P30 - Core Grant for Vision Research

摘要 整体 视觉研究核心补助金的目的是提供支援和设施， 不能通过个人赠款获得，专门用于视觉研究和NEI资助的项目， 贝勒医学院（Baylor College of Medicine）核心还提供空间、支助人员和设备支助， 促进国家的最先进的视觉研究和视觉研究实验室之间的合作在学院。 视觉研究中心目前有24名视觉研究调查员，携带19名合格的NEI RO 1、1个NEI DP 2、1个NEI R21、2个NEI T32、1个NEI U24和7个来自其他NIH研究所的NIH RO 1，及其 研究领域涵盖整个视觉系统的结构、功能和发病机制，从角膜和 透镜到视网膜和中央视觉中心。他们的研究学科包括生物化学，分子生物学， 遗传学、电生理学、基因组/生物信息学分析、心理物理学、计算视觉神经科学 和发育生物学。这些研究人员组成了一个充满活力和高度互动的视觉研究小组， 在过去的5年里，发表了360多篇论文，这些论文都受益于这种使用。 视觉核心在这个竞争性的更新应用程序中，我们报告了我们过去非常成功和富有成效的使用情况 的核心设施，并寻求继续支持四个资源/服务模块：显微镜，数字 成像和组织学模块、仪器模块、小动物视觉系统分析模块 以及基因组学和生物信息学模块。这些模块包含价值超过700万美元的设备 与眼科系和学院基金。愿景核心的具体目标是：（1）提供 扫描共聚焦显微镜和电子显微镜服务，用于高分辨率三维成像 用于荧光染料标记的组织和细胞以及用于眼和神经组织的超微结构分析， 并为共聚焦显微镜和电子显微镜提供组织学设施、服务和培训。(2)到 为Vision Core提供内部定制设计的机器、电子、光学和计算机服务 需要特殊仪器的调查人员，这些仪器在市场上是买不到的。(3)以方便进入 结构、生理、生化和行为测试设施，如OCT、ERG、膜片钳 记录法、水迷宫法、高效液相色谱法、质谱法和OKR法研究小鼠、大鼠和兔的视觉系统 功能、功能障碍和结构/遗传缺陷。(4)提供最先进的基因组技术， 通过利用以下机构的专业知识、技术和资源，为Vision Core实验室提供关键的生物信息学支持： 美国生物技术研究所人类基因组测序中心。(5)促进各种机构之间的互动和合作， 视觉研究小组通过共享使用和核心设施的培训。(6)提供丰富的， 充满活力的研究环境，以招募和留住优秀的视觉研究教师，学生和 博士后研究员;并促进新的和创新的视觉研究项目和计划的启动。

ABSTRACT Overall The objective of the Core Grant for Vision Research is to provide support and facilities, which are not available and cannot be obtained through individual grants, exclusively for vision research and NEI-funded projects at Baylor College of Medicine (BCM). The Core also provides space, support personnel and equipment support to facilitate state-of-the-art vision research and collaborations among vision research laboratories in the College. The Vision Research Core at BCM currently has 24 vision research investigators, carrying 19 eligible NEI RO1s, 1 NEI DP2, 1 NEI R21, 2 NEI T32s, 1 NEI U24 and 7 NIH RO1s from other NIH institutes, and their research areas cover the structure, function and pathogenesis of the entire visual system, from the cornea and lens to the retina and to the central visual centers. Their research disciplines include biochemistry, molecular genetics, electrophysiology, genomic/bioinformatic analysis, psychophysics, computational visual neuroscience and developmental biology. These investigators form a vibrant and highly interactive vision research group, and have published more than 360 papers in the past 5 years, which have benefited from the usage of this Vision Core. In this competing renewal application, we report our highly successful and productive past usage of Core facilities, and seek continued support for four Resource/Service Modules: the Microscopy, Digital Imaging and Histology Module, the Instrumentation Module, the Small Animal Visual System Analysis Module and the Genomics and Bioinformatics Module. These modules contain equipment worth over $7M purchased with Department of Ophthalmology and College funds. The Specific Aims of the Vision Core are: (1) To provide scanning confocal microscope and electron microscope services for high-resolution three-dimensional imaging of tissues and cells labeled with fluorescent dyes and for ultrastructural analysis of ocular and neural tissues, and to provide histological facilities, services and training for both confocal and electron microscopy. (2) To provide in-house, custom-designed machine, electronic, optics and computer services to the Vision Core investigators who need special apparatuses that are not commercially available. (3) To provide access to structural, physiological, biochemical, and behavior testing facilities, such as OCT, ERG, patch clamp recording, water-maze, HPLC, mass spectrometry and OKR, to study mouse, rat and rabbit visual system function, dysfunction, and structural/genetic defects. (4) To provide state-of-the-art genomic technologies and critical bioinformatics support to Vision Core labs by leveraging the expertise, technologies, and resources of the BCM Human Genome Sequencing Center. (5) To foster interactions and collaborations among various vision research groups at BCM through shared use and training of Core facilities. (6) To provide a rich and vibrant research environment to recruit and retain outstanding vision research faculty, students and postdoctoral fellows; and to facilitate initiation of new and innovative vision research projects and programs.