Flow Cytometry And Functional Immunology Research

流式细胞术和功能免疫学研究

• 批准号: 7278947
• 负责人: CELSA A SPINA
• 金额: $ 36.63万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2012-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7278947
• 关键词: Acquired Immunodeficiency Syndrome; Allergy and Immunology; Area; Award; Cell Separation; Cell physiology; Clinical Research; Communities; Consultations; Core Facility; Data Analyses; Environment; Equipment; Evaluation; Experimental Designs; Flow Cytometry; Foundations; Funding Agency; Goals; HIV; HIV Infections; Highly Active Antiretroviral Therapy; Immune; Immunology; Infection; Institutes; Medical Research; Medical center; Neurobiology; Opportunistic Infections; Pathogenesis; Pilot Projects; Principal Investigator; Purpose; Reagent; Regulation; Research; Research Infrastructure; Research Institute; Research Personnel; Research Project Grants; Research Support; Resources; Services; Technical Expertise; Technology; Time; Vaccines; Veterans; Viral; base; gene therapy clinical study; innovation; instrument; pre-clinical; response; vaccine development

The purpose of the Flow Cytometry and Functional Immunology Research Core is to provide a centralized resource of technical expertise and major equipment to support and enhance the experimental design and execution of basic and clinical research in HIV pathogenesis that require application of flow cytometric phenotypic analysis, cell sorting, and/or evaluation of specific immune cell functions. A prime goal is to provide the intellectual environment and the material resources to enable junior investigators to apply flow cytometry technology to their projects and to encourage established investigators to initiate innovative pilot studies. To achieve these objectives, expert consultation is provided through the Core Director, Associate Directors, and technical supervisor; major instruments are selected for complementary functions; equipment use is accessible through dedicated technician operators; services are provided on a recharge basis; generated data are analyzed, reduced, and diagramed; and fiscal support is given to pilot and exploratory research projects through the provision of research reagents and instrument time. CFAR resources have enabled the Core to support a wide array of HIV research encompassing basic projects on viral regulation and mechanisms of pathogenesis to preclinical development of vaccines and gene therapies, and clinical studies of primary infection, opportunistic infections, neurobiology, and immune and viral responses to vaccines and HAART. To maintain our flow cytometry facility to meet the needs of the San Diego academic research community, several funding sources have been integrated for cooperative support: the UCSD Center for AIDS Research (CFAR), the VA Research Center for AIDS and HIV Infection (RCAHI), and the Veterans Affairs Medical Research Foundation (VMRF). In addition to principal investigators based at UCSD and the VA Medical Center, investigators from The Scripps Research Institute, the Salk Institute, the La Jolla Institute of Allergy and Immunology, and the Burnham Institute also utilize the resources provided by our central facility. Local use of flow cytometric technology continues to grow and diversify, and the Core facility strives to keep up with the growing demand. The CFAR award remains an integral component in this endeavor. Application of flow cytometry to several areas of biomolecular research, in addition to expanded applications in clinical research, makes continued CFAR support crucial to providing the infrastructure to take advantage of new scientific opportunities in HIV research.

流式细胞术和功能免疫学研究核心的目的是提供一个集中的 技术专门知识和主要设备资源，以支持和加强实验设计， 开展需要应用流式细胞术的艾滋病毒发病机制的基础和临床研究 表型分析、细胞分选和/或特异性免疫细胞功能的评价。主要目标是 提供知识环境和物质资源，使初级调查人员能够应用流程 鼓励现有的研究人员启动创新的试点项目， 问题研究为实现这些目标，通过核心主任、协理主任和副主任提供专家咨询。 主任、技术主管;主要仪器选用功能互补; 设备使用可通过专门的技术人员操作员访问;服务通过充电提供 基础上;生成的数据进行分析，减少，和图解;并给予财政支持，试点和 通过提供研究试剂和仪器时间的探索性研究项目。CFAR 资源使核心基金能够支持广泛的艾滋病毒研究，包括以下基本项目： 病毒调控和致病机制对疫苗和基因临床前开发的影响 原发性感染、机会性感染、神经生物学、免疫和 对疫苗和HAART的病毒反应。 为了维护我们的流式细胞仪设施，以满足圣地亚哥学术研究界的需求， 几个资金来源已被整合为合作支持：加州大学圣地亚哥分校艾滋病中心 研究（CFAR），VA艾滋病和艾滋病毒感染研究中心（RCAHI）和退伍军人事务部 医学研究基金会（VMRF）。除了加州大学圣地亚哥分校和退伍军人事务部的主要研究人员外， 医学中心，斯克里普斯研究所，索尔克研究所，拉霍亚研究所的研究人员 过敏和免疫学，和伯纳姆研究所也利用我们的中央提供的资源， 设施。流式细胞技术的本地使用继续增长和多样化，核心设施努力 以满足日益增长的需求。CFAR奖仍然是这一奋进的一个组成部分。 流式细胞术在生物分子研究的几个领域中的应用， 在临床研究中的应用，使得持续的CFAR支持对于提供基础设施至关重要， 利用艾滋病毒研究中的新科学机会。