The Gastrointestinal Experimental Model Systems (GEMS) Core

项目摘要 胃肠道实验模型系统（GEMS）的核心是一个新重组的得克萨斯州的 2015年，医学中心-消化与疾病中心（TMC-DDC）。基于对先前使用的检查， 上一次竞争性续约的评审意见，以及外部 根据咨询委员会的建议，GEMS核心被组织为包括1）肠状/类器官亚核心和2） 知生亚核DDC成功获得贝勒医学院（Baylor College of Medicine）的支持， 在上一个项目期间，一个生物动物设施。这次重组是基于 该领域令人兴奋的科学发展，我们独特的专业知识，增加DDC研究人员的需求和使用 肠类动物和知菌动物的死亡，以及所提供的生理服务的使用减少 综合生物学的核心。为达致这些目标，我们会提供服务，包括 如下： ·来自TMC-DDC生物库的人类肠：作为3维类肠、单层或transwell ·来自TMC-DDC生物库的鼠类肠 ·用于人类和动物类肠细胞生长和分化的完整生长培养基或组分 ·从动物或人类组织样本衍生新的原代类肠 ·稳定转导的类肠细胞系的衍生 ·人类多能干细胞衍生的肠类器官，由胚胎干细胞或iPS制成 细胞 ·将类肠/类器官异种移植到免疫缺陷小鼠中 ·无菌啮齿动物的生产和维护 ·为涉及致菌啮齿动物的研究提供技术服务 ·从头创建无菌小鼠 ·培训、推广和咨询服务 重要的是，TMC或该地区附近没有其他类似的设施。核心是高度 与TMC-DDC的其他科学核心协同。例如，与Core的广泛合作 E（临床核心）促进了新IRB方案的制定和批准，并收集/跟踪 建立新的人类肠系所需的组织样本。这种互动成功的证据 在已经建立的>120条肠线和新的肠线的持续增加中是明显的。 针对特定患者群体的样本。

Project Summary The Gastrointestinal Experimental Model Systems (GEMS) core is a newly reorganized of the Texas Medical Center-Digestive & Disease Center (TMC-DDC) in 2015. Based on examination of prior usage, reviewer comments from the previous competitive renewal, and the recommendation of the external advisory committee, the GEMS core is organized to encompass 1) an enteroid/organoid subcore and 2) a gnotobiotic subcore. The DDC successfully obtained Baylor College of Medicine (BCM) support to establish a gnotobiotic animal facility during the previous Project Period. This reorganization was based on the exciting scientific developments in the field, our unique expertise, increased DDC investigator need and use of enteroids and gnotobiotic animals in conjunction with decreased use of the physiology services offered by the previous Integrative Biology Core. To accomplish these aims, we will provide services, including the following: • Human enteroids from the TMC-DDC biobank: as 3-dimensional enteroids, monolayers or transwells • Murine enteroids from the TMC-DDC biobank • Complete growth media or components for growth and differentiation of human and animal enteroids • Derivation of new primary enteroids from animals or human tissue specimens • Derivation of stably transduced enteroid lines • Human pluripotent stem cell-derived intestinal organoids, made from embryonic stem cells or iPS cells • Xenograft generation of enteroids/organoids into immunodeficient mice • Production and maintenance of germ-free rodents • Technical services for studies involving gnotobiotic rodents • Creation of germ-free mice de novo • Training, outreach, and consultative services Importantly, there is no other similar facility in the TMC or nearby in the region. This Core is highly synergetic with the other scientific cores of the TMC-DDC. For example, extensive cooperation with Core E (Clinical Core) facilitates the development and approval of new IRB protocols and collection/tracking of tissue samples required to establish new human enteroid lines. Evidence of the success of this interaction is apparent in the >120 enteroids lines that are already established and the continued accrual of new samples targeting specific patient populations.