Liver Histology Core

肝脏组织学核心

• 批准号: 9414644
• 负责人: Gary Kanel
• 金额: $ 11.14万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9414644
• 关键词: Bromodeoxyuridine; CASP3 gene; Cellular biology; Chemicals; Collaborations; Communication; Computer software; Custom; Dehydration; Development; Fibrosis; Frozen Sections; GRP78 gene; Growth; Histologic; Histology; Hospitals; Image; Immunohistochemistry; In Situ Nick-End Labeling; Inflammation; Laboratories; Lasers; Left; Liver; Liver diseases; Medical center; Microdissection; Microscopy; Names; Necrosis; Oils; Paraffin; Pathologist; Peroxidases; Preparation; Publications; Reporting; Research; Research Personnel; Research Support; Services; Slide; Stains; System; Time; Tissue imaging; base; career; cellular imaging; histological specimens; improved; investigator training; member; microscopic imaging; response

ABSTRACT The Liver Histology (LH) Core is a newly-established Core, which has evolved as a result of its splitting from the previous Cell and Tissue Imaging Core, under which it was a subcore (the other being previous Microscopy Subcore). Histology services of RCLD started in 2007 in response to the growing-evolving needs of Center Members and have expanded substantially over time, established an impressive track record of high-quality, rapid turn-around service to our growing user base and remain in continuous high demand. The long-term objectives of Liver Histology Core are the following: 1) Perform routine histology, i.e. regular paraffin processing, sectioning and H&E, Sirius red and Oil red staining and provide assistance in interpretation and quantitation of findings. 2) Cut unstained slides, which the users can then stain themselves. 3) Maintain a deparaffination and dehydration line of chemicals under a hood, accessible to users. 4) Provide frozen sectioning. 5) Add other services, based on the evolving needs of our user base (e.g. due to demand, we have added TUNEL, caspase 3, PCNA, BrdU, Grp78, Cox IV, F4/80, myeloperoxidase and Ki67 stainings). 6) Provide and train investigators in the use of the Core's state-of-the-art Leica LMD7000 laser microdissection system and customized slide preparations. 7) Provide a stand-alone workstation with microscope, image capture camera and processing software for independent and/or assisted use and analyses-interpretation of histology images. 8) Assist investigators in development of immunohistochemistry for specific needs. 9) Assist investigators in interpretation of histological findings and in the development of some quantitative and semi- quantitative systems for scoring findings (e.g. necrosis, inflammation, fibrosis, steatosis).

抽象的 肝脏组织学 (LH) 核心是一个新建立的核心，它是从肝脏组织学核心中分裂出来的结果。 之前的细胞和组织成像核心，其下是一个子核心（另一个是之前的显微镜 子核心）。 RCLD 的组织学服务于 2007 年开始，以满足中心不断增长的需求 随着时间的推移，成员数量大幅增加，建立了令人印象深刻的高质量记录， 为我们不断增长的用户群提供快速周转的服务，并保持持续的高需求。长期来看 肝脏组织学核心的目标如下： 1) 进行常规组织学检查，即常规石蜡 处理、切片和 H&E、天狼星红和油红染色，并提供解释和帮助 研究结果的定量。 2) 剪下未染色的载玻片，然后用户可以自行染色。 3）维持一个 化学物质的脱蜡和脱水线位于引擎盖下，可供用户使用。 4) 提供冷冻 切片。 5) 根据我们用户群不断变化的需求添加其他服务（例如，由于需求，我们有 添加了 TUNEL、caspase 3、PCNA、BrdU、Grp78、Cox IV、F4/80、髓过氧化物酶和 Ki67 染色）。 6） 提供并培训研究人员使用 Core 最先进的 Leica LMD7000 激光显微切割 系统和定制的载玻片制备。 7）提供独立的显微镜、图像工作站 捕获相机和处理软件，用于独立和/或辅助使用和分析-解释 组织学图像。 8) 协助研究人员开发满足特定需求的免疫组织化学。 9) 协助 研究人员解释组织学结果并开发一些定量和半定量方法 对结果进行评分的定量系统（例如坏死、炎症、纤维化、脂肪变性）。