Novel vacuole biology in chronic Toxoplasma infection

摘要 口服弓形虫包囊可建立一种慢性感染，其特征是发生 中枢神经系统的囊肿症。中枢神经系统中弓形虫休眠包囊的复活 艾滋病患者的系统会导致弓形体脑炎，这是一种临床上难以治疗并经常致命的疾病 脑炎。导致包囊的慢性持久性和口腔传染性的寄生虫因素仍然是 人们对此知之甚少。我们推测ROP35蛋白和弓形虫的其他成员 WNG激酶家族在维持持续性包囊及其口腔感染性方面起着重要作用。本研究 将表征ROP35在介导成功的慢性感染中的作用，以及WNG激酶的作用， 以及它们的蛋白质复合体，使活的和口腔感染的弓形虫持续存在 囊肿症。

Abstract Oral ingestion of Toxoplasma gondii cysts establishes a chronic infection marked by the development of cysts in the central nervous system. Reactivation of dormant Toxoplasma gondii cysts in the central nervous system in AIDS patients causes Toxoplasmic encephalitis, a clinically difficult to treat and frequently lethal encephalitis. The parasite factors that enable the chronic persistence and oral infectivity of cysts are still poorly understood. We hypothesize that the ROP35 kinase and other members of the Toxoplasma gondii WNG kinase family play important roles in maintaining persisting cysts and their oral infectivity. This study will characterize the role of ROP35 in mediating successful chronic infection, and the role of WNG kinases, and their protein complexes, that enable the persistence of viable and orally infectious Toxoplasma gondii cysts.