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STUDIES TO EVALUATE THE POTENTIAL FOR ENVIRONMENTAL&THERAPEUTIC AGENTS TO INDUCE IMMUNOTOXICITY - In vitro

评估环境潜力的研究

基本信息

批准号：
10023468
负责人：
FLORENCE BURLESON
金额：
$ 101.79万
依托单位：
BRT-BURLESON RESEARCH TECHNOLOGIES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-08-13 至 2020-08-12
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10023468
关键词：
Animals Autoimmune Diseases Biological Biological Assay Cell Proliferation Chemical Agents Chemicals Communicable Diseases Contracts Dermal Development Echinacea purpurea Endotoxins Event Exposure to Food Additives Formulation Health Health Hazards Hematopoietic Human Hypersensitivity Immunologic Techniques Immunologics Immunosuppression Immunotoxicology In Vitro Industry Collaboration Interagency Coordinating Committee on the Validation of Alternative Methods Measures Methods Molecular National Toxicology Program Neoplasms Peripheral Blood Lymphocyte Pharmaceutical Preparations Poly-fluoroalkyl substances Predictive Value Publishing Reporting Research Resistance Skin Structure Testing Therapeutic Agents United States Environmental Protection Agency Validation Xenobiotics cytokine dietary supplements environmental agent exposed human population immune function immune system function immunoregulation immunotoxicity in vitro Assay in vitro Model in vivo programs respiratory response

项目摘要

Significant focus is being placed on the utility of in vitro models to assess immunomodulation. In vitro cytokine release assays were used to evaluate the ability of 18 perfluoroalkyl substances to modulate cell proliferation and cytokine secretion in human peripheral blood lymphocytes. In vitro cytokine release assays were also used to screen for biological activity in several batches of echinacea purpurea. Results from the in vitro screen and endotoxin assessments were used to select a test article for in vivo studies. We have partnered with the Interagency Coordination Committee on the Validation of Alternative methods and are using recently published combinations of non-proprietary in vitro assays to evaluate the potential for a variety of chemicals to induce skin sensitization. Nominations were received from multiple ICCVAM agency partners and industry collaborators. Approximately 200 compounds are being screened in multiple assays. Draft reports for studies on formulations received through an industry collaboration and Isothiozolinone compounds nominated by the Environmental Protection agency have been received, reviewed and are undergoing finalization.

重要的重点是放在体外模型的效用，以评估免疫调节。体外细胞因子释放试验用于评估18种全氟烷基物质调节人外周血淋巴细胞增殖和细胞因子分泌的能力。体外细胞因子释放测定也用于筛选几批紫锥菊的生物活性。使用体外筛选和内毒素评估的结果选择供试品进行体内研究。我们已与替代方法验证机构间协调委员会合作，并正在使用最近公布的非专有体外试验组合来评估各种化学品诱导皮肤致敏的潜力。提名来自多个ICCVAM机构合作伙伴和行业合作伙伴。大约200种化合物正在多项试验中进行筛选。已收到、审查并正在最后确定关于通过工业合作收到的制剂和环境保护局提名的异噻唑啉酮化合物的研究报告草稿。