Neuronal Physiology Core
神经生理学核心
基本信息
- 批准号:10263949
- 负责人:
- 金额:$ 39.86万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-09-20 至 2024-06-30
- 项目状态:已结题
- 来源:
- 关键词:Advisory CommitteesAnimalsBiological AssayBody WeightBrainCalciumCalcium SignalingCell Culture TechniquesCell modelCell physiologyCellsCellular AssayCollaborationsCommunicationComplementComputer softwareConsultationsCustomDataData AnalysesData CollectionDoctor of PhilosophyElectrophysiology (science)EnsureEquipmentExperimental DesignsFeeding behaviorsFiberFood Intake RegulationGastrectomyGastrointestinal tract structureGeneticGenetic ModelsHuman ResourcesHypothalamic structureImageImaging TechniquesIndividualIonsLabelLaboratoriesMeasurementMeasuresMetabolic DiseasesMethodologyMichiganMonitorNeuronsNoiseOutputPhotometryPhysiologicalPhysiological ProcessesPhysiologyPlayPopulationPostdoctoral FellowPreparationProcessProtocols documentationQuality ControlRegulationReproducibilityResearch DesignResearch Project GrantsRodentRoleSample SizeSecureServicesSliceStandardizationStimulusStructureStudentsSupervisionTechniquesTechnologyTestingTrainingUniversitiesWorkbariatric surgerycalcium indicatorconditioned place preferenceelectrical measurementexperienceexperimental studyextracellularfluorophoregut-brain axishindbrainin vivointerestmeetingsmouse modelneural circuitneuronal circuitrynoveloperationoptogeneticspatch clampprogramsrelating to nervous systemresponsetheoriestool
项目摘要
Neuronal Physiology Core
Abstract:
The studies proposed in this Program Project will define the structure, function and regulation of
hindbrain neural circuitry that underlies gut-brain communication and ascertain alterations that
occur within this circuitry in response to bariatric surgery. To probe the function and regulation
of distinct neuronal populations within this circuitry, at the cellular level, the Neuronal Physiology
Core will conduct specialized assays of cellular function that are targeted to select neuronal
subtypes using genetically encoded tools combined with unique genetic mouse models. These
assays include 1. in vivo fiber photometry to measure calcium signals from genetically encoded
calcium indicator (GECI)-expressing hindbrain neuronal populations, as a readout of cellular
activity in response to stimuli, pre and post-VSG. 2. ex vivo electrophysiologial analysis of
genetically-labeled and indentified neurons to directly assess cellular mechanisms underlying
function and regulation pre and post vertical sleeve gastrectomy (VSG) 3.combined
optogenetics and ex vivo electrophysiology to assess the functional connectivity of defined
neuronal populations 4. in vivo optogenetic stimulation or inhibition of distinct genetically
targeted neuronal populations to assess the impact of their modulation on physiologic
processes (e.g. feeding behavior, conditioned place preference)
神经生理学核心
摘要:
本计划项目中提出的研究将确定
后脑神经回路,是肠-脑交流的基础,并确定
在这个回路中发生,以响应减肥手术。探讨其功能与调控
在细胞水平上,在这个回路中不同的神经元群体,神经元生理学
核心将进行专门的细胞功能测定,有针对性地选择神经元
使用遗传编码工具结合独特的遗传小鼠模型进行亚型研究。这些
分析包括1.体内纤维光度法测量来自遗传编码的钙信号
钙指示剂(GECI)表达后脑神经元群体,作为细胞的读数
活动响应刺激,前和后VSG。2.离体电生理分析
基因标记和识别的神经元,以直接评估细胞机制,
垂直袖状胃切除术(VSG)前后胃功能的变化及调节
光遗传学和离体电生理学,以评估定义的
神经元群体4.体内光遗传学刺激或不同遗传学的抑制
靶向神经元群体,以评估其对生理学的调节的影响。
过程(例如进食行为、条件性位置偏好)
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Paulette B. Goforth其他文献
Paulette B. Goforth的其他文献
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{{ truncateString('Paulette B. Goforth', 18)}}的其他基金
Defining roles for area postrema neuron cell types in food intake and nausea
定义后区神经元细胞类型在食物摄入和恶心中的作用
- 批准号:
10582220 - 财政年份:2023
- 资助金额:
$ 39.86万 - 项目类别:
Dysfunctional Orexin-Regulated Neural Circuits in Alzheimer's Disease
阿尔茨海默病中食欲素调节的神经回路功能失调
- 批准号:
10571896 - 财政年份:2022
- 资助金额:
$ 39.86万 - 项目类别:
Dysfunctional Orexin-Regulated Neural Circuits in Alzheimer's Disease
阿尔茨海默病中食欲素调节的神经回路功能失调
- 批准号:
10372270 - 财政年份:2022
- 资助金额:
$ 39.86万 - 项目类别:
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