权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Core C: B Cell Core

核心C：B细胞核心

基本信息

批准号：
10731279
负责人：
Kristina De Paris
金额：
$ 26.78万
依托单位：
UNIV OF NORTH CAROLINA CHAPEL HILL
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-06-01 至 2028-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10731279
关键词：
Adult Antibodies Antibody Avidity Antibody Repertoire Antibody Response Antigens Autologous Avidity B-Cell Antigen Receptor B-Cell Development B-Lymphocytes B-cell receptor repertoire sequencing Bar Codes Binding Biological Assay Biometry Bone Marrow Cell Lineage Cells Collaborations Computer Analysis Data Development Dose Engineering Environment Enzyme-Linked Immunosorbent Assay Epitopes Evolution Flow Cytometry Future Generations Goals HIV HIV vaccine HIV-1 Immune Immune response Immune system Immunity Immunization Infant Infection Laboratories Lead Life Lymphoid Tissue Macaca Macaca mulatta Mediating Medicine Messenger RNA Molecular Profiling Monoclonal Antibodies Natural Immunity Nature Phenotype Plasma Plasma Cells Predisposition Process Proteomics Regimen Reproducibility Resources Running Serum Sorting Specificity Surface Plasmon Resonance Technology Time Vaccinated Vaccination Vaccine Design Vaccinee Vaccines Virus Work autoreactivity experience genetic signature genomic platform innovation lymph nodes microbial microbiome microorganism interaction neutralizing antibody next generation sequencing nonhuman primate programs response single-cell RNA sequencing time use tool transcriptomics transmission process vaccine evaluation vaccine immunogenicity vaccine strategy

项目摘要

ABSTRACT – B Cell Core Early development of bNAbs in infants suggest potentially distinct mechanisms in the generation of antibody breadth due to the highly dynamic nature of the infant immune system. Moreover, we have observed elicitation of bNAb precursors in infant rhesus monkeys with germline-targeting HIV Env SOSIP immunization. We hypothesize that induction of bnAb precursor B cells using germline-targeting HIV Env SOSIP immunogens requires specific innate and microbial interactions which are modifiable to enhance future HIV vaccine strategies, particularly in early life. In this context, we propose a B Cell Core that will perform in-depth quantification and characterization of the plasma and B cell responses after BG505 SOSIP GT1.1 immunizations in infant macaques over time through i) the quantification of the functional activity, avidity and target epitope of polyclonal plasma antibody responses (Aim 1) and ii) single cell RNA sequencing of antigen-specific B cell analysis (Aim 2) to evaluate the evolution of vaccine-elicited HIV bNAb precursors and relate these responses to innate immunity (Project 1) and microbial interactions (Project 2). To accomplish this goal, the B Cell Core will draw on the extensive experience and unique resources in antibody and B cell response characterization of the B Cell Core Leader Dr. Marit van Gils at Amsterdam UMC and Dr. Sallie Permar at Weill Cornell Medicine.

摘要：B细胞核心