Neuronal Physiology Core

神经生理学核心 摘要： 本计划项目中提出的研究将确定 后脑神经回路，是肠-脑交流的基础，并确定 在这个回路中发生，以响应减肥手术。探讨其功能与调控 在细胞水平上，在这个回路中不同的神经元群体，神经元生理学 核心将进行专门的细胞功能测定，有针对性地选择神经元 使用遗传编码工具结合独特的遗传小鼠模型进行亚型研究。这些 测定包括1.体内纤维光度法测量来自遗传编码的钙信号 钙指示剂（GECI）表达后脑神经元群体，作为细胞的读数 活动响应刺激，前和后VSG。2.离体电生理分析 基因标记和识别的神经元，以直接评估潜在的细胞机制 垂直袖状胃切除术（VSG）前后胃功能的变化及调节 光遗传学和离体电生理学，以评估定义的 神经元群体4.体内光遗传学刺激或不同遗传学的抑制 靶向神经元群体，以评估其对生理学的调节的影响。 过程（例如进食行为、条件性位置偏好）

Neuronal Physiology Core Abstract: The studies proposed in this Program Project will define the structure, function and regulation of hindbrain neural circuitry that underlies gut-brain communication and ascertain alterations that occur within this circuitry in response to bariatric surgery. To probe the function and regulation of distinct neuronal populations within this circuitry, at the cellular level, the Neuronal Physiology Core will conduct specialized assays of cellular function that are targeted to select neuronal subtypes using genetically encoded tools combined with unique genetic mouse models. These assays include 1. in vivo fiber photometry to measure calcium signals from genetically encoded calcium indicator (GECI)-expressing hindbrain neuronal populations, as a readout of cellular activity in response to stimuli, pre and post-VSG. 2. ex vivo electrophysiologial analysis of genetically-labeled and indentified neurons to directly assess cellular mechanisms underlying function and regulation pre and post vertical sleeve gastrectomy (VSG) 3.combined optogenetics and ex vivo electrophysiology to assess the functional connectivity of defined neuronal populations 4. in vivo optogenetic stimulation or inhibition of distinct genetically targeted neuronal populations to assess the impact of their modulation on physiologic processes (e.g. feeding behavior, conditioned place preference)