Mechanisms of carbon dioxide response in nematodes

线虫二氧化碳反应机制

• 批准号: 8213395
• 负责人: ELISSA ANYON HALLEM
• 金额: $ 24.03万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-01 至 2012-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8213395
• 关键词: Ablation; Acute; Anatomy; Behavioral; Behavioral Assay; Biological Assay; Biological Neural Networks; Caenorhabditis elegans; Calcium; Carbon Dioxide; Cells; Comparative Study; Cryopreservation; Cues; Development; Drug usage; Generations; Genomics; Goals; Head; Health; Human; Image; In Vitro; Insecta; Interneurons; Knowledge; Lasers; Life; Mediating; Modeling; Modification; Molecular Genetics; Morbidity - disease rate; Morphology; Necator americanus; Nematoda; Nematode infections; Nervous System Physiology; Neurobiology; Neurons; Old World Hookworm; Parasites; Parasitic nematode; Phase; Population; Research; Resistance; Risk; Sensory; Signal Pathway; Signaling Molecule; Strongyloides stercoralis; Testing; Time; insight; mortality; neural circuit; positional cloning; prevent; relating to nervous system; research study; response; tool

Parasitic nematodes are a major health concern worldwide, and current strategies for preventing or eliminating nematode infections are insufficient. One possible control strategy is to interfere with the ability of nematodes to locate their hosts. The long-term goal of my research is to better understand how parasitic nematodes locate their hosts, using the free-living nematode C. elegans and the insect-parasitic nematodes Heterorhabditis bacteriophora and Steinernema carpocapsae as models for human-parasitic nematodes. Carbon dioxide is an important host-seeking cue for many parasitic nematodes, yet little is known about the mechanism of CO2 response in nematodes. The overall goal of this proposal is to further our understanding of how parasitic nematodes respond to CO2. I have shown that C. elegans displays acute CO2 avoidance, and I have identified neurons and signaling pathways that are required for this response. I have also found that analogous neurons mediate CO2 attraction in H. bacteriophora. I will now further elucidate the signaling pathways and neural networks that mediate CO2 response in nematodes using molecular, genetic, and neurobiological approaches. I will test the hypothesis that the neural circuits that mediate CO2 response in parasitic nematodes are similar to the neural circuits that mediate CO2 response in free-living nematodes, but contain modifications that reflect the host-seeking requirements of parasites.

寄生线虫是世界范围内的一个主要健康问题，目前的预防或消除策略 线虫感染是不够的。一种可能的控制策略是干扰线虫的能力 来定位它们的宿主我研究的长期目标是更好地了解寄生线虫 利用自由生活的线虫C.线虫和昆虫寄生线虫 Heterorhabditis bacteriophora和Steinernema carpocapsae作为人类寄生线虫的模型。 二氧化碳是许多寄生线虫寻找宿主的重要线索，但对二氧化碳的作用知之甚少。 线虫对CO2的反应机制本提案的总体目标是进一步了解 寄生线虫对二氧化碳的反应我已经证明了C。elegans显示急性CO2回避， 已经确定了这种反应所需的神经元和信号通路。我也发现 类似神经元介导H中的CO2吸引。细菌门。我现在将进一步阐明 利用分子、遗传和神经网络技术， 神经生物学方法。我将测试一个假设，即在大脑中调节二氧化碳反应的神经回路， 寄生线虫与自由生活的线虫介导CO2反应的神经回路相似，但 包含反映寄生虫寻找宿主要求的修改。