The Gastrointestinal Experimental Model Systems (GEMS) Core

胃肠实验模型系统 (GEMS) 核心

• 批准号: 10117232
• 负责人: NOAH Freeman SHROYER
• 金额: $ 17.6万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-01 至 2023-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10117232
• 关键词: 3-Dimensional; Advisory Committees; Animals; Barrett Esophagus; Biological Models; Biology; Cancer Patient; Cell Culture Techniques; Cells; Clinical; Collection; Colonic Adenoma; Colorectal Cancer; Consultations; Culture Media; Derivation procedure; Development; Differentiation and Growth; Digestive System Disorders; Disease; Education and Outreach; Experimental Models; Generations; Germ-Free; Gnotobiotic; Goals; Health; Hospitals; Human; Immigrant; Immunodeficient Mouse; Inflammatory Bowel Diseases; Institutional Review Boards; Instruction; Insurance; Intestines; Maintenance; Medical center; Medicine; Modeling; Mus; Organoids; Patients; Pediatrics; Physiology; Privatization; Production; Protocols documentation; Published Comment; Reagent; Recommendation; Research Personnel; Rodent; Sampling; Services; Specific qualifier value; Specimen; Surveys; Technology; Texas; Tissue Sample; Training; Uninsured; Veterans; Xenograft procedure; animal facility; animal tissue; base; biobank; college; cost; embryonic stem cell; gastrointestinal; human pluripotent stem cell; human tissue; improved; induced pluripotent stem cell; matrigel; member; monolayer; neoplastic; patient population; pre-clinical; success; two-dimensional

Project Summary The Gastrointestinal Experimental Model Systems (GEMS) core is a newly reorganized of the Texas Medical Center-Digestive & Disease Center (TMC-DDC) in 2015. Based on examination of prior usage, reviewer comments from the previous competitive renewal, and the recommendation of the external advisory committee, the GEMS core is organized to encompass 1) an enteroid/organoid subcore and 2) a gnotobiotic subcore. The DDC successfully obtained Baylor College of Medicine (BCM) support to establish a gnotobiotic animal facility during the previous Project Period. This reorganization was based on the exciting scientific developments in the field, our unique expertise, increased DDC investigator need and use of enteroids and gnotobiotic animals in conjunction with decreased use of the physiology services offered by the previous Integrative Biology Core. To accomplish these aims, we will provide services, including the following: • Human enteroids from the TMC-DDC biobank: as 3-dimensional enteroids, monolayers or transwells • Murine enteroids from the TMC-DDC biobank • Complete growth media or components for growth and differentiation of human and animal enteroids • Derivation of new primary enteroids from animals or human tissue specimens • Derivation of stably transduced enteroid lines • Human pluripotent stem cell-derived intestinal organoids, made from embryonic stem cells or iPS cells • Xenograft generation of enteroids/organoids into immunodeficient mice • Production and maintenance of germ-free rodents • Technical services for studies involving gnotobiotic rodents • Creation of germ-free mice de novo • Training, outreach, and consultative services Importantly, there is no other similar facility in the TMC or nearby in the region. This Core is highly synergetic with the other scientific cores of the TMC-DDC. For example, extensive cooperation with Core E (Clinical Core) facilitates the development and approval of new IRB protocols and collection/tracking of tissue samples required to establish new human enteroid lines. Evidence of the success of this interaction is apparent in the >120 enteroids lines that are already established and the continued accrual of new samples targeting specific patient populations.

项目摘要 胃肠道实验模型系统(GEMS)核心是德克萨斯州最新重组的 医疗中心-消化与疾病中心(TMC-DDC)于2015年成立。基于对先前使用的检查， 审查员对前一次竞争续签的评论，以及外部 咨询委员会，GEMS核心的组织包括1)肠样/器官亚核和2)A 灵知生物亚核。DDC成功地获得贝勒医学院(BCM)的支持，建立了 在前一项目期间的灵知生物动物设施。这一重组是基于 该领域令人振奋的科学发展，我们独特的专业知识，增加了DDC调查员的需求和使用 减少使用所提供的生理学服务的肠类动物和灵生动物 由之前的综合生物学核心。为达致这些目标，我们会提供服务，包括 以下内容： ·来自TMC-DDC生物库的人肠样：作为三维肠样、单层或跨孔 ·来自TMC-DDC生物库的小鼠肠样 ·用于人和动物肠道生长和分化的完整生长介质或成分 ·从动物或人类组织标本中衍生出新的初级肠样 ·稳定传导的肠状线的派生 ·人类多能干细胞来源的肠道器官，由胚胎干细胞或iPS制成 单元格 ·免疫缺陷小鼠异种移植类肠/类器官 ·生产和维护无菌啮齿动物 ·为涉及群生啮齿动物的研究提供技术服务 ·创造无菌小鼠从头开始 ·培训、外联和咨询服务 重要的是，在TMC或附近地区没有其他类似的设施。这一核心是高度 与TMC-DDC的其他科学核心协同作用。例如，与Core的广泛合作 E(临床核心)促进新IRB方案的开发和批准以及收集/跟踪 建立新的人类肠样线所需的组织样本。这种互动成功的证据 这在已经建立的&gt；120肠状肌系列中很明显，而且新的 针对特定患者群体的样本。