IVIS Spectrum Imaging System

描述（由申请人提供）：在研究环境中，先进的光学成像设备有可能扩大非侵入性成像在科学发现中的作用。我们建议购买最先进的生物发光/荧光成像系统（IVIS光谱）。在比较了可用的系统后，我们决定来自Caliper Life Sciences（Boston MA）的IVIS Spectrum最能满足我们研究社区的需求。这样的系统不仅将大大提高我们的体内光学成像能力，而且还允许与磁共振成像（MRI）和高分辨率微型CT扫描收集的数据集成。UCSD拥有世界上最先进的功能性MRI成像设施之一，这些互补的成像方式将使研究人员能够在以前不可能实现的水平上进行研究。定位于三维定量解剖结构的信号将提高从正常发育到疾病病理的研究的精度。此外，有许多类型的实验可以被设计成离体成像组织和细胞，并利用该成像系统进行高通量筛选。重要的是，该光学成像设施将成为正在开发的多模块生理学核心的一部分，以提供小动物功能测定，包括心血管动力学，代谢分析和肺功能。来自这些多个小动物功能模块的支持将补充成像模式，以更好地理解所收集的成像数据的生理基础。医学院医学教学设施社区（尽管名称，这主要是一个面向研究的社区）是在一个全面的资源，将非侵入性成像数据与方法，以评估综合生理功能的变化的迫切需要。所有MTF研究人员都可以使用这种最先进的光学成像数据采集方法，软件以及数据处理，图像分析和数据集成方法的培训和教育。随着采集和分析技术的发展加速，研究人员将光学成像纳入其研究计划的核心将为构成UCSD社区的研究人员提供知识基础，将使这些新工具和成像技术广泛使用，并鼓励更广泛地使用这些技术。加州大学圣地亚哥分校和大圣地亚哥社区有强大的研究计划，这个成像中心将增加研究人员实现甚至超过他们的研究目标的能力。 公共卫生相关性：光学成像是在动物模型中非侵入性研究生物过程的重要工具。了解和监测健康和疾病状态下生理系统的动态变化将大大提高科学界诊断和防治各种疾病的能力。

DESCRIPTION (provided by applicant): In research settings advanced optical imaging facilities have the potential to expand the role of non- invasive imaging for scientific discovery. We are proposing to purchase a state of the art bioluminescent/fluorescent imaging system (the IVIS Spectrum). After comparing available systems we have decided that the IVIS Spectrum from Caliper Life Sciences (Boston MA), best serves the needs of our research community. Such a system will not only greatly increase our in vivo optical imaging capabilities, but also allow integration with data collected by magnetic resonance imaging (MRI) and high resolution micro CT scanning. UCSD has one of the finest state of the art functional- MRI imaging facilities the world, and together these complementary imaging modalities will allow investigators to perform research at a level impossible to achieve before. Signals localized to three- dimensional quantitative anatomical structures will enhance precision in studies ranging from normal development to disease pathologies. Moreover, there are many types of experiments that can be designed to image tissues and cells ex vivo, and utilize this imaging system for high throughput screening. Importantly, this optical imaging facility will be part of a multi-module Physiology Core being developed to provide small animal functional assays, which include cardiovascular dynamics, metabolic analysis, and pulmonary function. Support from these multiple small animal function modules will complement imaging modalities to better understand the physiological bases of collected imaging data. The School of Medicine Medical Teaching Facility community (despite the name this is primarily a research oriented community) is in critical need of a comprehensive resource for incorporating non-invasive imaging data with methods to assess changes in integrative physiological function. All MTF researchers will have access to this state-of-the-art optical imaging data acquisition method, software, as well as training and education for data processing, image analysis, and data integration methods. As development of acquisition and analysis techniques accelerate, the core of investigators incorporating optical imaging into their research programs will provide a knowledge base for investigators that make up the UCSD community, will make these new tools and imaging techniques widely available, and encourage broader use of these technologies. UCSD and the greater San Diego community have strong research programs and this imaging center will add to the ability of researchers to achieve and perhaps even exceed their research goals. Public Health Relevance: Optical imaging is an essential tool for non-invasively studying biological processes in animal models. Understanding and monitoring dynamic changes in physiological systems in both health and disease states will greatly improve the scientific communities ability to diagnose and combat a wide range of illnesses.