MoFlo XDP High Speed Cell Sorter

MoFlo XDP 高速细胞分选仪

• 批准号: 7794963
• 负责人: CELSA A SPINA
• 金额: $ 43.99万
• 依托单位: VETERANS MEDICAL RESEARCH FDN/SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-05-20 至 2011-05-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7794963
• 关键词: 15 year old; Acquired Immunodeficiency Syndrome; Affect; Arts; Award; Biology; Blood; Brain; Cardiology; Cell Separation; Cells; Communicable Diseases; Communities; Community Services; Computer software; Core Facility; Development; Discipline; Disease; Electronics; Endocrine system; Endocrinology; Equipment; Evaluation; Flow Cytometry; Foundations; Funding; Future; Gastroenterology; Generations; Genetic; Goals; Grant; Grant Review; Health Sciences; Healthcare Systems; Heart; Hematology; Immune; Individual; Investigation; Laboratories; Maintenance; Malignant Neoplasms; Medical Research; Modeling; National Center for Research Resources; Neurobiology; Postdoctoral Fellow; Research; Research Personnel; Research Support; Resources; Services; Speed; Technology; Time; United States National Institutes of Health; Update; Veterans; Wages; Work; base; cost; design; drug development; improved; infectious disease treatment; instrument; instrumentation; meetings; oncology; public health relevance

DESCRIPTION (provided by applicant): MoFlo XDP High Speed Cell Sorter We are proposing to replace an existing older cell sorter instrument within a core facility with a new upgraded model, a MoFlo XDP High Speed Cell Sorter manufactured by Beckman Coulter. The requested new instrument represents much improved "second generation" technology that is required to meet the needs of current, and future, research goals of the general San Diego academic community. The existing instrument (MoFlo Legacy), which we propose to replace, was obtained in 1997 with a grant award from the NCRR. At the time of acquisition, this equipment represented cutting-edge technology in cell sorting design; however, today the instrumentation reflects operational electronics and software that is approximately 15 years old. The current needs of the investigative group that utilizes our cell sorting facility are rapidly exceeding the capabilities of the existing instrumentation. Individual investigator-based laboratories cannot normally support the initial costs of a cell sorter purchase and the recurring expenses of instrument maintenance and dedicated operator salaries. The most effective and economical use of flow cytometric technology in an academic research setting is through access provided by a centralized core resource. To this end, the San Diego CFAR / VMRF Flow Cytometry Research Core is cooperatively supported by the Veterans Medical Research Foundation (VMRF), the UCSD Center for AIDS Research (CFAR), and the Division of Research of the VA San Diego Healthcare System (VASDHS). The core is highly regarded throughout the local research community and the services provided receive consistently excellent evaluations. The facility is well utilized by over 50 individual investigators and their associated research groups. Currently, 22 of these investigators require cell sorting to support their research efforts. This combined endeavor entails funding by a total of 31 NIH grant awards and at least 6 additional non-NIH awards (such as VA Merit Review grants). The research supported through core services covers a broad spectrum of health science fields that include infectious diseases, neurobiology, hematology, cardiology, oncology, gastroenterology, endocrinology, and genetics. In summary, acquisition of the MoFlo XDP High Speed Cell Sorter will enable the San Diego CFAR / VMRF Flow Cytometry Research Core to continue to provide "state-of-the-art" services to the local academic research community to support investigations that span disciplines from basic biology to translational medical research to drug development. PUBLIC HEALTH RELEVANCE: We are requesting funding to replace an existing older, major piece of equipment (cell sorter) with a new updated model that will be located within a central core laboratory. This equipment will provide services to support the research efforts of 22 different investigators, whose work is critical to the development of new treatments for infectious diseases, cancer and diseases that affect the blood, brain, heart, and immune, digestive, and endocrine systems.

描述（由申请人提供）：MoFlo XDP高速细胞分选仪我们建议用Beckman Coulter制造的新升级型号MoFlo XDP高速细胞分选仪替换核心设施内现有的旧细胞分选仪仪器。所要求的新仪器代表了大大改进的“第二代”技术，需要满足当前和未来的需要，一般圣地亚哥学术界的研究目标。我们建议取代的现有工具（MoFlo Legacy）是1997年从NCRR获得的赠款。在收购时，该设备代表了细胞分选设计中的尖端技术;然而，今天的仪器反映了大约15年前的操作电子设备和软件。使用我们的细胞分选设备的研究小组目前的需求正在迅速超过现有仪器的能力。 单个基于细胞分选机的实验室通常无法支持购买细胞分选机的初始成本以及仪器维护和专门操作员工资的经常性费用。在学术研究环境中最有效和最经济地使用流式细胞仪技术是通过集中的核心资源提供的访问。为此，圣地亚哥CFAR / VMRF流式细胞术研究核心由退伍军人医学研究基金会（VMRF），UCSD艾滋病研究中心（CFAR）和VA圣地亚哥医疗保健系统研究部门（VASDHS）合作支持。核心是高度重视整个当地的研究界和提供的服务获得一贯优秀的评价。该设施得到50多名调查人员及其相关研究小组的充分利用。目前，其中22名研究人员需要细胞分选来支持他们的研究工作。这一联合奋进需要总共31个NIH补助金和至少6个额外的非NIH补助金（如VA Merit Review赠款）的资助。通过核心服务支持的研究涵盖了广泛的健康科学领域，包括传染病，神经生物学，血液学，心脏病学，肿瘤学，胃肠病学，内分泌学和遗传学。 总之，收购MoFlo XDP高速细胞分选机将使圣地亚哥CFAR / VMRF流式细胞术研究中心能够继续为当地学术研究界提供“最先进”的服务，以支持从基础生物学到转化医学研究再到药物开发的跨学科研究。 公共卫生关系：我们正在申请资金，以更换现有的旧的，主要的设备（细胞分选机）与一个新的更新模型，将位于一个中央核心实验室。该设备将为22名不同研究人员的研究工作提供支持，他们的工作对于开发传染病、癌症和影响血液、大脑、心脏、免疫、消化和内分泌系统的疾病的新疗法至关重要。