Expression profiling of mouse embryonic and adult stem c

小鼠胚胎和成体干细胞的表达谱

• 批准号: 7325373
• 负责人: Minoru S Ko
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7325373
• 关键词: Expression; profiling; mouse; embryonic; adult

Embryonic stem (ES) cells have the ability to give rise to all cell types, including germ lines (pluripotent), and the ability to undergo an unlimited number of symmetrical divisions without differentiation (self-renewal). For potential therapeutic applications of stem cells, it is paramount to understand how the ES cells maintain their pluripotency and self-renewal, and how the ES cells differentiate into specific cell lineages in vitro. The goal of this research project is to understand the relevant nature of mouse embryonic and tissue stem cells, and to identify genes that are responsible for the maintenance of cellular pluripotency. In our earlier work, we compared the global expression profiles of mouse ES cells and trophoblast stem (TS) cells by DNA microarrays. We studied Esg1, one of the genes identified as a gene expressed specifically in ES cells, and found that the gene encodes an RNA-binding protein that binds to many RNA targets (Tanaka et al., 2006). We have also compared the expression profiles of mouse ES cells undergoing neural differentiation in vitro and those of adult neural stem/progenitor (NS) cells (Aiba et al., 2006). The results suggested that ES cells undergoing neural differentiation in vitro recapitulate the development of neural lineages in vivo. We also found a set of ~4,000 genes, the expression of which increased with neural commitment/differentiation and can be used as a scale for the degree of commitment/differentiation in neural differentiation. We are extending these studies by carrying out global gene expression profiling of mouse embryonic germ (EG) cells and multipotent adult stem cells (MAPCs).

胚胎干细胞（ES）有能力产生所有类型的细胞，包括生殖系（多能性），并有能力进行无限数量的对称分裂而不分化（自我更新）。对于干细胞的潜在治疗应用，了解胚胎干细胞如何维持其多能性和自我更新，以及胚胎干细胞如何在体外分化为特定的细胞系是至关重要的。本研究项目的目标是了解小鼠胚胎和组织干细胞的相关性质，并确定负责维持细胞多能性的基因。在我们早期的工作中，我们通过DNA微阵列比较了小鼠胚胎干细胞和滋养细胞（TS）细胞的全局表达谱。我们对Esg1进行了研究，Esg1是胚胎干细胞中特异性表达的基因之一，并发现该基因编码一种RNA结合蛋白，该蛋白与许多RNA靶标结合（Tanaka et al., 2006）。我们还比较了体外神经分化的小鼠胚胎干细胞和成人神经干细胞/祖细胞的表达谱（Aiba et al., 2006）。结果表明，体外神经分化的胚胎干细胞在体内可以再现神经谱系的发育。我们还发现了一组约4000个基因，这些基因的表达随着神经的承诺/分化而增加，可以作为神经分化的承诺/分化程度的尺度。我们正在通过开展小鼠胚胎生殖细胞（EG）和多能成体干细胞（MAPCs）的全球基因表达谱来扩展这些研究。