NUCLEAR GENE (XDH) FOR ANGIOSPERM PHYLOGENY
被子植物系统发育的核基因 (XDH)
基本信息
- 批准号:8364215
- 负责人:
- 金额:$ 0.11万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2011
- 资助国家:美国
- 起止时间:2011-09-15 至 2013-07-31
- 项目状态:已结题
- 来源:
- 关键词:AngiospermsBiomedical ResearchComputer AnalysisData SetEvolutionFundingGrantGymnospermsHigh Performance ComputingLengthMethodsModelingNational Center for Research ResourcesNuclearNucleotidesPhylogenyPrincipal InvestigatorResearchResearch InfrastructureResourcesRunningSourceUnited States National Institutes of HealthXDH geneXanthine Dehydrogenasecostprograms
项目摘要
This subproject is one of many research subprojects utilizing the resources
provided by a Center grant funded by NIH/NCRR. Primary support for the subproject
and the subproject's principal investigator may have been provided by other sources,
including other NIH sources. The Total Cost listed for the subproject likely
represents the estimated amount of Center infrastructure utilized by the subproject,
not direct funding provided by the NCRR grant to the subproject or subproject staff.
The nuclear gene xanthine dehydrogenase (Xdh) was sequenced for 247 genera representing all major lineages of angiosperms and gymnosperms and the results need to analyzed using Paup program under likelihood methods.
Approximately 1265 bps were sequenced, resulting in 1557 aligned characters, due to the insertion of gaps. Eleven indels ranging from 3-66 bps in length were inserted in the alignment. Of the aligned characters, 1187 (76%) were variable and 1068 (69%) were potentially parsimony-informative. The ti/tv ratio in Xdh was 1.10, indicating that a high level of saturation was not reached. For likelihood analyses the program MODELTEST v. 3.6 (Posada and Crandall, 1998) was used to select the models of nucleotide evolution and is inserted at the end of the dataset. Dataset has been run on a PC however it is very slow and needs a larger computer for analysis.
这个子项目是利用资源的许多研究子项目之一。
由NIH/NCRR资助的中心拨款提供。对子项目的主要支持
子项目的首席调查员可能是由其他来源提供的,
包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能
表示该子项目使用的中心基础设施的估计数量,
不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。
对被子植物和裸子植物所有主要谱系的247个属的核基因黄嘌呤脱氢酶(Xdh)进行了测序,结果需要用PAUP程序在似然方法下进行分析。
由于插入了间隙,大约1265bps的序列,产生了1557个对齐的字符。在对齐中插入了11个长度从3-66bps不等的INDELs。在对齐的字符中,1187个(76%)是可变的,1068个(69%)是潜在的简约信息。Xdh的ti/tv比为1.10,说明没有达到很高的饱和度。对于可能性分析,使用程序MODELTEST v3.6(Posada和Crandall,1998)来选择核苷酸进化模型,并将其插入到数据集的末尾。数据集已在PC上运行,但运行速度很慢,需要更大的计算机才能进行分析。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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CYNDI MORTON其他文献
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{{ truncateString('CYNDI MORTON', 18)}}的其他基金
NUCLEAR GENE (XDH) FOR ANGIOSPERM PHYLOGENY
被子植物系统发育的核基因 (XDH)
- 批准号:
8171788 - 财政年份:2010
- 资助金额:
$ 0.11万 - 项目类别:
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