Leica LMD7000 Laser Microdissection System

点击翻译按钮获取中文摘要

DESCRIPTION (provided by applicant): The Histology and Microscopy Core facility at the Ernest Gallo Clinic and Research Center (EGCRC) at the University of California, San Francisco provides shared equipment and training in methods that use microscopy for investigating molecular and structural changes in the brain that underlie drug and alcohol dependence. These questions have been traditionally addressed using immunocytochemistry and in situ hybridization, and for the past decade, with the NIH-developed method of laser capture microdissection (LCM), which offers the possibility of selectively collecting specific cell populations from counterstained histological sections. The original infrared (IR) laser-based PixCell IIe system (Arcturus) was purchased by the EGCRC in 2001. Since then this instrument has allowed our investigators to measure mRNA abundance in subpopulations of invertebrate and mammalian cells by RT-PCR and microarray technology. However, over the last several years, technical drawbacks with this IR laser-based LCM design have become evident. First, it can optimally use only 5-8 micron thick sections and is thus limited to analysis of small amounts of tissue. Second, our PixCell IIe system is manual and cannot outline and then dissect out a brain region using an automated function. Furthermore, to obtain the most effective IR pulses, one must refocus the manual laser before and during tissue collection, which is very time-consuming. In addition, investigating regions of the brain infected with viruses for RNA interference studies has proven very difficult to perform due to the need to collect many thousands of cells from each animal. Therefore, despite a significant need for LCM in our experiments, our current system is extremely inadequate. Recently, LCM vendors have developed systems that use ultraviolet (UV) lasers as a cutting tool and this development has proven to be a major improvement. Since our PixCell IIe is not upgradeable, we now seek to acquire the most up-to-date instrument, an LDM7000 from Leica Microsystems. The requested instrument will be equipped with the highly effective UV cutting laser, automated region collection and other options that will greatly assist in obtaining better samples at reduced cost and time. With the LDM7000 our investigators will be able to continue to perform NIH-funded experiments and avoid the serious limitations imposed by the outdated equipment currently at the EGCRC.