Improved Ratiometric Voltage-Sensitive Dyes for In-Vitro and In-Vivo Imaging of Brain Electrical Signaling

改进的比例电压敏感染料用于脑电信号的体外和体内成像

• 批准号: 10018113
• 负责人: Corey Acker
• 金额: $ 14.1万
• 依托单位: POTENTIOMETRIC PROBES, LLC
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-13 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10018113
• 关键词: Acute; Address; Adoption; Area; BRAIN initiative; Back; Biological Assay; Biomedical Engineering; Brain; Brain imaging; Cardiac; Cell Culture Techniques; Cells; Cellular Assay; Chemicals; Communication; Communities; Computer Models; Data; Development; Disease; Dyes; Family suidae; Fluorescent Dyes; Fluorine; Funding; Goals; Heart; Heart Research; Histocompatibility; Image; Imaging Techniques; In Vitro; Lead; Light; Link; Market Research; Measurable; Measurement; Microscope; Mission; Morphologic artifacts; Motion; Neurons; Neurosciences; Neurosciences Research; Noise; Optics; Pathologic; Performance; Phase; Photons; Preparation; Process; Reaction Time; Reporting; Research; Research Personnel; Sampling; Schizophrenia; Services; Signal Transduction; Slice; Structure; Synapses; Technology; Testing; Time; TimeLine; Translating; autism spectrum disorder; brain cell; brain tissue; commercialization; design; experience; experimental study; high throughput screening; imaging modality; improved; in vitro testing; in vivo; in vivo imaging; light intensity; live cell imaging; nervous system disorder; optogenetics; ratiometric; sensor; tool; two-photon; usability; virtual; voltage; voltage sensitive dye; web site

Project Summary/Abstract Potentiometric Probes Voltage-sensitive dyes can be applied to brain cells and tissue to study the electrical signals used by the brain to function. The fluorescent dye molecules light up under a microscope and convert tiny electrical signals to measurable changes in light intensity. Virtually all neurological disorders involve changes in the brain’s normal electrical activity and can be studied with voltage-sensitive dyes. The electrochromic voltage-sensitive dyes developed by the founders of Potentiometric Probes have extremely fast response times, one of several features that make them ideal for studying spiking in the brain’s neuronal networks. However, electrochromic dyes typically suffer from a lack of sensitivity making them difficult to use outside specialized labs. Potentiometric Probes looks to overcome this weakness with new dyes and new imaging techniques that can boost the sensitivity and information conveyed in voltage-imaging experiments. New in-vitro and in-house live cell test assays along with computational modeling will allow better optimization of voltage-sensitivity for recording neuronal spiking with the highest possible signal-to-noise. Fast, electrochromic dyes are compatible with ratiometric imaging, which has important applications for in-vivo recordings. If successful, availability of improved, better validated dyes would make voltage imaging accessible to a much wider body of researchers and provide them with a more powerful tool for studying the electrical signals underlying normal and pathological brain function.

项目摘要/抽象电位测量探针 电压敏感的染料可以应用于脑细胞和组织，以研究大脑使用的电信号 功能。荧光染料分子在显微镜下照明，然后将微小的电信号转换为 光强度的可测量变化。几乎所有神经系统疾病都涉及大脑正常的变化 电活动，可以用对电压敏感的染料进行研究。电化电压敏感染料 电位计量探针的创始人开发的响应时间非常快，这是几个 使其成为研究大脑神经元网络中尖峰的理想特征。但是，电致色素 染料通常缺乏敏感性，因此难以在专业实验室外部使用。 电位计量探针希望通过新染料和新成像技术克服这种弱点 提高电压成像实验中传达的灵敏度和信息。新的室内和内部现场直播 细胞测试测定以及计算建模将使电压敏感性更好地优化 记录具有最高可能的信号到噪声的神经元尖峰。快速，电致染色器兼容 使用比率成像，它在体内记录中具有重要的应用。如果成功，可用性 改进，更好地验证的染料将使大量研究人员访问电压成像 并为他们提供更强大的工具，用于研究正常的电信号，并 病理大脑功能。