Remote Modulation of the Peripheral Nervous System
Remote Modulation of the Peripheral Nervous System
批准号:
10002786
负责人:
Sarah Amy Stanley
金额:
$13.0万
依托单位国家:
美国
项目类别:
财政年份:
2017
资助国家:
美国
项目状态:
已结题
起止时间:
2017-08-24 至 2021-07-31
关键词:
Afferent NeuronsAnimalsAreaBiologicalBiomedical EngineeringCalciumCationsCellsCharacteristicsChimeric ProteinsChloridesDiabetes MellitusDiseaseElectromagnetic FieldsElectromagneticsElectrophysiology (science)Enterobacteria phage P1 Cre recombinaseExcisionFOS geneFerritinGene DeliveryGenesImageImmunohistochemistryImplantInternal Ribosome Entry SiteIon ChannelIonsIslets of LangerhansKineticsLigandsLightMagnetismMalignant NeoplasmsMethodsModificationMotionMusNerveNeural InhibitionNeural PathwaysNeuronsObesityOptical MethodsOrganPancreasPancreatic HormonesPeripheralPeripheral NervesPeripheral Nervous SystemPhysiologicalPopulationProteinsRegulationRoleRouteSensorySignal TransductionStructureSystemTRPV1 geneTechnologyTemperatureTestingTimeTissuesTranslationsVanilloidViralViral VectorViruscell typeclinical applicationglucose tolerancein vivoiron oxide nanoparticlemagnetic fieldnanoparticlenerve supplyneuroregulationneurotransmissionnovelnovel therapeutic interventionpromoterradio frequencyreceptorrelating to nervous systemresponsetool
中文摘要
项目总结
英文摘要
Project Summary
Modulating the activity of peripheral nerves innervating specific organs and defined cell types within these
organs will help us understand the relationship between neural signals and organ function. We propose to
develop and validate the use of non-invasive neural modulation in the peripheral nervous system in vivo. Many
tools can be used for temporal regulation of neural activity in the CNS, from light activated channels to
designer receptors but these are not universally applicable, particularly in the periphery. Optical methods
require permanent implants which may be difficult to fix or even cause damage in peripheral tissues. In
addition, these tools only activate local neural populations in a small portion of an organ. In contrast, designer
receptors and their ligands can target neurons across a larger area but have a relatively slow time course.
Neural modulation with radiowaves or magnetic fields allows remote, rapid activation or inhibition of neural
activity across an entire organ.
We have recently shown that a distinctive combination of non-invasive radiowave and magnetic field signals,
biological ferritin nanoparticles and bioengineered ion channels can be used to remotely activate and inhibit
CNS neural activity in freely moving animals. Targeted neurons express genetically encoded nanoparticles
tethered to a modified ion channel, transient receptor potential vanilloid 1, TRPV1. Radiowaves or magnetic
fields freely penetrate tissue to heat and/or move the nanoparticle and activate TRPV1. Modifications of
TRPV1 allow either neural activation or silencing. We will now develop and validate tools for non-invasive
activation and silencing of peripheral nerves using viral vectors applicable to several species and we will
demonstrate their utility by modulating innervation of the endocrine pancreas. Specifically, we will develop,
validate and characterize 1) tools for remote activation and inhibition of neurons innervating the pancreas using
viruses with retrograde spread and neuron-specific expression of activating or inhibitory constructs and 2) tools
for remote activation and inhibition of specific neural pathways (parasympathetic, sympathetic and sensory)
innervating the pancreas.
We will develop a range of tools for remote modulation of parasympathetic, sympathetic and sensory
peripheral nerves innervating an organ. These tools will be broadly applicable and extend the methods
available to investigate the physiological roles of peripheral nerves in regulating organ function.
期刊论文(4)
专著(0)
科研奖励(0)
会议论文
Mapping and specific viral targeting of peripheral pancreatic innervation.
周围胰腺神经支配的绘图和特异性病毒靶向。
DOI:
--
发表时间:
2022
期刊:
FASEB journal : official publication of the Federation of American Societies for Experimental Biology
影响因子:
--
作者:
[JimenezGonzalez,Maria, Li,Rosemary, Pomeranz,LisaE, Alvarsson,Alexandra, Marongiu,Roberta, Hampton,RollieF, Vasavada,RupangiC, Schwartz,GaryJ, Stanley,SarahA]
通讯作者:
Stanley,SarahA
DOI:
10.21769/bioprotoc.4103
发表时间:
2021-08
期刊:
Bio-protocol
影响因子:
0.8
作者:
[A. Alvarsson;M. Jimenez-Gonzalez;Rosemary Li;Carolina Rosselot;N. Tzavaras;Zhuhao Wu;S. Stanley]
通讯作者:
A. Alvarsson;M. Jimenez-Gonzalez;Rosemary Li;Carolina Rosselot;N. Tzavaras;Zhuhao Wu;S. Stanley
DOI:
10.1038/s41598-020-70067-1
发表时间:
2020-08-04
期刊:
SCIENTIFIC REPORTS
影响因子:
4.6
作者:
[Brier, Matthew, I, Mundell, Jordan W., Dordick, Jonathan S.]
通讯作者:
Dordick, Jonathan S.
Neural control of pancreatic endocrine function in obesity and diabetes
-
批准号:10326394
-
项目类别:
-
资助金额:$59.5万
-
财政年份:2021
-
负责人:Sarah Amy Stanley
-
依托单位:
Neural control of pancreatic endocrine function in obesity and diabetes
-
批准号:10542366
-
项目类别:
-
资助金额:$59.5万
-
财政年份:2021
-
负责人:Sarah Amy Stanley
-
依托单位:
Collaborative Research: Elucidating the Mechanism of Magnetogenetics for Remote Activation of Cell Function
-
批准号:1930157
-
项目类别:Standard Grant
-
资助金额:$23.16万
-
财政年份:2019
-
负责人:Sarah Amy Stanley
-
依托单位:
Remote Modulation of the Peripheral Nervous System
-
批准号:9415871
-
项目类别:
-
资助金额:$50.0万
-
财政年份:2017
-
负责人:Sarah Amy Stanley
-
依托单位:
Remote regulation of neural activity
-
批准号:8821955
-
项目类别:
-
资助金额:$41.95万
-
财政年份:2014
-
负责人:Sarah Amy Stanley
-
依托单位:
海外基金