Shared Resource-Mass Spectrometry Core-Proteomics and Metabolomics

质谱学核心：蛋白质组学和代谢组学共享资源-项目摘要/摘要 自上次更新以来，质谱学核心：蛋白质组学和代谢组学显著 升级，包括一名新主任和工作人员，购买最先进的仪器，扩大 核心蛋白质组学服务，以及新陈代谢组学服务的引入。通过改进每一个方面 ，质谱学核心为癌症中心成员提供最高级别的支持 他们的蛋白质组、代谢组学和定制的多肽合成需求。主任和协理主任 提供有关设计、实施和分析广泛的 实验包括：蛋白质鉴定、蛋白质定量、翻译后鉴定 修饰、分泌蛋白质/肽分析、非靶向脂组分析、靶向脂组学和 代谢组学、胆汁酸分析、氨基酸定量、脂肪酸分析和定制多肽合成。 近年来，公司收购了五台新的质谱仪，并拥有一支经验丰富的核心员工队伍， 在广泛的蛋白质组学和代谢组学应用方面具有丰富的工作知识，已被招募到 监督这个设施。工作人员提供知识和技术技能，以充分利用这一点 精密的新设备。核心员工正在不断努力实施新的分析技术 这些快速发展的领域，同时支持研究人员进行数据分析以及手稿和赠款 准备工作。多肽合成服务最近已整合到此核心中，并提供定制 为多肽的合成、高效液相色谱纯化和基于质谱学的分析提供服务，包括Long 多肽合成和定制修饰。这一核心被绝大多数癌症中心广泛使用 成员和工作人员继续致力于会议和推进他们的研究。The Core努力做到 通过以下方式促进癌症研究：1)为发现提供复杂的仪器：核心具有五个高 分辨率质谱仪(三个用于蛋白质组学，两个用于代谢组学)和一个多肽合成器， 2)提供专业知识：核心工作人员数十年的综合经验可随时获取 Salk调查人员就实验设计、数据获取和分析进行咨询，以及 手稿和赠款准备，3)提供关键服务：核心工作人员提供可复制的、高质量的 代谢和蛋白质组分析前的高质量样品制备服务，以及提供定制 多肽合成和纯化，4)提供教育、培训和支持：各级Salk研究人员 可以接受成功应用于蛋白质组和蛋白质组的质谱学方面的培训 代谢组学分析，特别是关于实验设计和数据分析，以及5)确定 和评估新技术：核心寻求确保它不断提供最先进的 仪器和经过验证的技术进步，以增强核心用户的发现 实验。

Mass Spectrometry Core: Proteomics and Metabolomics Shared Resource - Project Summary/Abstract Since the last renewal, the Mass Spectrometry Core: Proteomics and Metabolomics has been significantly upgraded, including a new Director and staff, the purchase of state-of-the art instrumentation, expansion of Core proteomics services, and the introduction of metabolomics services. By improving every aspect of service, the Mass Spectrometry Core provides Cancer Center members with the highest level of support for their proteomic, metabolomic, and custom peptide synthesis needs. The Director and Associate Director provide expert consultation concerning the design, implementation, and analysis of a wide range of experiments including: protein identification, protein quantification, identification of post-translational modifications, secreted protein/peptide analysis, untargeted lipidomics profiling, targeted lipidomics and metabolomics, bile acids analysis, amino acid quantitation, fatty acid analysis, and custom peptide synthesis. In recent years, five new mass spectrometers have been acquired and a highly experienced Core staff, with strong working knowledge of a wide range of proteomics and metabolomics applications, has been recruited to oversee this facility. The staff provides the knowledge and technical skills to get the most out of this sophisticated new equipment. The Core staff are constantly working to implement new analytical techniques in these rapidly evolving fields while supporting researchers with data analysis, as well as manuscript and grant preparation. Peptide synthesis services have recently been consolidated into this Core and offer custom services for synthesis, HPLC purification, and mass spectrometry-based analysis of peptides, including long peptide syntheses and custom modifications. This Core is broadly used by the vast majority of Cancer Center members, and the staff remain dedicated to meeting and advancing their research. The Core strives to facilitate cancer research by: 1) providing sophisticated instrumentation for discovery: the Core has five high- resolution mass spectrometers (three for proteomics and two for metabolomics) and one peptide synthesizer, 2) providing expertise: decades of combined experience from the Core staff are made readily accessible to Salk investigators for consultations concerning experimental design, data acquisition and analysis, and manuscript and grant preparation, 3) providing critical services: The Core staff provides reproducible, high- quality sample preparation services prior to metabolic and proteomic analysis, as well as providing custom peptide synthesis and purification, 4) providing education, training, and support: Salk researchers at all levels of expertise can receive training in the successful applications of mass spectrometry for proteomic and metabolomics analysis, particularly with respect to experimental design and data analysis, and 5) identifying and evaluating new technologies: the Core seeks to ensure that it continuously offers state-of-the-art instrumentation and validated technological advances to enhance discovery as a result of the Core users' experiments.