DETERMINING THE PATHWAY OF NASCENT-PROTEIN INSERTION THROUGH THE PROTEIN-CONDUC

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心赠款提供。子项目的主要支持 子项目的主要研究者可能是由其他来源提供的， 包括其他NIH来源。 为子项目列出的总成本可能 表示子项目使用的中心基础设施的估计数量， NCRR赠款不直接向子项目或子项目工作人员提供资金。 对于注定要排泄或膜插入的新生蛋白质，核糖体的合成伴随着它们插入到蛋白质传导膜通道SecY中，SecY将蛋白质转位穿过膜或将其插入膜中。与冷冻电子显微镜合作，我们确定了核糖体SecY-nascent-蛋白质中间体的全原子结构，该中间体允许研究膜插入途径。我们试图模拟我们的结构的膜SecY新生蛋白质的一部分，以确定如何揭示的中间体，一个方向反转的N-末端结合SecY侧门外，由于蛋白质的延伸力。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. For nascent proteins destined for excretion or membrane insertion, synthesis by the ribosome is concomitant with their insertion into a protein-conducting membrane channel, SecY, which translocates a protein across or inserts it into the membrane. In collaboration with cryo-electron microscopists we determined an all-atom structure of a ribosome-SecY-nascent-protein intermediate that permits one to investigate the membrane insertion pathway. We seek to simulate the membrane-SecY-nascent protein part of our structure to determine how the intermediate revealed, an orientationally inverted N-terminus binding outside a SecY lateral gate, arises from forces due to protein elongation.