Shared Resource-Mass Spectrometry Core-Proteomics and Metabolomics

共享资源-质谱核心-蛋白质组学和代谢组学

• 批准号: 10114242
• 负责人: Alan Saghatelian
• 金额: $ 29.29万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-12-31 至 2024-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10114242
• 关键词: Amino Acids; Bile Acids; Cancer Center; Cancer Center Support Grant; Consultations; Custom; Data Analyses; Ensure; Equipment; Experimental Designs; Fatty Acids; Grant; High Pressure Liquid Chromatography; Institutes; Investments; Knowledge; Leadership; Manuscripts; Mass Spectrum Analysis; Metabolic; Modification; Peptide Synthesis; Peptides; Post-Translational Protein Processing; Preparation; Proteins; Proteomics; Reproducibility; Research; Research Personnel; Resolution; Resource Sharing; Sampling; Scientist; Services; Technical Expertise; Techniques; Technology; Training; Training Support; Training and Education; anticancer research; base; data acquisition; design; equipment acquisition; experience; experimental study; frontier; improved; instrumentation; lipidomics; mass spectrometer; mass spectrometry shared resource; meetings; member; metabolomics; new technology; recruit

Mass Spectrometry Core: Proteomics and Metabolomics Shared Resource - Project Summary/Abstract Since the last renewal, the Mass Spectrometry Core: Proteomics and Metabolomics has been significantly upgraded, including a new Director and staff, the purchase of state-of-the art instrumentation, expansion of Core proteomics services, and the introduction of metabolomics services. By improving every aspect of service, the Mass Spectrometry Core provides Cancer Center members with the highest level of support for their proteomic, metabolomic, and custom peptide synthesis needs. The Director and Associate Director provide expert consultation concerning the design, implementation, and analysis of a wide range of experiments including: protein identification, protein quantification, identification of post-translational modifications, secreted protein/peptide analysis, untargeted lipidomics profiling, targeted lipidomics and metabolomics, bile acids analysis, amino acid quantitation, fatty acid analysis, and custom peptide synthesis. In recent years, five new mass spectrometers have been acquired and a highly experienced Core staff, with strong working knowledge of a wide range of proteomics and metabolomics applications, has been recruited to oversee this facility. The staff provides the knowledge and technical skills to get the most out of this sophisticated new equipment. The Core staff are constantly working to implement new analytical techniques in these rapidly evolving fields while supporting researchers with data analysis, as well as manuscript and grant preparation. Peptide synthesis services have recently been consolidated into this Core and offer custom services for synthesis, HPLC purification, and mass spectrometry-based analysis of peptides, including long peptide syntheses and custom modifications. This Core is broadly used by the vast majority of Cancer Center members, and the staff remain dedicated to meeting and advancing their research. The Core strives to facilitate cancer research by: 1) providing sophisticated instrumentation for discovery: the Core has five high- resolution mass spectrometers (three for proteomics and two for metabolomics) and one peptide synthesizer, 2) providing expertise: decades of combined experience from the Core staff are made readily accessible to Salk investigators for consultations concerning experimental design, data acquisition and analysis, and manuscript and grant preparation, 3) providing critical services: The Core staff provides reproducible, high- quality sample preparation services prior to metabolic and proteomic analysis, as well as providing custom peptide synthesis and purification, 4) providing education, training, and support: Salk researchers at all levels of expertise can receive training in the successful applications of mass spectrometry for proteomic and metabolomics analysis, particularly with respect to experimental design and data analysis, and 5) identifying and evaluating new technologies: the Core seeks to ensure that it continuously offers state-of-the-art instrumentation and validated technological advances to enhance discovery as a result of the Core users' experiments.

质谱核心：蛋白质组学和代谢组学共享资源 - 项目摘要/摘要 自上次更新以来，质谱核心：蛋白质组学和代谢组学已显着 升级，包括任命新的主任和员工、购买最先进的仪器、扩大 核心蛋白质组学服务，并推出代谢组学服务。通过改善各个方面 服务，质谱核心为癌症中心成员提供最高水平的支持 他们的蛋白质组学、代谢组学和定制肽合成需求。董事及副董事 提供有关各种设计、实施和分析的专家咨询 实验包括：蛋白质鉴定、蛋白质定量、翻译后鉴定 修饰、分泌蛋白/肽分析、非靶向脂质组学分析、靶向脂质组学和 代谢组学、胆汁酸分析、氨基酸定量、脂肪酸分析和定制肽合成。 近年来，我们购买了五台新的质谱仪和经验丰富的核心员工， 在广泛的蛋白质组学和代谢组学应用方面拥有丰富的工作知识，已被招募来 监督该设施。员工提供知识和技术技能，以充分利用这一点 先进的新设备。核心人员不断致力于实施新的分析技术 这些快速发展的领域，同时支持研究人员进行数据分析以及手稿和资助 准备。肽合成服务最近已整合到该核心中，并提供定制服务 肽的合成、HPLC 纯化和基于质谱分析的服务，包括长肽 肽合成和定制修饰。该核心被绝大多数癌症中心广泛使用 成员和工作人员仍然致力于会议和推进他们的研究。核心致力于 通过以下方式促进癌症研究：1）提供先进的发现仪器：核心有五个高 分辨率质谱仪（三个用于蛋白质组学，两个用于代谢组学）和一台肽合成仪， 2) 提供专业知识：核心员工数十年的综合经验可以轻松获得 索尔克研究人员就实验设计、数据采集和分析进行咨询，以及 手稿和资助准备，3) 提供关键服务：核心人员提供可重复的、高 在代谢和蛋白质组分析之前提供优质样品制备服务，并提供定制服务 肽合成和纯化，4）提供教育、培训和支持：索尔克各级研究人员 的专业知识可以接受关于质谱法在蛋白质组学和蛋白质组学领域的成功应用的培训 代谢组学分析，特别是实验设计和数据分析，以及 5) 识别 并评估新技术：核心力求确保不断提供最先进的技术 仪器和经过验证的技术进步，以增强核心用户的发现 实验。