Core 1 - Research Technology Core

摘要 在狼疮发病机制的这个程序中，我们试图了解驱动自身免疫的途径。的 研究技术核心（核心1）致力于为这些B细胞的研究提供标准化的手段。 和抗体反应，并用于研究宿主与肠道中的细菌的关系。 核心项目将根据每个项目的需要提供定制服务。通过 集中这些设施，我们将提供服务，提供规模经济和效率。这些 方法是最先进的，否则无法从纽约大学核心设施或商业 厂商 研究技术核心将在四个领域进行已经验证的测定： i）来自血清和粪便样品的自身抗体调查。 ii）通过内源性产生的伊加分离肠中包被的粪便细菌。 iii）通过下一代测序的16 S rRNA方法进行微生物组分析。 iv）大规模BCR转录物库文库。 在此，我们将支持所有三个项目的研究，这些研究涉及来自以下组织的特异性抗体/B细胞克隆的贡献： 免疫耐受的初始破坏，以及从临床前疾病向明显临床疾病的过渡，以及 以自身免疫性疾病的自我延续为特征的正在进行的疾病过程的后期阶段。

ABSTRACT In this program on lupus pathogenesis, we seek to understand the pathways that drive autoimmunity. The Research Technology Core (Core 1) is dedicated to providing standardized means for the study of these B cell and antibody responses, and for investigations of the host relationship with commensal bacteria in the gut. The Core will provide services that have customized to the needs of each of the Projects. Through centralization of these facilities, we will provide services that provide economies of scale and efficiency. These methods are state-of-the-art and are otherwise not available from NYU Core facilities or from commercial vendors. The Research Technology Core will perform already validated assays in four areas: i) Autoantibody surveys from serum and fecal samples. ii) Isolation of fecal bacteria coated in the bowel by endogenously produced IgA. iii) Microbiome analyses by 16S rRNA methods by Next Generation Sequencing. iv) Large-scale BCR transcript repertoire libraries. Herein, we will support studies for all three Projects of the contributions of specific antibody/B-cell clones from the initial breach of immune tolerance, and in the transition from preclinical to overt clinical disease, and during the later phase of ongoing disease process characterized by self-perpetuation of autoimmune disease.