Tissue Modeling Core

组织建模核心

• 批准号: 10006104
• 负责人: Hernan Alcides Flores-Rozas
• 金额: $ 12.94万
• 依托单位: FLORIDA AGRICULTURAL AND MECHANICAL UNIV
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-19 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10006104
• 关键词: Address; Archives; Area; Basic Science; Benign; Biocompatible Materials; California; Cancer Burden; Cancer Center; Cell Culture Techniques; Cell Line; Clinical; Clinical Research; Collaborations; Collection; Complex; Comprehensive Cancer Center; Conscious; County; DNA; Data; Development; Disease; Education; Ensure; Epidemiology; Evaluation; Florida; Funding; Future; Genetic; Genomic DNA; Goals; Growth; Health Services Accessibility; Human; In Vitro; Infrastructure; Institutes; Institution; International; Latino; Life Style; Link; Los Angeles; Malignant neoplasm of pancreas; Malignant neoplasm of prostate; Medical Research; Medical center; Mission; Mitochondria; Normal tissue morphology; Oncologist; Outcome; Pancreas; Pancreatectomy; Pathologic; Patients; Pharmacologic Substance; Pharmacy facility; Pilot Projects; Plasma; Population; Procedures; Process; RNA; Race; Radiation Oncology; Research; Research Personnel; Residual state; Resources; Sampling; Science; Services; Specimen; Technology; Testing; Therapeutic; Tissue Banks; Tissue Model; Tissue Procurements; Tissue Sample; Tissues; Toxicology; Transference; Translational Research; Tumor Tissue; Tumor-Derived; Universities; Xenograft procedure; anticancer research; base; biobank; blood product; cancer health disparity; cancer type; clinical database; college; comorbidity; drug discovery; ethnic diversity; experience; gemcitabine; genomic data; health equity; improved; mid-career faculty; neoplasm registry; neoplasm resource; pancreatic cancer patients; pancreatic neoplasm; patient oriented; patient population; population based; racial diversity; recruit; repository; response; skills; success; three-dimensional modeling; transcriptome sequencing; transcriptomics; tumor; virtual

PROJECT ABSTRACT: TISSUE MODELING CORE The long-term goal of the Tissue Modeling Core (TMC) is to provide a growing archive of fixed and living tissues, DNA, and RNA for sequencing that will support the Florida-California CaRE2 Health Equity Center’s mission. The TMC will develop a robust, well-annotated biorepository of tissue samples from a racially and ethnically diverse Florida and California patient population and make them available to the CaRE2 Center investigators. The short-term goals of the TMC are to provide the necessary tissue samples for the three projects while preparing for the future needs of the existing and upcoming CaRE2 projects. The TMC will address the challenge of recruiting Blacks and Latinos in medical research and the limited supply of quality biospecimens and genomic data for these populations. The TMC Specific Aims are to: Aim 1. Develop a virtual repository that will provide access to biospecimens from diverse tumor repositories through a single access point provided by the CaRE2 Center. Many cancer types have been linked to outcome disparities, with the causes of disparities including genetics, access to care, comorbidities, and lifestyle. Aim 1A: Tissue and biomaterials collection. The established tissue-modeling repository will comprise: (1) the UF CTSI tissue repositories; and (2) the USC Residual Tissue Repository linked to the California Cancer Registry and Norris Comprehensive Cancer Center/Los Angeles County Medical Center patient samples. The immediate tasks of the TMC include: (1) Full Project 1: provision of tissue, plasma, serum, and RNA needed for evaluation of mitochondrial peptidomics and transcriptomics of prostate cancer; (2) Full Project 2: provision of tissue and RNA of pancreatic cancers to understand gemcitabine efficacy in pancreatic cancer; and (3) Pilot Project 3: provision of normal pancreas tissues and sequencing for understanding pancreatic cancer development. Aim 1B: Infrastructure transfer to FAMU. The TMC will also seek to expand the FAMU infrastructure for fixed and living tissues. It will leverage that growth using the cooperating CTSIs, Cancer Centers, and the OneFlorida Clinical Research Consortium. Aim 2. Living tissue and living tumor tissue acquisition. The immediate tasks of the TMC include: (1) Full Project 2: provision of pancreatic cancer PDXs and paired cell lines for 2D and 3D modeling; and (2) Pilot Project 3: provision of normal living pancreas tissues from patients undergoing pancreatic surgery for benign disease. By achieving these Specific Aims, the TMC will: (1) enhance cancer disparities science in the important areas of tissue-modeling research; (2) efficiently expand cancer disparities resources for CaRE2 researchers by leveraging the expertise, services, and facilities in Florida and California, and (3) ensure a sustained focus on transferring know-how, technology, and collaborations with FAMU.

项目摘要：组织建模核心 组织建模核心 (TMC) 的长期目标是提供不断增长的固定和活体档案 用于测序的组织、DNA 和 RNA，这将支持佛罗里达-加利福尼亚州 CaRE2 健康公平中心的 使命。 TMC 将开发一个强大的、注释明确的生物样本库，其中包含来自不同种族和不同种族的组织样本。 佛罗里达州和加利福尼亚州不同种族的患者群体，并将他们提供给 CaRE2 中心 调查人员。 TMC的短期目标是为三个项目提供必要的组织样本 同时为现有和即将到来的 CaRE2 项目的未来需求做好准备。 TMC 将解决 在医学研究中招募黑人和拉丁裔面临的挑战以及优质生物样本的供应有限 以及这些人群的基因组数据。 TMC 的具体目标是： 目标 1. 开发一个虚拟存储库，提供对来自不同肿瘤的生物样本的访问 通过 CaRE2 中心提供的单一访问点访问存储库。许多癌症类型已被 与结果差异相关，差异的原因包括遗传、获得护理的机会、合并症、 和生活方式。目标 1A：组织和生物材料收集。建立的组织模型存储库将 包括：（1）UF CTSI组织库； (2) 链接到的 USC 残留组织存储库 加州癌症登记处和诺里斯综合癌症中心/洛杉矶县医疗中心 患者样本。 TMC 的近期任务包括： (1) 完整项目 1：提供组织、血浆、血清、 评估前列腺癌线粒体肽组学和转录组学所需的 RNA； (2) 满 项目2：提供胰腺癌的组织和RNA，以了解吉西他滨对胰腺癌的疗效 癌症; (3) 试点项目3：提供正常胰腺组织并测序以供理解 胰腺癌的发展。目标 1B：向 FAMU 转移基础设施。 TMC 还将寻求扩大 用于固定和活体组织的 FAMU 基础设施。它将通过合作 CTSI 来利用这种增长， 癌症中心和 OneFlorida 临床研究联盟。 目标 2. 活体组织和活体肿瘤组织采集。 TMC 的当前任务包括： (1) 完整项目2：提供胰腺癌PDX和用于2D和3D建模的配对细胞系；和（2） 试点项目3：为接受胰腺手术的患者提供正常的活体胰腺组织 良性疾病。 通过实现这些具体目标，TMC 将：(1) 加强重要领域的癌症差异科学 组织建模研究领域； (2) 通过以下方式有效扩大 CaRE2 研究人员的癌症差异资源 利用佛罗里达州和加利福尼亚州的专业知识、服务和设施，以及 (3) 确保持续关注 与 FAMU 转让专业知识、技术和合作。