Developing Heparan Sulfate Glycan Array

开发硫酸乙酰肝素聚糖阵列

• 批准号: 10005531
• 负责人: Guowei Su
• 金额: $ 144.37万
• 依托单位: GLYCAN THERAPEUTICS CORPORATION
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10005531
• 关键词: Academia; Address; Applications Grants; Awareness; Bacterial Infections; Binding; Bioinformatics; Biological; Biological Process; Blood coagulation; Communities; Computer software; Data; Development; Discipline; Embryonic Development; Extracellular Matrix; Funding; Grant; Heparitin Sulfate; Individual; Inflammatory Response; Internet; Label; Libraries; Literature; Measures; Methods; Microarray Analysis; Modeling; Oligosaccharides; Outcome; Paper; Pattern; Pharmacologic Substance; Phase; Physiological; Play; Polysaccharides; Preparation; Process; Proteins; Publishing; Research; Research Personnel; Role; Services; Small Business Innovation Research Grant; Small Business Technology Transfer Research; Structure; Sulfate; Surface; Techniques; Therapeutic; Triage; Viral; Visualization software; base; biological research; computerized tools; density; design; innovation; interest; phase 1 study; phase 2 study; rapid growth; success; tool; web site

This SBIR phase II proposal is aimed at developing microarray technology to study the interactions between heparan sulfate (HS) and proteins. HS is a highly sulfated polysaccharide displaying a wide range of biological functions. There is a strong demand in the research community for a high throughput method to study the binding between HS and proteins. During the previously funded phase I study (R41GM123792), Glycan Therapeutics has developed and launched a pilot HS microarray service. The outcome from the STTR grant clearly demonstrates that the research community greatly appreciates a convenient and reliable HS microarray service. This phase II application is focused on the expansion of the HS microarray service. Aim 1 is designed to expand the HS oligosaccharide library from 95 to 250 structures. A larger number of HS oligosaccharides on the microarray will enhance its ability to study the relationship between the function and structure of HS saccharide sequences. Aim 2 will develop a bioinformatic approach to structurally triage the oligosaccharides based on microarray data. The bioinformatic method is expected to predict additional structures that may interact with the target proteins. A streamlined website will simplify data presentation for end users to acquire the data without technical complexity. Our success will lead to a convenient and reliable research tool for advancing glycosciences.

该 SBIR 第二阶段提案旨在开发微阵列技术来研究 硫酸乙酰肝素（HS）和蛋白质之间的相互作用。 HS是一种高度硫酸化的 多糖具有广泛的生物学功能。有一种强烈的 研究界对高通量方法来研究结合的需求 HS 和蛋白质之间。在之前资助的第一阶段研究期间 （R41GM123792），Glycan Therapeutics 开发并推出了试点 HS 微阵列服务。 STTR 拨款的结果清楚地表明 研究界非常赞赏方便可靠的 HS 微阵列 服务。该二期应用的重点是 HS 微阵列的扩展 服务。 目标 1 旨在将 HS 寡糖库从 95 个结构扩展至 250 个结构。 微阵列上更多的 HS 寡糖将增强其能力 研究HS糖序列的功能和结构之间的关系。 目标 2 将开发一种生物信息学方法来对寡糖进行结构分类 基于微阵列数据。生物信息学方法有望预测更多 可能与目标蛋白相互作用的结构。精简的网站将变得简单 数据呈现，让最终用户无需复杂的技术即可获取数据。我们的 成功将为推进提供方便可靠的研究工具 糖科学。