Identification of novel transcriptional regulators of lipid metabolism.

转录辅激活因子PGC-1 α在脂质代谢中起着核心作用;尽管其特定的调节功能已被广泛研究，但对可能作为辅调节因子并响应代谢线索开启或关闭其活性的潜在相互作用分子的身份知之甚少。 我们已经采取了生物化学的方法，然后质谱和最近的特点是一种新的PGC-1 α结合分子，能够提高PGC-1 α的体外转录活性。 通过功能获得和丧失实验，我们目前正在评估这种新型辅因子对PGC-1 α调控的基因表达的影响，并确定其对体内脂质β-氧化的总体影响。

The transcriptional coactivator PGC-1alpha plays a central role in lipid metabolism; although its specific regulatory functions have been extensively studied, not much is known about the identity of potential interacting molecules that could act as coregulators and switch on or off its activity in response to metabolic cues. We have undertaken a biochemical approach followed by mass spectrometry and recently characterized a novel PGC-1alpha binding molecule that is able to enhance PGC-1alpha's transcriptional activity in vitro. By gain-and loss-of-function experiments, we are currently assessing the effects of this novel cofactor on the expression of genes regulated by PGC-1alpha and we are determining its overall effects on lipid beta-oxidation in vivo.