SIMULTANEOUS ELECTRICAL AND OPTICAL RECORDINGS IN HEART

描述（改编自申请人的摘要）：该项目将开发 透明电极阵列，可用于测量细胞外 电压和跨膜电压敏感染料荧光 心脏的同一位置 电极将使用以下材料制造 显微镜技术和氧化锡薄膜沉积。 第一个电极阵列将被制造并用于量化 氧化锡薄膜的电学和光学性质是重要的 用于电学和光学测量。 然后将电极阵列 作为原型制造的同时电气和光学 测量. 这些阵列将被用来证明， 可以同时测量心电信号和光信号， 从动脉灌注的兔子心脏的相同位置。 阵列将 用于获取初步数据，以便S. Knisley提交 一个新的R01赠款申请，这将有验证的目标， 常用的细胞外记录方法， 包括阻塞、波前碰撞、再入和多次 颤动的小波 这将有助于解释 细胞外信号，这反过来将允许更好地确定 患者消融术的心律失常起源部位， 允许更好地确定细胞外心律失常的机制 录音. 但首先有必要确定是否有可能 制造电极阵列以测量细胞外电压， 跨膜电压敏感染料荧光同时从 心脏的相同位置。 这个项目有很大的风险， 我不知道锡氧化物之间的界面的电化学性能 心脏组织不知道锡的光学特性 氧化物，他们不知道是否可以把一个阵列结构， 包含所有必要的电学和光学特性。

DESCRIPTION (Adapted from applicant's abstract): This project will develop a transparent electrode array that can be used to measure extracellular voltages and transmembrane voltage-sensitive dye fluorescence simultaneously from the same locations in the heart. Electrodes will be fabricated using microphotolithographic techniques and thin film deposition of tin oxide. The first electrode arrays will be fabricated and used to quantify the electrical and optical properties of the tin oxide film that are important for the electrical and optical measurements. Then electrode arrays will be fabricated as prototypes for the simultaneous electrical and optical measurements. These arrays will be used to demonstrate that the electrodes can simultaneously measure cardiac electrical signals and optical signals from the same locations in arterial-perfused rabbit hearts. The arrays will be used to obtain preliminary data that will allow Dr. S. Knisley to submit a new R01 grant application, which will have the objective of validating the commonly-used extracellular recording method at the sites of arrhythmic conditions including block, wavefront collision, reentry and multiple wavelets of fibrillation. This will improve the interpretation of extracellular signals, which in turn will allow better determination of sites of origins of arrhythmias for ablation procedures in patients and allow better determination of mechanisms of arrhythmias from extracellular recordings. But first it is necessary to determine whether it is possible to fabricate an electrode array to measure extracellular voltages and transmembrane voltage-sensitive dye fluorescence simultaneously from the same locations in the heart. This project has significant risk since they don't know the electrochemical properties of the interface between tin oxide and the heart tissue, they don't know the optical properties of the tin oxide and they don't know if one can put together an array structure that contains all of the necessary electrical and optical properties.