Controlled Delivery of Plasmid DNA via Low-Temperature Ion Deposition
通过低温离子沉积控制质粒 DNA 的传递
基本信息
- 批准号:10075665
- 负责人:
- 金额:$ 42.24万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-01-08 至 2022-05-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The long-range goal of this research program is the development of efficient in vivo gene delivery systems.
The goal of this specific project is the development of an improved minimally invasive system for the delivery of
plasmid DNA to the skin. Skin is easily accessibility which makes it an excellent target for gene therapy
applications whether it is for directly treating cutaneous diseases or utilizing the skin as a depot for delivering
proteins directly to the circulation for systemic therapy. To take advantage of the easy accessibility of the skin
it is critical to develop non-contact approaches that are a simple and direct in vivo method to deliver DNA and
can be accomplished in a minimally invasive way. We have been working on developing such approaches and
previously developed devices and protocols that utilized electrotransfer for this delivery. While these devices
have worked effectively and can accomplish this in a relatively non-invasive manner, it is still necessary to
have contact between the electrodes and the tissue target. In addition, the applied voltages needed to achieve
delivery on some occasions may cause cellular or tissue damage or potential discomfort. It is critical to
develop an alternative approach that can work as well as electrotransfer but do it without the contact. An
additional consideration is to develop an approach and/or device that will allow for better control of delivery and
move towards a more predictable and reproducible pattern of expression. To accomplish this, we propose to
utilize a non-thermal atmospheric pressure plasma device that can permeabilize cells and facilitate plasmid
DNA uptake. We hypothesize that delivery is achieved by ion deposition on the surface of the target
tissue and that if the level of ion deposition is regulated then expression levels can be controlled. The
novel plasma device to be further developed and evaluated is based on nanosecond pulsed air plasmas. Using
this approach will allow us to develop a small portable device that could be battery operated. This will be a
non-contact delivery device that will minimize or eliminate potential discomfort and/or cellular damage. The
following specific aims will be performed as part of this project. 1) To evaluate non-thermal atmospheric
plasma devices for controlled production of ions that can be deposited on tissue surface; 2) To evaluate NTAP
devices producing various level of ions for delivery of plasmid DNA to the skin and to determine the duration of
maximal expression levels and to determine if this time can be increased by performing multiple delivery
procedures; and 3) to determine if the system established in the first two aims can deliver plasmids encoding
therapeutic proteins. The investigators have extensive expereince in developing non-thermal plasma devices
and gene transfer so are well suited to successfully complete the study.
该研究计划的长期目标是开发高效的体内基因传递系统。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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RICHARD HELLER其他文献
RICHARD HELLER的其他文献
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{{ truncateString('RICHARD HELLER', 18)}}的其他基金
Identification of impedance measurement devices, heating hardware, and operating parameters to augment instrumentation for a commercial in vivo electroporation system
识别阻抗测量装置、加热硬件和操作参数,以增强商业体内电穿孔系统的仪器
- 批准号:
10484502 - 财政年份:2022
- 资助金额:
$ 42.24万 - 项目类别:
Controlled Delivery of Plasmid DNA via Low-Temperature Ion Deposition
通过低温离子沉积控制质粒 DNA 的传递
- 批准号:
9447306 - 财政年份:2018
- 资助金额:
$ 42.24万 - 项目类别:
Thermal Assisted Gene Electro Transfer to the Skin
热辅助基因电转移至皮肤
- 批准号:
9230219 - 财政年份:2015
- 资助金额:
$ 42.24万 - 项目类别:
Efficient Delivery of Plasmid DNA to Achieve Appropriate Transgene Expression
高效递送质粒 DNA 以实现适当的转基因表达
- 批准号:
9248339 - 财政年份:2015
- 资助金额:
$ 42.24万 - 项目类别:
Efficient Delivery of Plasmid DNA to Achieve Appropriate Transgene Expression
高效递送质粒 DNA 以实现适当的转基因表达
- 批准号:
9040116 - 财政年份:2015
- 资助金额:
$ 42.24万 - 项目类别:
Efficient Delivery of Plasmid DNA to Achieve Appropriate Transgene Expression
高效递送质粒 DNA 以实现适当的转基因表达
- 批准号:
8817124 - 财政年份:2015
- 资助金额:
$ 42.24万 - 项目类别:
Efficient Delivery of Plasmid DNA to Achieve Appropriate Transgene Expression
高效递送质粒 DNA 以实现适当的转基因表达
- 批准号:
10063643 - 财政年份:2015
- 资助金额:
$ 42.24万 - 项目类别:
Thermal Assisted Gene Electro Transfer to the Skin
热辅助基因电转移至皮肤
- 批准号:
8887520 - 财政年份:2015
- 资助金额:
$ 42.24万 - 项目类别:
Therapeutic Potential of IL-15 Plasmid Delivery to Tumors Using Electroporation
使用电穿孔将 IL-15 质粒递送至肿瘤的治疗潜力
- 批准号:
7765169 - 财政年份:2008
- 资助金额:
$ 42.24万 - 项目类别:
Therapeutic Potential of IL-15 Plasmid Delivery to Tumors Using Electroporation
使用电穿孔将 IL-15 质粒递送至肿瘤的治疗潜力
- 批准号:
7769844 - 财政年份:2008
- 资助金额:
$ 42.24万 - 项目类别:
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