Random Field Modelling of Genetic and Epigenetic Association for Congenital Heart Defects in the Presence of Disease Heterogeneity

项目摘要/摘要 这项关于指导研究科学家发展奖的提议旨在为候选人提供 过渡到独立的统计遗传学家和遗传流行病学家所需的时间和资源 心血管疾病，特别是先天性心脏病。这一目标将通过 加强培训和研究经验：1)参加与出生缺陷有关领域的教学课程 流行病学、生殖遗传学和综合基因组学方法；2)实施研究 CHD基因组和表观基因组研究项目；以及3)召集一个由导师组成的专家小组 广泛而互补的技能。拟议的K01计划将补充候选人之前的 接受统计学、流行病学和数量生物学方面的培训，优化他完成拟议研究的努力 项目，并允许他成为一名独立的调查员。(共同)导师刘念军博士和约翰·S。 Witte将为候选人在综合基因组学方法学领域的培训提供建议。(共同)导师， 夏洛特·A·霍布斯博士、本杰明·季奇科博士和加利·韩博士将为候选人在出生领域的培训提供建议 遗传学、流行病学和生殖遗传学。在拟议的项目中，创新的生物统计方法 将被开发并应用于国家出生缺陷预防研究(NBDPS)的样本，这是最大的 对出生缺陷进行的多点人群研究。我们将利用基因组数据(即~500万 1,000例母亲-父亲-婴儿三联体和约1,000名对照母婴二联体的遗传变异)，以及 71例心脏组织标本的表观遗传学数据(即~450K甲基化位点)。拟议的项目将解决 CHD研究中的三个挑战：1)CHD的遗传异质性，也就是众所周知的“所有幸福的家庭都是一样的， 每个不幸的家庭都有自己的不幸“；2)特定组织内遗传变异的功能影响， 如遗传变异和表观遗传修饰之间的联系；以及3)复杂的相互作用 潜在的CHD，包括遗传变异之间的高阶相互作用，表观遗传修饰和 母性生活方式因素。候选人成功地完成了为期两年的KL2指导职业生涯 发展奖，题为“检测基因与环境之间的相互作用” 提供了一个基础，我们将在此基础上进行更详细的研究，以解决拟议的 主题。这些发现可能会为深入了解潜在的病理生理和病因过程提供帮助。 这导致了CHD，更重要的是，可以为翻译研究提供方向，从而导致更多 精确的孕前咨询和干预。通过培训和研究获得的经验 拟议的研究将作为独立研究计划的基础，包括新的R01建议 以扩展将通过这项拟议的K01计划启动的研究。

Project Summary/Abstract This proposal for a Mentored Research Scientist Development Award is designed to provide the candidate the necessary time and resources to transit into an independent statistical geneticist and genetic epidemiologist in cardiovascular diseases, particularly in congenital heart defects (CHDs). This goal will be achieved through enhanced training and research experience: 1) by taking didactic courses in areas related to birth defects epidemiology, reproductive genetics and integrative genomics methodologies; 2) by implementing research projects for genomic and epigenomic studies of CHDs; and 3) by assembling an expert panel of mentors with extensive and complementary skills. The proposed K01 program will complement the candidate's previous training in statistics, epidemiology and quantitative biology, optimize his effort to complete the proposed research projects, and allow him to become an independent investigator. The (co-)mentors, Drs. Nianjun Liu and John S. Witte, will advise the candidate's training in the domain of integrative genomics methodologies. The (co-)mentors, Drs. Charlotte A. Hobbs, Benjamin Tycko and Jiali Han will advise the candidate's training in the domain of birth genetics epidemiology and reproductive genetics. In the proposed projects, innovative biostatistical approaches will be developed and applied to samples from the National Birth Defect Prevention Study (NBDPS), the largest multi-site population-based study of birth defects ever conducted. We will utilize the genomic data (i.e. ~ 5 million genetic variants) of ~ 1,000 case mother-father-infant triads and ~ 1,000 control mother-infant dyads, and the epigenetic data (i.e. ~ 450K methylation sites) of 71 cardiac tissue samples. The proposed projects will address three challenges in CHD research: 1) the genetic heterogeneity of CHDs, known as “all happy families are alike, each unhappy family is unhappy in its own way”; 2) the functional effects of genetic variants within specific tissues, such as the association between genetic variations and epigenetic modifications; and 3) the complex interactions underlying CHDs, including the high-order interactions among genetic variants, epigenetic modifications and maternal life style factors. The candidate has successfully completed a two-year KL2 Mentored Career Development Award, entitled “detecting gene-by-gene and gene-by-environment interactions associated with CHDs”, which provides a foundation onto which we will build more detailed studies to address the proposed topics. The findings will likely provide insights into the underlying pathophysiological and etiological processes that result in CHDs, and more importantly, can provide a direction for translational research leading to more precise preconceptional counseling and interventions. The training and research experience gained from the proposed study will serve as the groundwork for an independent research program, including a new R01 proposal to expand upon the research that will be initiated through this proposed K01 program.