Developing powerful daisy drive systems for the precise alteration of local populations

项目摘要和相关性 基因驱动系统可以通过改变整个野生种群来解决生态问题 有机体然而，目前基于CRISPR的驱动系统的自我维持性质使它们 很可能会传播到大多数当地种群，也很可能传播到该物种的其他种群 在世界其他地方。如果没有一种安全测试手段或确保国际协议， 部署，目前还不清楚如何在道德上释放这些类型的基因驱动系统。这项建议 旨在开发能够精确和经济地改变当地人口的“菊花驱动”系统， 野生生物。具体来说，该项目将开发降低两者健身成本的方法。 改变和抑制驱动系统，精确控制它们将传播的程度， 并完美逆转其他类型的基于CRISPR的驱动系统传播的遗传变化。 通过提供一条途径， 部署，这一建议将有助于预防许多病媒传播和寄生虫病， 通过在病媒中传播抗病性或直接抑制疾病种群而传播疾病 剂.我们预计雏菊驱动干预措施将成为适合的强大武器 抗击疟疾、血吸虫病、登革热、寨卡病毒、莱姆病等等。

Project Summary and Relevance Gene drive systems could solve ecological problems by altering entire populations of wild organisms. However, the self-sustaining nature of current CRISPR-based drive systems makes them likely to spread to most local populations, and very possibly to other populations of that species elsewhere in the world. Without a means of safe testing or securing international agreement prior to deployment, it is unclear how these types of gene drive systems can be ethically released. This proposal seeks to develop “daisy drive” systems capable of precisely and economically altering local populations of wild organisms. Specifically, the project will develop ways of reducing the fitness cost of both alteration and suppression drive systems, precisely controlling the extent to which they will spread, and perfectly reversing genetic changes spread by other types of CRISPR-based drive systems. By providing a path to community-supported, diplomatically feasible, and economical deployment, this proposal will facilitate the prevention of numerous vector-borne and parasitic diseases by spreading disease resistance among vectors or directly suppressing populations of disease agents. We anticipate that daisy drive interventions will serve as powerful weapons suitable for combating malaria, schistosomiasis, dengue, Zika, Lyme disease, and many more.