The effect of Inhaled Nicotine on Pulmonary Surfaces

吸入尼古丁对肺表面的影响

• 批准号: 10083514
• 负责人: Mehmet Kesimer
• 金额: $ 6.29万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-06 至 2021-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10083514
• 关键词: Acute; Affect; Antiinflammatory Effect; Antimicrobial Effect; Biological; Blood; Bronchiectasis; Cardiovascular Diseases; Cells; Chronic; Chronic Obstructive Airway Disease; Data; Disease; Drug Kinetics; Electronic cigarette; Epithelial; Epithelium; Escalator; General Population; Glycerol; Grant; Health; Human; Hydration status; Impairment; In Vitro; Inhalation; International; Journals; Knowledge; Leukocyte Elastase; Lung; Malignant neoplasm of lung; Mass Spectrum Analysis; Measures; Metabolic; Metabolism; Metalloproteases; Mucins; Mus; Nicotine; Nicotinic Receptors; Parents; Patients; Peer Review; Peptide Hydrolases; Pharmacodynamics; Phase; Play; Process; Property; Propylene Glycols; Proteins; Proteomics; Publications; Publishing; Pulmonary Emphysema; Research; Research Personnel; Respiratory System; Role; Sampling; Scientist; Smoking; Sputum; Structure; Surface; System; Testing; Tobacco; Toxic effect; Toxin; Urine; Vegetables; Western Blotting; Writing; absorption; addiction; airway epithelium; antimicrobial; base; biophysical techniques; bronchial epithelium; cell type; defense response; design; electronic cigarette use; experimental study; human subject; in vitro testing; interest; meetings; mortality; neutrophil; nicotine inhalation; nicotine use; parent grant; pathogen; pulmonary function; vaper; vaping

Abstract In the parent grant, we have proposed to study the effect of nicotine on pulmonary surfaces. That is, we have proposed to look at the effects of nicotine on key components of innate defense relevant to the lung using a variety of cell biological and biophysical techniques. We have subsequently requested a revision to the grant to include human subjects so that we can also study the effects of nicotine on innate defense cells derived from humans including neutrophils. In the interim however, we have identified a major knowledge gap, namely that we do not fully appreciate the levels of nicotine that occur in vapers’ lungs during and after vaping. This is an impediment to our research since we do not know which concentrations of nicotine to add to our in vitro experiments. Thus, in this supplement, Dr. Abdelwahab will measure nicotine levels in vaped humans and mice and will also perform pharmacokinetic studies of nicotine on pulmonary surfaces in vitro. These studies will be important not only for our research but for other researchers in the field since the knowledge does not exist. Deciding how to perform the pharmacokinetics will be an iterative process and Dr. Abdelwahab will have intellectual input into how and when to collect nicotine. He will also decide how to measure nicotine using mass spectrometry-type approaches and will design the experiments to determine how long nicotine stays around on airway surfaces. These studies will enable Dr. Abdelwahab to develop independent as a scientist since they will allow him to design and execute important experiments that will be of international interest. Moreover, should these experiments be successful, it is also fully expected that he will have the opportunity to present these data at national and international meetings and to write them up for publication in peer-reviewed journals.

抽象的 在父母的赠款中，我们提议研究尼古丁对肺部表面的影响。也就是说，我们有 提议使用A 各种细胞生物学和生物物理技术。随后，我们要求对赠款进行修订 包括人类受试者，以便我们还可以研究尼古丁对来自先天防御细胞的影响 人类包括中性粒细胞。但是，在临时，我们已经确定了一个主要的知识差距，即 我们不完全欣赏vapers肺中和蒸气后肺中发生的尼古丁的水平。这是一个 障碍我们的研究障碍，因为我们不知道要添加哪些浓度的尼古丁浓度 实验。在这种补充中，Abdelwahab博士将测量蒸汽人和小鼠中的尼古丁水平 并将在体外对尼古丁进行药代动力学研究。这些研究将是 不仅对我们的研究重要，而且对于该领域的其他研究人员来说重要，因为知识不存在。 决定如何执行药代动力学将是一个迭代过程，Abdelwahab博士将 智力输入如何以及何时收集尼古丁。他还将决定如何使用质量测量尼古丁 光谱法型接近，并将设计实验以确定尼古丁在多长时间内待在 气道表面。这些研究将使Abdelwahab博士成为科学家的独立发展，因为他们将 允许他设计并执行将具有国际关注的重要实验。而且，应该 这些实验取得了成功，也完全期望他将有机会呈现这些数据 在国家和国际会议上，并将其写成在同行评审期刊上出版。