Estimation and characterization of dysregulated circular RNA in Alzheimers Disease

阿尔茨海默病中失调的环状 RNA 的估计和表征

• 批准号: 10019249
• 负责人: Myriam Gorospe
• 金额: $ 8.11万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10019249
• 关键词: Alzheimer' s Disease; Binding; Brain; Brain region; Collection; Computer software; DNA Sequence Alteration; Data; Data Set; Databases; Digestion; Disease; Exoribonucleases; Family; Family Study; Genetic Transcription; High-Throughput RNA Sequencing; Human; Link; Malignant Neoplasms; Methods; Mutate; Parkinson Disease; Patients; Pattern; Poly(A) Tail; Porifera; RNA; Refractory; Sampling; Testing; age related; bioinformatics tool; circular RNA; deep sequencing; design; genomic tools; mind control; transcriptome sequencing

This project has reached the following results thus far: We have analyzed publicly available, high-throughput RNA sequencing data to identify RNA expression patterns (linear and circular) in AD and control brains. We found in public databases >170 RNA-seq samples from Alzheimers Disease (AD) patients and associated controls. However, to find circular RNAs we needed samples which were not selected for RNAs with poly(A) tail. Therefore, in the end only 47 samples (22 controls and 25 AD) from three separate studies from three different brain regions were adequate . We are currently testing if the circRNAs found to change with AD (AIM 1) are also found to be mutated in AD families studied in LNG (AIM 3). RNA will be prepared from brains (normal and AD) from LBN collections or obtained following advice from LBN to validate the circular RNAs identified (AIM 2).

到目前为止，该项目取得了以下成果： 我们分析了公开的高通量RNA测序数据，以确定AD和对照脑中的RNA表达模式（线性和环状）。 我们在公共数据库中发现了来自阿尔茨海默病（AD）患者和相关对照的>170个RNA-seq样品。 然而，为了找到环状RNA，我们需要未选择具有poly（A）尾的RNA的样品。 因此，最终，来自三个不同脑区的三项独立研究的47个样本（22个对照和25个AD）是足够的。 我们目前正在测试发现随AD（AIM 1）变化的circRNA是否也发现在LNG（AIM 3）中研究的AD家族中发生突变。 将从LBN收集的脑（正常和AD）中制备RNA，或根据LBN的建议获得RNA，以验证鉴定的环状RNA（AIM 2）。