Single Cell Spatial Analysis in Tissue

组织中的单细胞空间分析

• 批准号: 10044479
• 负责人: Jared Kyle Burks
• 金额: $ 25.84万
• 依托单位: UNIVERSITY OF TX MD ANDERSON CAN CTR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-09 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10044479
• 关键词: Biological Assay; Biological Sciences; Cancer Biology; Cancer Center; Cancer Center Support Grant; Cell Communication; Cell Separation; Cells; Collaborations; Complex; Comprehensive Cancer Center; Consultations; Core Facility; Cytometry; Education; Ensure; Equipment; Experimental Designs; Faculty; Flow Cytometry; Growth; Hour; Image; Immune; Industry; Methodology; Microscope; Modeling; Process; Proteomics; Publications; Reporting; Research; Research Personnel; Services; Source; Standardization; Suspensions; Technical Expertise; Technology; Testing; Tissues; Training; University of Texas M D Anderson Cancer Center; anticancer research; cancer genomics; cellular imaging; flexibility; imaging facilities; imaging system; instrumentation; liquid crystal polymer; member; neoplastic cell; new technology; novel; programs; single cell technology; stem; success; tool

Project Summary/Abstract Flow Cytometry, Mass Cytometry, Cell Sorting, and Imaging are essential tools for a wide range of methodologies aimed at understanding complex single cell proteomic and genomic cancer research. The instrumentation involved is large, complex, and can be difficult to maintain and operate. As such, a targeted core facility is critical for a Comprehensive Cancer Center such as MD Anderson. The FCCIF, est. 1982, provides these services. Since the last CCSG renewal (2012) 404 cancer center members from all 16 cancer center programs have used the facility and produced 604 publications. Many of the hot topics in cancer biology currently stem from immune cell interaction with the tumor cell; immunephenotyping is an in demand field of research that requiring a unique high plex assay. This requires a specialized imaging or cytometry approach and the FCCIF has specifically acquired two different platforms because of their diversity and flexibility. These platforms are the Vectra Multispectral imaging system and the CyTOF (suspension and imaging) Mass Cytometers. The FCCIF provides a common source of state-of-the-art expertise and technical skills to support the varied research efforts of MD Anderson investigators. The FCCIF provides a comprehensive service to all of the institutional investigators regardless of prior expertise. Dr. Jared K. Burks (author of this R50, co-Director of the FCCIF-NC) provides critical services including: 1) education, 2) consultation on needed technological approach, 3) training on equipment use, analytics, and proper experimental design. I identify and acquire novel technologies for use by MDACC investigators and I provide support in a manner to ensure success and mitigate pitfalls. I am expected to develop new strategies and technologies to meet the research needs of our faculty. A few examples of what I have currently been working on is the Vectra Polaris Multispectral Microscope, Helios and Hyperion Suspension and Imaging CyTOFs. The Polaris has operated on average 13.5 hours per day for the last year. The CyTOFs are reporting 5090% growth since the last CCSG renewal. These results stem from mitigating the pitfalls, making the technologies approachable and affordable, and supporting the researchers throughout the process all the way to publication. Collaborations, both academic and industry, have resulted to develop or test novel applications. The models we have created to support these technologies have and will continue to be shared with others operating the technologies. We are highly involved with both developing and standardizing new technologies for use at MD Anderson and with our facilities and researchers implementing them worldwide.

项目总结/摘要 流式细胞仪、质谱仪、细胞分选仪和成像仪是各种方法学的基本工具 旨在了解复杂的单细胞蛋白质组学和基因组癌症研究。仪表 所涉及的是大的、复杂的，并且可能难以维护和操作。因此，有针对性的核心设施至关重要 比如MD安德森这样的综合癌症中心。FCCIF，est. 1982年，提供这些服务。 自上一次CCSG更新（2012年）以来，来自所有16个癌症中心项目的404名癌症中心成员使用了 出版了604本出版物。目前癌症生物学中的许多热门话题都源于免疫 细胞与肿瘤细胞相互作用;免疫表型分析是一个需要独特的研究领域， 高倍数测定。这需要专门的成像或细胞计数方法，FCCIF专门 由于其多样性和灵活性，我们收购了两个不同的平台。这些平台是Vectra 多光谱成像系统和CyTOF（悬浮和成像）质谱细胞仪。FCCIF提供 国家的最先进的专业知识和技术技能的共同来源，以支持MD的各种研究工作 安德森调查员。FCCIF为所有机构调查人员提供全面的服务 不管之前的专业知识。Jared K.博士伯克斯（R50的作者，FCCIF-NC的联合主任）提供了 关键服务包括：1）教育，2）所需技术方法的咨询，3） 设备使用、分析和适当的实验设计。我发现并获取新技术， MDACC调查人员和我以确保成功并减少陷阱的方式提供支持。我被期待着 开发新的策略和技术，以满足我们的教师的研究需求。举几个 我目前正在研究的是Vectra Polaris多光谱显微镜，Helios和Helios Suspension 和成像细胞因子。北极星去年平均每天运行13.5小时。CyTOF 自上一次CCSG续订以来，增长了5090%。这些结果源于减少陷阱， 技术平易近人，价格实惠，并在整个过程中支持研究人员， 出版的方式。学术界和工业界的合作导致了新产品的开发或测试 应用.我们为支持这些技术而创建的模型已经并将继续被共享 与其他人操作的技术。我们高度参与开发和标准化新的 MD安德森使用的技术，以及我们的设施和研究人员在全球范围内实施这些技术。