Neuronal Physiology Core

神经生理学核心 摘要： 本计划项目中提出的研究将确定 作为肠脑沟通基础的后脑神经回路，并确定 发生在这个回路内，以响应减肥手术。探求其功能与规律 在细胞水平上，在这个回路中的不同神经元群体，神经生理学 CORE将进行专门的细胞功能分析，以选择神经元 使用遗传编码工具结合独特的遗传老鼠模型的亚型。这些 检测方法包括1.体内纤维光度法测量基因编码的钙信号。 钙指示剂(GECI)-表达后脑神经元群，作为细胞读数 VSG前后对刺激反应的活动。2.离体电生理分析 用基因标记和识别的神经元直接评估潜在的细胞机制 垂直袖式胃切除术(VSG)前后的功能与调节3.联合 光遗传学和体外电生理学评估Defined的功能连接性 神经元种群4.体内不同基因的光遗传刺激或抑制 靶向神经元群体以评估其调节对生理的影响 过程(例如，进食行为、条件化的地点偏好)

Neuronal Physiology Core Abstract: The studies proposed in this Program Project will define the structure, function and regulation of hindbrain neural circuitry that underlies gut-brain communication and ascertain alterations that occur within this circuitry in response to bariatric surgery. To probe the function and regulation of distinct neuronal populations within this circuitry, at the cellular level, the Neuronal Physiology Core will conduct specialized assays of cellular function that are targeted to select neuronal subtypes using genetically encoded tools combined with unique genetic mouse models. These assays include 1. in vivo fiber photometry to measure calcium signals from genetically encoded calcium indicator (GECI)-expressing hindbrain neuronal populations, as a readout of cellular activity in response to stimuli, pre and post-VSG. 2. ex vivo electrophysiologial analysis of genetically-labeled and indentified neurons to directly assess cellular mechanisms underlying function and regulation pre and post vertical sleeve gastrectomy (VSG) 3.combined optogenetics and ex vivo electrophysiology to assess the functional connectivity of defined neuronal populations 4. in vivo optogenetic stimulation or inhibition of distinct genetically targeted neuronal populations to assess the impact of their modulation on physiologic processes (e.g. feeding behavior, conditioned place preference)