UMass Mouse Metabolic Phenotyping Center - Animal Core

项目摘要-摘要 精确的表型分型在很大程度上取决于动物受试者的质量。稳定的住房和畜牧业 最大限度地减少可能扭曲表型结果的外源因素的影响。此外，提交的小鼠 麻省大学MMPC与来自微生物多样性来源的队列共享空间。它们和老鼠一样 必须保护其免受外来因子的潜在交叉感染。的 动物核心在Jerald Silverman博士的监督下运作，他是核心总监，负责监督 麻省大学医学院的动物护理设施。人源化小鼠细胞移植及其评价 sub-Core由Dale Greiner博士指导，他将提供人源化小鼠和优雅的技术来评估 移植的人胰岛和干细胞衍生的β细胞在免疫缺陷小鼠中的体内功能。的副芯部 将执行标准化的评估程序，为移植的 人胰岛或干细胞衍生的β细胞。动物中心提供与这些相关的设施和服务 在以下具体目标下的优先事项：（1）提供稳定的生物防护住房、畜牧业和健康 护理小鼠（2），为圈养（AS 2 -1043和1042）和现场表型分析提供专用动物设施 程序（AS 2 - 1043 A）与其他表型分析仪器，（3）评估微生物概况 每个队列，并为中心主任提供有关结果的解释和建议，以及（4） 根据中心主任的决定，向表型分析服务的用户提供以下结果和建议： 提交小鼠的健康状况。动物核心的目标是支持马萨诸塞大学MMPC的运作 与动物护理和表型小鼠质量相关的问题。

Project Summary - Abstract Precise phenotyping depends significantly on the quality of animal subjects. Stable housing and husbandry minimize the influence of exogenous factors that could distort phenotyping results. Additionally, mice submitted to the UMass MMPC share space with cohorts from microbiologically diverse sources. They, as well as mice housed in resident colonies, must be protected against potential cross-infection by adventitious agents. The Animal Core operates under the supervision of Dr. Jerald Silverman as the Core Director, who oversees the animal care facility of UMass Medical School. The Humanized Mouse Cell Transplantation and Assessment sub-Core is directed by Dr. Dale Greiner who will offer the humanized mice and elegant techniques to assess in vivo function of transplanted human islets and stem cell-derived β-cells in immunodeficient mice. The sub-Core will perform standardized assessment procedures that will provide quantifiable metrics for the transplanted human islets or stem cell-derived β-cells. The Animal Core provides facilities and services relevant to these priorities under the following specific aims: (1) to provide stable, biocontainment housing, husbandry and health care for mice (2) to provide a dedicated animal facility for housing (AS2-1043 and 1042) and on-site phenotyping procedures (AS2-1043A) with additional phenotyping instruments, (3) to assess the microbiological profile of each cohort and provides the Center Director with interpretation and advice regarding the results, and (4) to provide to the users of the phenotyping service, at the discretion of the Center Director, results and advice on the health status of submitted mice. The goal of the Animal Core is to support the operation of UMass MMPC with issues pertinent to animal care and quality of phenotyping mice.