Single Cell Spatial Characterization of the Human Bone Marrow Microenvironment

项目摘要/摘要 这个项目的目标是利用高维的单元格方法来描绘空间 人类骨髓的结构。已知造血干细胞的功能受 骨髓中的基质细胞产生的信号。造血细胞之间的地理关系 而特定的基质细胞群被认为是这种相互作用的关键组成部分。在中国学习的同时 动物模型已经阐明了其中许多关键的关系，在人类骨髓中的类似研究也有 是有限的。因此，我们建议使用成像质量细胞术(IMC)来直接询问细胞 人类骨髓微环境的架构。我们将把这种方法与单细胞RNA结合起来 测序，以便能够识别特定基质细胞群体的新标记。这些发现将 将被用来进一步告知国际货币基金组织的做法。在这一背景下，我们将制定常态路线图 人骨髓，可作为正常和疾病后续研究的框架 造血状态。

Project Summary/Abstract The objective of this project is to utilize high dimensional single cell approaches to delineate the spatial architecture of human bone marrow. Functions of hematopoietic stems cells are known to be modulated by signals generated by stromal cells in the bone marrow. Geographic relationships between hematopoietic cells and specific stromal cell populations are thought to be a critical component of this interaction. While studies in animal models have elucidated many of these key relationships, similar studies in human bone marrow have been limited. We therefore propose to utilize imaging mass cytometry (IMC) to directly interrogate the cellular architecture of the human bone marrow microenvironment. We will couple this approach with single cell RNA sequencing to enable the identification of novel markers of specific stromal cell populations. These findings will be leveraged to further inform the IMC approach. Within this context, we will develop a road map of normal human bone marrow, which can serve as the framework for subsequent studies of both normal and diseased hematopoietic states.