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Novel vacuole biology in chronic Toxoplasma infection

慢性弓形虫感染中的新型液泡生物学

基本信息

批准号：
10092083
负责人：
DAVID J BZIK
金额：
$ 24.6万
依托单位：
DARTMOUTH COLLEGE
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-02-04 至 2023-01-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10092083
关键词：
Acquired Immunodeficiency Syndrome Acute Antigen Presentation Antigens Appearance Biological Assay Biology CD8-Positive T-Lymphocytes Cells Cessation of life Chronic Clinical Clinical Management Complex Cyst Data Development Encephalitis Family Gene Deletion Genetic Granzyme Growth Histocompatibility Hospitalization Immune In Vitro Infection Interferons Maintenance Measures Mediating Medical Membrane Microglia Morphology Mutation Natural Immunity Neuraxis Oocysts Oral Oral Ingestion Parasites Patients Penetration Phase Phenotype Phosphotransferases Play Population Proteins Reporting Resistance Role Structure T cell response T-Lymphocyte Testing Toxoplasma Toxoplasma gondii United States Vacuole Virulence base chronic infection foodborne pathogen in vivo insight macrophage member mutant novel oral infection perforin pressure prevent protein complex rhoptry toxoplasmic encephalitis

项目摘要

Abstract Oral ingestion of Toxoplasma gondii cysts establishes a chronic infection marked by the development of cysts in the central nervous system. Reactivation of dormant Toxoplasma gondii cysts in the central nervous system in AIDS patients causes Toxoplasmic encephalitis, a clinically difficult to treat and frequently lethal encephalitis. The parasite factors that enable the chronic persistence and oral infectivity of cysts are still poorly understood. We hypothesize that the ROP35 kinase and other members of the Toxoplasma gondii WNG kinase family play important roles in maintaining persisting cysts and their oral infectivity. This study will characterize the role of ROP35 in mediating successful chronic infection, and the role of WNG kinases, and their protein complexes, that enable the persistence of viable and orally infectious Toxoplasma gondii cysts.

摘要口服弓形虫包囊可导致慢性感染，其特征为：中枢神经系统的囊肿中枢神经系统内休眠弓形虫包囊的再激活艾滋病患者体内的弓形虫系统引起弓形虫脑炎，这是一种临床上难以治疗且经常致命的疾病，脑炎使囊肿具有慢性持久性和口腔传染性的寄生虫因素仍然存在。不太了解。我们假设ROP 35激酶和弓形虫的其他成员 WNG激酶家族在维持囊肿的持续存在及其口腔感染性中起重要作用。本研究将表征ROP 35在介导成功慢性感染中的作用，以及WNG激酶的作用，以及它们的蛋白复合物，使存活的和经口感染的弓形虫能够持续存在囊肿