Defining Breath VOC Biomarkers to Improve Respiratory Health of Exposed Veterans

定义呼吸 VOC 生物标志物以改善接触过的退伍军人的呼吸健康

• 批准号: 10015032
• 负责人: Richart W Harper
• 金额: --
• 依托单位: VA NORTHERN CALIFORNIA HEALTH CARE SYS
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10015032
• 关键词: Address; Adverse effects; Afghanistan; Air Pollutants; Alloys; Area; Asia; Asthma; Biological Markers; Breath Tests; Bronchiolitis; California; Cell Culture Techniques; Cells; Chronic Obstructive Airway Disease; Clinic; Clinical Research; Data; Development; Devices; Disease; Disease Management; Down-Regulation; Ecology; Eicosanoids; Engineering; Enrollment; Environmental Exposure; Epithelial; Epithelial Cells; Equipment and supply inventories; Exhalation; Exposure to; Fire - disasters; Funding; Goals; Health; Healthcare; Healthcare Systems; Human; In Vitro; Inflammatory; Iraq; Laboratories; Lung; Lung diseases; Mass Spectrum Analysis; Mathematics; Measures; Mediating; Medicine; Metals; Methods; Military Personnel; Monitor; Newly Diagnosed; Oils; Particulate; Particulate Matter; Pathway interactions; Patients; Population; Positioning Attribute; Prevention; Recording of previous events; Research; Research Personnel; Respiratory Signs and Symptoms; Risk; Sampling; Site; Soldier; Source; System; Technology; Testing; Time; Tracheobronchial; Up-Regulation; Veterans; active duty; airway epithelium; asthma prevention; asthmatic; asthmatic patient; biobank; biomarker discovery; biomarker validation; burn pit; cohort; combustion product; constriction; disease phenotype; epidemiology study; experimental study; human tissue; improved; in vivo; metabolomics; novel; personalized medicine; portability; portable monitoring; pulmonary function; research clinical testing; research study; respiratory health; sensor; tool; toxicant; volatile organic compound

The goal of this resubmitted application is to define signature exhaled breath volatile organic compounds (VOCs) in veterans exposed to toxicants and to develop an effective portable exhaled breath health monitoring system for soldiers at risk of developing respiratory disease. This project will bring a suite of portable sensors forward into use with the aim of supporting large scale epidemiological studies in military respiratory health. Specifically, our proposal responds to the call for research on the causes, treatment and prevention of chronic obstructive pulmonary disease, including identification and validation of biomarkers and disease phenotypes, as well as employing personalized medicine approaches in clinical research and disease management. While environmental triggers have long been recognized as key factors in respiratory disease, it has been challenging to concurrently measure health metrics and environmental exposures and associate the two. Also, a technology gap has existed for portable monitoring systems that can perform these functions simultaneously while continuously logging data. Breath testing of exhaled VOC biomarkers is a new concept that has the potential to transform healthcare for military and civilian populations in the US. Our overarching hypothesis is that a miniature breath analysis sensor can measure signatures of exhaled breath VOCs in real-time and correlate this to ambient field exposures as well as changes in lung function among exposed soldiers. This will allow doctors and researchers to define field exposures that have adverse effects on respiratory health, including worsening of asthma and the development of COPD. In aim #1, we propose to define VOC biomarkers produced by the lung upon exposure to the toxicants. We have developed a reliable method to measure the exceptionally small amount of VOCs emitted from well- differentiated tracheobronchial epithelial (TBE) cells in vitro. We will utilize this system to identify the signature and cellular source of VOCs emitted from TBEs exposed to toxicants and traffic related air pollutants. These experiments will be performed in primary cells from the lungs of humans in order to correlate with in vivo studies. In aim#2, we plan to expand our inventory of portable breath samplers for the clinical studies. In aim #3, we will perform a short-term, one- week clinical study in 4 cohorts at the VANCHCS and UC Davis sites to define a signature of breath biomarkers, including eicosanoids, that correlate with the presence of disease, namely asthma. A total of 100 veteran and civilian subjects will be enrolled over 4 years. Together, data from our tools will allow military doctors, researchers and others to more fully understand asthma and other respiratory diseases in exposed field soldiers in ways that has never been possible before.

这个重新提交的申请的目标是定义呼出气体挥发性有机物的特征