Coordinating hunger and thirst drives in Drosophila

喂养决定是根据内部需求严格监管的。饥饿和口渴的信号是 由促进特定消费以恢复动态平衡的内感神经元检测到。虽然 通常被认为是独立的，最近的研究表明，饥饿和 Thirst Drive对于协调相互竞争的需求非常重要。这项提议的目的是研究食物和食物如何 以发达的果蝇系统为模型，协调调节耗水量。这些 研究将评估多个内部信号如何在神经元内整合以调节活动，以及如何 一组神经元的活动调节着两个对手的行为。这项研究利用了 建立了果蝇消耗水和蔗糖的行为分析，分子遗传学迅速 操纵基因和神经功能，单细胞电生理学，以精确测量信号 整合，以及研究网络相互作用的大规模钙成像方法。具体目标1将 研究饥饿和口渴的信号如何调节感觉间神经元的活动，从而洞察如何 随着时间的推移，多个信号集成在一个神经元中。《特定目标2》将描述下游神经元的特征 来研究活动如何传递到电路，从而相反地调节两个神经元 行为。具体目标3将确定感觉间神经元如何改变味觉运动的反应。 研究内部状态如何在馈电网络中产生塑料变化的电路。拟议的努力将 确定营养状态的内部信号如何随时间整合，以协调喂养决策。 这项工作的长期目标是深入了解神经调节剂如何调节电路和 在长时间尺度上的行为，这是神经科学中跨系统相关的一个基本问题。

Feeding decisions are tightly regulated based on internal needs. Signals of hunger and thirst are detected by interoceptive neurons that promote specific consumption to restore homeostasis. Although generally considered independently, recent studies have demonstrated that interactions between hunger and thirst drives are important to coordinate competing needs. The aim of this proposal is to examine how food and water consumption is coordinately regulated, using the well developed Drosophila system as a model. These studies will evaluate how multiple internal signals are integrated within a neuron to regulate activity and how the activity of a single set of neurons regulates two opponent behaviors. This research takes advantage of established behavioral assays for water and sucrose consumption in Drosophila, molecular genetics to rapidly manipulate genes and neural function, single-cell electrophysiology for precise measurements of signal integration, and large-scale calcium imaging approaches to study network interactions. Specific Aim 1 will examine how signals of hunger and thirst modulate activity of interoceptive neurons, providing insight into how multiple signals are integrated within a neuron over time. Specific Aim 2 will characterize neurons downstream of the interoceptive neurons to examine how activity is transmitted to circuits to oppositely regulate two behaviors. Specific Aim 3 will determine how interoceptive neurons alter the responses in taste sensorimotor circuits to examine how internal states generate plastic changes in feeding networks. The proposed efforts will determine how internal signals of nutritional state are integrated across time to coordinate feeding decisions. The long-term objective of this work is to provide insight into how neuromodulators regulate circuits and behavior over long timescales, a basic problem in neuroscience relevant across systems.