC
patients interfer with their ability to control Drp1 phosphorylation and mitochondrial fission. Ultimately, by
providing mechanistic insights into PHLPP-dependent regulation of stress response, our findings will help identify
new treatment options and better predict the effectiveness of chemotherapy agents based on PHLPP status in
cancer patients.
结直肠癌是美国癌症相关死亡的第二大原因。约
预计2019年将有145,000例新病例和51,000例死亡;这一死亡率主要是由于
对现有治疗方案的反应不佳。更好地理解导致
为了提高癌症患者的总体存活率,需要癌症进展和化学抗性。
我的实验室一直致力于阐明一个新的蛋白磷酸酶家族PHLPP的作用
(PH结构域富含亮氨酸重复序列的蛋白磷酸酶),抑制结肠癌的发生和发展。我们
在理解PHLPP作为肿瘤抑制因子的功能重要性方面取得了实质性进展,
以及PHLPP调控的分子机制。本研究的总体目标是进一步
发展PHLPP介导的细胞应激反应调节机制的理解,
细胞存活和肿瘤发生。在最近令人兴奋的发现中,我们证明了化疗诱导的ER
应激促进PHLPP降解,而PHLPP缺失通过上调
eIF 2 α/ATF 4介导的信号传导。此外,我们发现下调PHLPP可以促进线粒体的
通过调节Drp 1磷酸化来分裂。总的来说,推动这项拟议研究的中心假设是,
PHLPP在CRC中充当重要的应激传感器,其中细胞应激信号触发PHLPP降解,
促进细胞存活和肿瘤发生。提出了以下具体目标:1)描绘分子
PHLPP介导的eIF 2 α/ATF 4信号调节的潜在机制。我们将确定是否
PHLPP的下调使结肠癌细胞由于自噬而对化疗药物产生抗性
2)确定PHLPP介导的线粒体动力学调节的功能重要性;
和3)确定线粒体动力学在协同PHLPP丢失促进肿瘤发生中的作用,
vivo.我们的研究将填补一个重要的知识空白,改变线粒体动力学如何有助于肿瘤
结肠癌的发生和发展。这一补充将使卡罗莱纳·加莱亚诺-纳兰霍女士
进一步培训进行基础和转化癌症研究。她的研究结果将有助于确定
PHLPP通过调节线粒体活性在CRC中的作用。她将确定在CRC中是否发现PHLPP突变
患者干扰其控制Drp 1磷酸化和线粒体分裂的能力。最终通过
我们的研究结果将有助于确定PHLPP依赖的应激反应调节机制,
新的治疗选择,并根据PHLPP状态更好地预测化疗药物的有效性,
癌症患者。
项目成果
期刊论文数量(15)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Loss of PHLPP expression in colon cancer: role in proliferation and tumorigenesis.
- DOI:10.1038/onc.2008.450
- 发表时间:2009-02-19
- 期刊:
- 影响因子:8
- 作者:Liu, J.;Weiss, H. L.;Rychahou, P.;Jackson, L. N.;Evers, B. M.;Gao, T.
- 通讯作者:Gao, T.
Inverse agonism at the Na/K-ATPase receptor reverses EMT in prostate cancer cells.
- DOI:10.1002/pros.24144
- 发表时间:2021-07
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Beta-catenin cleavage enhances transcriptional activation.
- DOI:10.1038/s41598-017-18421-8
- 发表时间:2018-01-12
- 期刊:
- 影响因子:4.6
- 作者:Goretsky T;Bradford EM;Ye Q;Lamping OF;Vanagunas T;Moyer MP;Keller PC;Sinh P;Llovet JM;Gao T;She QB;Li L;Barrett TA
- 通讯作者:Barrett TA
The leucine-rich repeat signaling scaffolds Shoc2 and Erbin: cellular mechanism and role in disease.
- DOI:10.1111/febs.15450
- 发表时间:2021-03
- 期刊:
- 影响因子:0
- 作者:Jang H;Stevens P;Gao T;Galperin E
- 通讯作者:Galperin E
Critical role of PI3K/Akt/GSK3β in motoneuron specification from human neural stem cells in response to FGF2 and EGF.
- DOI:10.1371/journal.pone.0023414
- 发表时间:2011
- 期刊:
- 影响因子:3.7
- 作者:Ojeda L;Gao J;Hooten KG;Wang E;Thonhoff JR;Dunn TJ;Gao T;Wu P
- 通讯作者:Wu P
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Tianyan Gao其他文献
Tianyan Gao的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Tianyan Gao', 18)}}的其他基金
Study of PTPRF-Mediated Regulation of Wnt Signaling
PTPRF 介导的 Wnt 信号转导调节研究
- 批准号:
10677248 - 财政年份:2023
- 资助金额:
$ 6.72万 - 项目类别:
Lafora Epilepsy – Basic mechanisms to therapy
拉福拉癫痫 — 治疗的基本机制
- 批准号:
10436430 - 财政年份:2016
- 资助金额:
$ 6.72万 - 项目类别: