Microbiome analysis in a mouse model of Alzheimers Disease (AD)

阿尔茨海默病 (AD) 小鼠模型中的微生物组分析

• 批准号: 10019246
• 负责人: Myriam Gorospe
• 金额: $ 13.52万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10019246
• 关键词: Affect; Aging; Alzheimer' s Disease; Alzheimer' s disease model; Brain; Chronic; Communities; DNA; Elderly; Genome; Goals; Health; Human; Immune system; Inflammaging; Inflammatory; Intestinal permeability; Mus; Patients; Prevotella; Process; Protocols documentation; Ribosomal RNA; Sampling; Serum; Shapes; Standardization; bacterial community; cytokine; gut microbiome; interest; metagenome; microbial; microbiome; microbiome analysis; microbiota; mouse model

We have investigated microbiota community involved in mouse and human fecal samples and recently started working on the serum samples. The most abundant bacterial taxa identified in mouse fecal samples were Bacteroidales, Clostridiales, Lactobacillales, Verrucomicrobiales. Also, there was significant difference in the microbial abundance between 2 month and 48 old mice. We also analyzed 18 human fecal samples from BLSA and found the genus Prevotella was significantly lower in aging (AIM 1a,b) (LGG, TGB, LEPS). We are actively working on standardizing the protocol extract and sequence from CSF and brain in mice (AIM 1c). We are also currently working on the impact of gut microbiome on pro-inflammatory cytokines and intestinal permeability (AIM 2).

我们已经研究了小鼠和人类粪便样本中涉及的微生物群，最近开始研究血清样本。 在小鼠粪便样品中鉴定的最丰富的细菌分类群是拟杆菌目、梭菌目、乳杆菌目、疣微生物目。 2月龄和48日龄小鼠肠道微生物丰度差异显著。 我们还分析了来自BLSA的18份人粪便样品，发现普雷沃菌属在老化（AIM 1a，B）（LGG，TGB，LEPS）中显著较低。 我们正在积极致力于标准化小鼠CSF和脑（AIM 1c）的方案提取物和序列。 我们目前还在研究肠道微生物组对促炎细胞因子和肠道通透性的影响（AIM 2）。