UMass Mouse Metabolic Phenotyping Center - Animal Core

项目摘要-摘要 精确的表型鉴定在很大程度上取决于动物受试者的质量。稳定住房和畜牧业 将可能扭曲表型结果的外部因素的影响降至最低。此外，小鼠提交了 到马萨诸塞州大学，MMPC与来自不同微生物来源的队列共享空间。它们和老鼠一样 在居留的群体中，必须防止潜在的外来病原体的交叉感染。这个 动物核心在Jerald Silverman博士的监督下运作，他是核心董事，负责监督 华盛顿大学马萨诸塞州医学院的动物护理设施。人源化小鼠细胞移植及其评价 Sub-Core由Dale Greiner博士执导，他将提供人性化的鼠标和优雅的技术来评估 移植的人胰岛和干细胞来源的β细胞在免疫缺陷小鼠中的体内功能。次核心 将执行标准化评估程序，为移植的人员提供可量化的指标 人类胰岛或干细胞来源的β细胞。动物核心提供与这些相关的设施和服务 以下具体目标下的优先事项：(1)提供稳定、生物遏制的住房、畜牧业和保健 关爱老鼠(2)提供专门的动物设施，用于饲养(AS2-1043和1042)和现场表型鉴定 程序(AS2-1043A)和额外的表型仪器，(3)评估微生物图谱 每个队列并向中心主任提供关于结果的解释和建议，以及(4) 在中心主任酌情决定的情况下，向表型鉴定服务的用户提供结果和建议 提交的小鼠的健康状态。动物核心的目标是支持大学MMPC的运作 与动物护理和小鼠表型鉴定质量相关的问题。

Project Summary - Abstract Precise phenotyping depends significantly on the quality of animal subjects. Stable housing and husbandry minimize the influence of exogenous factors that could distort phenotyping results. Additionally, mice submitted to the UMass MMPC share space with cohorts from microbiologically diverse sources. They, as well as mice housed in resident colonies, must be protected against potential cross-infection by adventitious agents. The Animal Core operates under the supervision of Dr. Jerald Silverman as the Core Director, who oversees the animal care facility of UMass Medical School. The Humanized Mouse Cell Transplantation and Assessment sub-Core is directed by Dr. Dale Greiner who will offer the humanized mice and elegant techniques to assess in vivo function of transplanted human islets and stem cell-derived β-cells in immunodeficient mice. The sub-Core will perform standardized assessment procedures that will provide quantifiable metrics for the transplanted human islets or stem cell-derived β-cells. The Animal Core provides facilities and services relevant to these priorities under the following specific aims: (1) to provide stable, biocontainment housing, husbandry and health care for mice (2) to provide a dedicated animal facility for housing (AS2-1043 and 1042) and on-site phenotyping procedures (AS2-1043A) with additional phenotyping instruments, (3) to assess the microbiological profile of each cohort and provides the Center Director with interpretation and advice regarding the results, and (4) to provide to the users of the phenotyping service, at the discretion of the Center Director, results and advice on the health status of submitted mice. The goal of the Animal Core is to support the operation of UMass MMPC with issues pertinent to animal care and quality of phenotyping mice.